, Volume 137, Issue 3, pp 307–320 | Cite as

Methane fluxes from tree stems and soils along a habitat gradient

  • Scott L. Pitz
  • J. Patrick Megonigal
  • Chih-Han Chang
  • Katalin Szlavecz


Forests are major sources of terrestrial CH4 and CO2 fluxes but not all surfaces within forests have been measured and accounted for. Stem respiration is a well-known source of CO2, but more recently tree stems have been shown to be sources of CH4 in wetlands and upland habitats. A study transect was established along a natural moisture gradient, with one end anchored in a forested wetland, the other in an upland forest and a transitional zone at the midpoint. Stem and soil fluxes of CH4 and CO2 were measured using static chambers during the 2013 and 2014 growing seasons, from May to October. Mean stem CH4 emissions were 68.8 ± 13.0 (mean ± standard error), 180.7 ± 55.2 and 567.9 ± 174.5 µg m−2 h−1 for the upland, transitional and wetland habitats, respectively. Mean soil methane fluxes in the upland, transitional and wetland were − 64.8 ± 6.2, 7.4 ± 25.0 and 190.0 ± 123.0 µg m−2 h−1, respectively. Measureable CH4 fluxes from tree stems were not always observed, but every individual tree in our experiment released measureable CH4 flux at some point during the study period. These results indicate that tree stems represent overlooked sources of CH4 in forested habitats and warrant investigation to further refine CH4 budgets and inventories.


Trees Methane Wetland forest Upland forest C cycle 



This study was supported by Grants from the Department of Energy (DE-SC0008165), the National Science Foundation (ACI-1244820, EAGER NEON EF-1550795, ERC-MIRTHE EEC-0540832), and the Department of Earth and Planetary Sciences Summer Field funds. We thank Jess Parker, Anand Gnanadesikan, Lisa Schile, and members of the GCREW Lab for their advice and useful suggestions throughout the study. We are thankful for all the help that Mike Bernard, Jacob Rode, Adam Dec, Andy Sample and Kyle King provided in the field. Anand Gnanadesikan and two anonymous reviewers provided helpful comment on earlier versions of the manuscript.

Supplementary material

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  1. Aydin M et al (2011) Recent decreases in fossil-fuel emissions of ethane and methane derived from firn air. Nature 476:198–201. CrossRefGoogle Scholar
  2. Baird AJ, Stamp I, Heppell CM, Green SM (2010) CH4 flux from peatlands: a new measurement method. Ecohydrology 3:360–367. CrossRefGoogle Scholar
  3. Brown MJ, Parker GG (1994) Canopy light transmittance in a chronosequence of mixed-species deciduous forests. Can J For Res 24:1694–1703. CrossRefGoogle Scholar
  4. Brush GS, Lenk C, Smith J (1980) The natural forests of Maryland - an explanation of the vegetation map of Maryland. Ecol Monogr 50:77. CrossRefGoogle Scholar
  5. Bushong FW (1907) Composition of gas from cottonwood trees. Trans Kans Acad Sci 21:53. CrossRefGoogle Scholar
  6. Conrad R (2007) Microbial ecology of methanogens and methanotrophs. Adv Agron 96:1–63. CrossRefGoogle Scholar
  7. Covey KR, Wood SA, Warren RJ, Lee X, Bradford MA (2012) Elevated methane concentrations in trees of an upland forest. Geophys Res Lett. Google Scholar
  8. Covey KR et al (2016) Greenhouse trace gases in deadwood. Biogeochemistry 130:215–226. CrossRefGoogle Scholar
  9. Dacey JWH, Klug MJ (1979) Methane efflux from lake-sediments through water lilies. Science 203:1253–1255. CrossRefGoogle Scholar
  10. Davidson EA, Belk E, Boone RD (1998) Soil water content and temperature as independent or confounded factors controlling soil respiration in a temperate mixed hardwood forest. Global Change Biol 4:217–227. CrossRefGoogle Scholar
  11. FAO (2016) State of the world’s forests 2016. FAO, RomeGoogle Scholar
  12. Fung I, Matthews E, Lerner J (1987) Atmospheric methane response to biogenic sources - results from a 3-D atmospheric tracer model. Abstr Pap Am Chem Soc 193:6-GeocGoogle Scholar
  13. Garnet KN, Megonigal JP, Litchfield C, Taylor GE (2005) Physiological control of leaf methane emission from wetland plants. Aquat Bot 81:141–155. CrossRefGoogle Scholar
  14. Gauci V, Gowing DJG, Hornibrook ERC, Davis JM, Dise NB (2010) Woody stem methane emission in mature wetland alder trees. Atmos Environ 44:2157–2160. CrossRefGoogle Scholar
  15. Hagedorn F, Bellamy P (2011) Hot spots and hot moments for greenhouse gas emissions from soils. Soil carbon in sensitive European ecosystems, vol 13. From science to land management. Wiley, hoboken, p 32. Google Scholar
  16. Higman D (1968) An ecologically annotated checklist of the vascular flora at the Chesapeake Bay Center for field biology, with keys. Smithsonian Institution, Washington DCGoogle Scholar
  17. Hothorn T, Bretz F, Westfall P (2008) Simultaneous inference in general parametric models. Biom J 50:346–363. CrossRefGoogle Scholar
  18. IPCC (2013) Climate change 2013: the physical science basis. Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change. Cambridge University Press, Cambridge and New York, NY.
  19. Keppler F, Hamilton JTG, Brass M, Rockmann T (2006) Methane emissions from terrestrial plants under aerobic conditions. Nature 439:187–191. CrossRefGoogle Scholar
  20. Keppler F, Hamilton JTG, McRoberts WC, Vigano I, Brass M, Rockmann T (2008) Methoxyl groups of plant pectin as a precursor of atmospheric methane: evidence from deuterium labelling studies. New Phytol 178:808–814. CrossRefGoogle Scholar
  21. Kirschke S et al (2013) Three decades of global methane sources and sinks. Nat Geosci 6:813–823. CrossRefGoogle Scholar
  22. Kuzyakov Y, Blagodatskaya E (2015) Microbial hotspots and hot moments in soil: concept & review. Soil Biol Biochem 83:184–199. CrossRefGoogle Scholar
  23. Machacova K et al (2016) Pinus sylvestris as a missing source of nitrous oxide and methane in boreal forest. Sci Rep 6:23410.
  24. Maier M, Machacova K, Lang F, Svobodova K, Urban O (2017) Combining soil and tree-stem flux measurements and soil gas profiles to understand CH4 pathways in Fagus sylvatica forests. J Plant Nutr Soil Sci. Google Scholar
  25. McClain ME et al (2003) Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems. Ecosystems 6:301–312. CrossRefGoogle Scholar
  26. Megonigal JP, Guenther AB (2008) Methane emissions from upland forest soils and vegetation. Tree Physiol 28:491–498CrossRefGoogle Scholar
  27. Megonigal JP, Conner WH, Kroeger S, Sharitz RR (1997) Aboveground production in Southeastern floodplain forests: a test of the subsidy-stress hypothesis. Ecology 78:370–384Google Scholar
  28. Megonigal JP, Hines ME, Visscher PT (2004) Anaerobic metabolism: linkages to trace gases and aerobic processes. In: Schlesinger WH (ed) Biogeochemistry, vol 8, 1st edn. Treatise on geochemistry. Elsevier, Oxford, pp 317–424Google Scholar
  29. Moyano FE, Manzoni S, Chenu C (2013) Responses of soil heterotrophic respiration to moisture availability: an exploration of processes and models. Soil Biol Biochem 59:72–85. CrossRefGoogle Scholar
  30. Natural-Resources-Conservation-Service (2016) Web soil survey. Accessed 15 Jan 2017
  31. Neubauer SC, Megonigal JP (2015) Moving beyond global warming potentials to quantify the climatic role of ecosystems. Ecosystems 18:1000–1013. CrossRefGoogle Scholar
  32. Nisbet EG et al (2016) Rising atmospheric methane: 2007–2014 growth and isotopic shift. Glob Biogeochem Cycles 30:1356–1370. CrossRefGoogle Scholar
  33. Pangala SR, Moore S, Hornibrook ERC, Gauci V (2013) Trees are major conduits for methane egress from tropical forested wetlands. New Phytol 197:524–531. CrossRefGoogle Scholar
  34. Pangala SR, Gowing DJ, Hornibrook ERC, Gauci V (2014) Controls on methane emissions from Alnus glutinosa saplings. New Phytol 201:887–896. CrossRefGoogle Scholar
  35. Pangala SR, Hornibrook ERC, Gowing DJ, Gauci V (2015) The contribution of trees to ecosystem methane emissions in a temperate forested wetland. Glob Change Biol. Google Scholar
  36. Parker GG, Tibbs DJ (2004) Structural phenology of the leaf community in the canopy of a Liriodendron tulipifera L. forest in Maryland, USA. For Sci 50:387–397Google Scholar
  37. Pitz S, Megonigal JP (2017) Temperate forest methane sink diminished by tree emissions. New Phytol. Google Scholar
  38. Pulliam WM (1992) Methane emissions from cypress knees in a southeastern floodplain swamp. Oecologia 91:126–128CrossRefGoogle Scholar
  39. R Core Team (2014) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, AustriaGoogle Scholar
  40. Rusch H, Rennenberg H (1998) Black alder (Alnus glutinosa (L.) Gaertn.) trees mediate methane and nitrous oxide emission from the soil to the atmosphere. Plant Soil 201:1–7. CrossRefGoogle Scholar
  41. Ryan MG (1990) Growth and maintenance respiration in stems of Pinus Contorta and Picea Engelmannii. Can J For Res 20:48–57. CrossRefGoogle Scholar
  42. Saunois M et al (2016) The global methane budget 2000–2012. Earth Syst Sci Data 8:697–751. CrossRefGoogle Scholar
  43. Schenk HJ, Jackson RB (2002) Rooting depths, lateral root spreads and below-ground/above-ground allometries of plants in water-limited ecosystems. J Ecol 90:480–494. CrossRefGoogle Scholar
  44. Siegenthaler A, Welch B, Pangala SR, Peacock M, Gauci V (2016) Technical note: semi-rigid chambers for methane gas flux measurements on tree stems. Biogeosciences 13:1197–1207. CrossRefGoogle Scholar
  45. Suseela V, Conant RT, Wallenstein MD, Dukes JS (2012) Effects of soil moisture on the temperature sensitivity of heterotrophic respiration vary seasonally in an old-field climate change experiment. Glob Change Biol 18:336–348. CrossRefGoogle Scholar
  46. Terazawa K, Ishizuka S, Sakatac T, Yamada K, Takahashi M (2007) Methane emissions from stems of Fraxinus mandshurica var. japonica trees in a floodplain forest. Soil Biol Biochem 39:2689–2692. CrossRefGoogle Scholar
  47. Terazawa K, Yamada K, Ohno Y, Sakata T, Ishizuka S (2015) Spatial and temporal variability in methane emissions from tree stems of Fraxinus mandshurica in a cool-temperate floodplain forest. Biogeochemistry 123:349–362. CrossRefGoogle Scholar
  48. Tiner RW, Burke DG (1995) Wetlands of maryland. US Fish and Wildlife Service, Ecological Services, Region 5, Hadley, and Maryland Department of Natural Resources, AnnapolisGoogle Scholar
  49. Ullah S, Moore TR (2011) Biogeochemical controls on methane, nitrous oxide, and carbon dioxide fluxes from deciduous forest soils in eastern Canada. J Geophys Res Biogeosci. Google Scholar
  50. Vann CD, Megonigal JP (2003) Elevated CO2 and water depth regulation of methane emissions: comparison of woody and non-woody wetland plant species. Biogeochemistry 63:117–134. CrossRefGoogle Scholar
  51. Vigano I, van Weelden H, Holzinger R, Keppler F, McLeod A, Rockmann T (2008) Effect of UV radiation and temperature on the emission of methane from plant biomass and structural components. Biogeosciences 5:937–947CrossRefGoogle Scholar
  52. Wang ZP et al (2016) Methane emissions from the trunks of living trees on upland soils. New Phytol 211:429–439. CrossRefGoogle Scholar
  53. Warner DL, Villarreal S, McWilliams K, Inamdar S, Vargas R (2017) Carbon dioxide and methane fluxes from tree stems, coarse woody debris, and soils in an upland temperate forest. Ecosystems. Google Scholar
  54. Yesilonis I, Szlavecz K, Pouyat R, Whigham D, Xia L (2016) Historical land use and stand age effects on forest soil properties in the Mid-Atlantic US. For Ecol Manag 370:83–92. CrossRefGoogle Scholar
  55. Yu KW, Faulkner SP, Baldwin MJ (2008) Effect of hydrological conditions on nitrous oxide, methane, and carbon dioxide dynamics in a bottomland hardwood forest and its implication for soil carbon sequestration. Glob Change Biol 14:798–812. CrossRefGoogle Scholar
  56. Zeikus JG, Ward JC (1974) Methane formation in living trees: a microbial origin. Science 184:1181–1183. CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Johns Hopkins UniversityBaltimoreUSA
  2. 2.Smithsonian Environmental Research CenterEdgewaterUSA

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