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Evidence for phosphorus limitation in high-elevation unvegetated soils, Niwot Ridge, Colorado

  • Clifton P. Bueno de Mesquita
  • Laurel M. Brigham
  • Pacifica Sommers
  • Dorota L. Porazinska
  • Emily C. Farrer
  • John L. Darcy
  • Katharine N. Suding
  • Steven K. SchmidtEmail author
Article
  • 54 Downloads

Abstract

A key challenge to understanding the effects of climate change and nutrient deposition on ecosystem functioning is our lack of knowledge about nutrient limitations of heterotrophic and phototrophic microbial communities. This is especially true in high elevation ecosystems where it has been shown that earlier melt-out of snow beds and glacial retreat is allowing photosynthetic microbes and plants to move into previously unvegetated areas. We used landscape-level analyses of microbial enzyme stoichiometries combined with soil microcosm fertilization studies to determine which nutrients are limiting to microbes in plant-free or sparsely vegetated, snow bed areas of the Colorado Front Range. Both of these independent approaches indicated that the ultimate limiting nutrient in unvegetated and sparsely vegetated soils is phosphorus (P) for phototrophic microbes, with co-limitation by carbon (C) for the entire microbial community. In contrast, vegetated soils in the same watersheds showed more balanced nitrogen (N), P and C co-limitation similar to patterns seen in other plant-dominated ecosystems. In microcosm experiments, P additions resulted in increased growth rates and percent cover by phototrophs, whereas N additions decreased the relative abundances of phototrophs. Taken together, our findings indicate that the colonization of high elevation ecosystems being impacted by N deposition and climate warming will likely be constrained by P limitation of both heterotrophic and phototrophic microbes and by negative impacts of N on microbial phototrophs. These effects may in turn limit the ability of these fragile ecosystems to immobilize inputs of atmospheric N causing increased runoff of excess N to downstream ecosystems.

Keywords

Cyanobacteria Extreme environments Glacial retreat High-elevation soils Microbial community assembly Microbial succession Phosphorus limitation 

Notes

Acknowledgements

We thank A.J. King, J.G. Smith, S.A. Sartwell, J. Anderson Huxley, M.J. Spasojevic, C.T. White, A.F. Meyer, S.P. O’Neill, B. Todd, and M. Weintraub for field and laboratory assistance.

Funding

Funding was provided by NSF grants for studying microbial community assembly and plant migration to higher elevations (DEB-1258160, DEB-1457827 and DEB-1637686), and the Niwot Ridge LTER program (DEB-1027341). Logistical support was provided by the CU Mountain Research Station and Niwot Ridge LTER program.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Clifton P. Bueno de Mesquita
    • 1
    • 2
  • Laurel M. Brigham
    • 1
    • 2
  • Pacifica Sommers
    • 1
  • Dorota L. Porazinska
    • 3
  • Emily C. Farrer
    • 4
  • John L. Darcy
    • 5
  • Katharine N. Suding
    • 1
    • 2
  • Steven K. Schmidt
    • 1
    Email author
  1. 1.Department of Ecology and Evolutionary BiologyUniversity of ColoradoBoulderUSA
  2. 2.Institute of Arctic and Alpine ResearchUniversity of ColoradoBoulderUSA
  3. 3.Department of Entomology and NematologyUniversity of FloridaGainesvilleUSA
  4. 4.Department of Ecology and Evolutionary BiologyTulane UniversityNew OrleansUSA
  5. 5.Division of Biomedical Informatics and Personalized Medicine, School of MedicineUniversity of Colorado Denver Anschutz Medical CampusAuroraUSA

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