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Biogeochemistry

, Volume 140, Issue 1, pp 111–125 | Cite as

Abundance of lipids in differently sized aggregates depends on their chemical composition

  • Gerrit Angst
  • Klaas G. J. Nierop
  • Šárka Angst
  • Jan Frouz
Article

Abstract

Evidence for a vital role of soil mineral matrix interactions in lipid preservation is steadily increasing. However, it remains unclear whether solvent-extractable (‘free’) or hydrolyzable (‘bound’) lipids, including molecular proxies, e.g., for cutin and suberin, are similarly affected by different stabilization mechanisms in soil (i.e., aggregation or organo-mineral association). To provide insights into the effect of these stabilization mechanisms on lipid composition and preservation, we investigated free and bound lipids in particulate and mineral soil fractions, deriving from sand- and silt-/clay-sized aggregates from a forest subsoil. While free lipids accumulated in sand-sized aggregates, the more complex bound lipids accumulated in silt- and clay-sized aggregates, particularly in the respective mineral fractions < 6.3 µm (fine silt and clay). The presence of both, cutin and suberin markers indicated input of leaf- and root-derived organic matter to the subsoil. Yet, our cutin marker (9,10,ω-trihydroxyoctadecanoic acid) was not extracted from the mineral aggregate compartments < 6.3 µm, perhaps due to its chemical structure (i.e., cross-linking via several hydroxy groups, and thus higher ‘stability’, in macromolecular structures). Combined, these results suggest that the chemical composition of lipids (and likely also that of other soil organic matter compounds) governs interaction with their environment, such as accumulation in aggregates or association with mineral soil compartments, and thus indirectly influences their persistence in soil.

Keywords

Cutin Suberin Alnus glutinosa L. Organo-mineral association Particulate organic matter Subsoil 

Notes

Acknowledgements

This work was realized within the Program for Research and Mobility Support of Starting Researchers of the Czech Academy of Sciences (Grant Number MSM200961705) and with support of MEYS CZ Grant Numbers: LM2015075 and EF16_013/0001782 – SoWa Ecosystems Research, and the Czech Science Foundation (Grant 18-24138S). We would like to thank Petr Kotas for support in chromatogram evaluation, Tomáš Picek for help with sample preparation, Anita van Leeuwen-Tolboom for XRD measurements, and Katja Heister and Francien Peterse for facilitating the Geolab infrastructure in Utrecht.

Supplementary material

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Supplementary material 3 (TIFF 841 kb)

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© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Biology Centre of the Czech Academy of SciencesInstitute of Soil Biology & SoWa Research InfrastructureČeské BudějoviceCzech Republic
  2. 2.Geolab, Faculty of GeosciencesUtrecht UniversityUtrechtThe Netherlands
  3. 3.Faculty of Science, Institute for Environmental StudiesCharles UniversityPragueCzech Republic

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