, Volume 18, Issue 3, pp 233–241 | Cite as

Metals content in placentas from moderate cigarette consumers: correlation with newborn birth weight

  • Ana Maria RoncoEmail author
  • Graciela Arguello
  • Luis Muñoz
  • Nuri Gras
  • Miguel Llanos


Cigarette consumption during pregnancy produces deleterious effects in both, mother and fetus, some of them due to the presence of toxic elements in cigarette smoke, such as cadmium. Placenta constitutes a dual-purpose specimen for evaluating the pollutant burden exerted on the mother as well as on the fetus. The main objective of this study was to establish a correlation between placental concentration and distribution of some metal elements and birth weight of neonates delivered by mothers, who were either moderate smokers or nonsmokers. Forty nonsmoking and moderate smoking pregnant women paired per age, parity, weight, height and body mass index were selected. Smoking was assessed by self-reported cigarette consumption during pregnancy and urine cotinine concentration before delivery. Placental metal concentrations were evaluated by atomic absorption spectrometry (copper and cadmium) and neutron activation analysis (zinc and iron). Newborns from smokers had lower birth weights compared to infants from nonsmokers. Birth weights were correlated with placental cadmium concentrations in both, smokers and nonsmokers. Placental zinc and cadmium of smokers were mainly located at the maternal side and their levels were higher than those found in nonsmoker’s placentas. In addition, all metal nutrient/pollutant ratios were decreased in the smoker group. In this first study performed in our region, we found that moderate smoking mothers deliver neonates with decreased birth weight and highly correlated to placental cadmium concentration. Decreased metal nutrient/pollutant ratios, a condition here found in smokers, may indicate a placental dysfunction, contributing to impair birth weight.


birth weight metals placenta smoking 


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  1. Allen, LH. 2000Anemia and iron deficiency: effects on pregnancy outcomeAm J Clin Nutr711280S1284SPubMedGoogle Scholar
  2. Allen, LH. 2001Biological mechanisms that might underlie iron’s effects on fetal growth and preterm birthJ Nutr131581S589SPubMedGoogle Scholar
  3. Aslam, N, McArdle, HJ. 1992Mechanism of zinc uptake by microvilli isolated from human term placentaJ Cell Physiol151533538CrossRefPubMedGoogle Scholar
  4. Atalah, E,  et al. 1997Propuesta de un nuevo estándar de evaluación nutricional de embarazadasRev Med Chile12514291436PubMedGoogle Scholar
  5. Bremner, I. 1991Nutritional and physiologic significance of metallothioneinMethods Enzymol2052535PubMedGoogle Scholar
  6. Bush, PG,  et al. 2000aA quantitative study on the effects of maternal smoking on placental morphology and cadmium concentrationPlacenta21247256CrossRefGoogle Scholar
  7. Bush, PG,  et al. 2000bMaternal cigarette smoking and oxygen diffusion across the placentaPlacenta21824833CrossRefGoogle Scholar
  8. Chaube, S,  et al. 1973Zinc and cadmium in normal human embryos and fetusesArch Environ Health26237240PubMedGoogle Scholar
  9. Chen, CY, Lin, TH. 1998Nickel toxicity to human term placenta: in vitro study on lipid peroxidationJ Toxicol Environ Health A83747Google Scholar
  10. Clausen, HV,  et al. 1999Stem villous arteries from the placentas of heavy smokers: functions and mechanical propertiesAm J Obstet Gynecol180476482PubMedGoogle Scholar
  11. Cliver, SP,  et al. 1995The effect of cigarette smoking on neonatal anthropometrics measurementsObstet Gynecol85625630CrossRefPubMedGoogle Scholar
  12. Demir, R,  et al. 1994Structural changes in placental barrier of smoking motherA quantitative and ultrastructural study. Pathol Res Prac190656667Google Scholar
  13. Dempsey, D,  et al. 2002Accelerated metabolism of nicotine and cotinine in pregnant smokersJ Pharmacol Exp Ther301594598CrossRefPubMedGoogle Scholar
  14. Gambling, L,  et al. 2003Iron and copper interactions in development and the effect on pregnancy outcomeJ Nutr1331554S1556SPubMedGoogle Scholar
  15. Garrick, MD,  et al. 2003DMT-1: A mammalian transporter for multiple metalsBiometals164154CrossRefPubMedGoogle Scholar
  16. Georgieff, MK,  et al. 2000Identification and localization of divalent metal transporter-1 (DMT-1) in term human placentaPlacenta21799804CrossRefPubMedGoogle Scholar
  17. Godfrey, KM,  et al. 1991The effect of maternal anaemia and iron deficiency on the ratio of fetal weight to placental weightBr J Obstet Gynaecol98886891PubMedGoogle Scholar
  18. Goyer, R, Cherian, MG. 1992Role of metallothionein in human placenta and rats exposed to cadmiumIARC Sci Publ118239247PubMedGoogle Scholar
  19. Haworth, JC,  et al. 1980Relation of maternal cigarette smoking, obesity and energy consumption to infant sizeAm J Obstet Gynecol13811851189PubMedGoogle Scholar
  20. Hindmarsh, PC,  et al. 2000Effect of early maternal iron stores on placental weight and structureLancet356719723CrossRefPubMedGoogle Scholar
  21. IJzerman, RG,  et al. 2002The association between birth weight and capillary recruitment is independent of blood pressure and insulin sensitivity: A study in prepubertal childrenJ Hypertens2019571963CrossRefPubMedGoogle Scholar
  22. Iyengar, GV,  et al. 2001aHuman placenta as a “dual” biomarker for monitoring fetal and maternal environment with special reference to potentially toxic elements. Part 1: Physiology, function and sampling of placenta for element characterization.Sci Total Environ280195206CrossRefGoogle Scholar
  23. Iyengar, G,  et al. 2001bHuman placenta as a dual biomarker for monitoring fetal and maternal environment with special reference to potentially toxic trace elements. Part 2: Essential minor; trace and other (non-essential) elements in human placenta.sSci Total Environ280207219CrossRefGoogle Scholar
  24. Iyengar, G,  et al. 2001cHuman placenta as a dual biomarker for monitoring fetal and maternal environment with special reference to potentially toxic trace elements. Part 3: Toxic trace elements in placenta and placenta as a biomarker for trace elements.Sci Total Environ280221238CrossRefGoogle Scholar
  25. Jones, JE, Jose, PA 2004Neonatal blood pressure regulationSemin Perinatol28141148CrossRefPubMedGoogle Scholar
  26. Kantola, M,  et al. 2000Accumulation of cadmium, zinc and copper in maternal blood and developmental placental tissue: differences between Finland, Estonia and StPetersburg. Environ Res835666Google Scholar
  27. Keen, CL,  et al. 1998Effect of copper deficiency on prenatal development and pregnancy outcomeAm J Clin Nutr671003S1011SPubMedGoogle Scholar
  28. Kendrick, JS,  et al. 1996Women and smokingAm J Obstet Gynecol175528535CrossRefPubMedGoogle Scholar
  29. Kuhnert, BR,  et al. 1987The relationship between cadmium, zinc, and birth weight in pregnant women who smokeAm J Obstet Gynecol15712471251PubMedGoogle Scholar
  30. Kuhnert, BR,  et al. 1988aAssociations between placental cadmium and zinc and age and parity in pregnant women who smokeObstet Gynecol716770Google Scholar
  31. Kuhnert, BR,  et al. 1988bThe effect of maternal smoking on the relationship between maternal and fetal Zn status and infant birth weightJ Am College Nutr7309316Google Scholar
  32. Lagerkvist, BJ,  et al. 1996Is placenta a good indicator of cadmium and lead exposure?Arch Environ Health51389394PubMedGoogle Scholar
  33. Lehman, LD, Poisner, AM. 1984Induction of metallothionein synthesis in cultured human trophoblasts by cadmium and ZnJ. Toxicol Environ Health14419432PubMedGoogle Scholar
  34. Lindley, AA,  et al. 2000Effect of continuing or stopping smoking during pregnancy on infant birth weight, crown–heel length, head circumference, ponderal index, and brain: body weight ratioAm J Epidemiol152219225CrossRefPubMedGoogle Scholar
  35. Manci, EA, Blackburn, WR. 1987Regional variations in the levels of zinc, iron, copper and calcium in the term human placentaPlacenta8497502PubMedGoogle Scholar
  36. Mamelle, N,  et al. 2001Definition of fetal growth restriction according to constitutional growth potentialBiol Neonate80277285CrossRefPubMedGoogle Scholar
  37. Miller, RK,  et al. 1988Biological monitoring of the human placentaClarkson, TWNordberg, GFSager, PR eds. Biological monitoring of toxic metalsPlenum PressNew York, NY567602Google Scholar
  38. Nasrat, H,  et al. 1992Midpregnancy plasma zinc in normal and growth retarded fetuses- a preliminary studyBr J Obstet Gynaecol99646650PubMedGoogle Scholar
  39. Piasek, M,  et al. 2001Placental cadmium and progesterone concentrations in cigarette smokersReprod Toxicol15673681CrossRefPubMedGoogle Scholar
  40. Roquer, J,  et al. 1995Influence on fetal growth of exposure to tobacco smoke during pregnancyActa Pediatr84118121Google Scholar
  41. Reichrtova, E,  et al. 1998aSites of lead and nickel accumulation in the placental tissueHum Exp Toxicol17176181CrossRefGoogle Scholar
  42. Reichrtova, E,  et al. 1998bContents and localization of heavy metals in human placentaFresenius J Anal Chem361362364CrossRefGoogle Scholar
  43. Ronco AM et al. 2004 Increased levels of metallothionein in placenta of smokers. Toxicology (in press).Google Scholar
  44. Salgueiro, MJ,  et al. 2002The role of zinc in the growth and development of childrenNutrition18510519CrossRefPubMedGoogle Scholar
  45. Schneider, BD, Leibold, EA. 2003Effects on iron regulatory protein regulation on iron homeostasis during hypoxiaBlood10234043411CrossRefPubMedGoogle Scholar
  46. Tallkvist, J,  et al. 2003Effect of iron treatment on nickel absorption and gene expression of the divalent metal transporter (DMT1) by human intestinal Caco-2 cellsPharmacol Toxicol92121124CrossRefPubMedGoogle Scholar
  47. Templeton, DM, Lui, Y. 2003Genetic regulation of cell function in response to iron overload or chelationBiochim Biophys Acta1619113124PubMedGoogle Scholar
  48. Torjussen, ,  et al. 2003Cigarette smoking and nickel exposureJ Environ Monit5198201CrossRefPubMedGoogle Scholar
  49. Vio, F, Salazar, G. 1997Tabaquismo pasivoRev Chil Pediatr68139140Google Scholar
  50. Wallace, JM,  et al. 2004Nutritionally-mediated placental growth restriction in the growing adolescent: Consequences for the fetusBiol Reprod7110551062CrossRefPubMedGoogle Scholar
  51. Wen, SW,  et al. 1990Intrauterine growth retardation and preterm delivery: Prenatal risk factors in an indigent populationAm J Obstet Gynecol162213218PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Ana Maria Ronco
    • 1
    Email author
  • Graciela Arguello
    • 1
  • Luis Muñoz
    • 2
  • Nuri Gras
    • 2
  • Miguel Llanos
    • 1
  1. 1.Laboratorio de Hormonas y Receptores, Instituto de Nutrición y Tecnología de los Alimentos, INTAUniversidad de ChileSantiagoChile
  2. 2.Comisión Chilena de Energía NuclearCCHEN, La ReinaSantiagoChile

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