Biogeochemistry

, Volume 134, Issue 1–2, pp 77–94 | Cite as

Carbon quantity and quality drives variation in cave microbial communities and regulates Mn(II) oxidation

  • Mara L. C. Cloutier
  • Sarah K. Carmichael
  • Michael A. Carson
  • Michael D. Madritch
  • Suzanna L. Bräuer
Article

Abstract

Cave ecosystems are carbon limited and thus are particularly susceptible to anthropogenic pollution. Yet, how carbon quality and quantity that can modulate the pathways and amount of Mn cycling in caves remains largely unknown. To explore Mn cycling, baseline bacterial, archaeal, and fungal communities associated with Mn(III/IV) oxide deposits were assessed in both relatively ‘pristine’ and anthropogenically impacted caves in the Appalachian Mountains (USA). Cave sites were then amended with various carbon sources that are commonly associated with anthropogenic input to determine whether they stimulate biotic Mn(II) oxidation in situ. Results revealed patterns between sites that had long-term exogenous carbon loading compared to sites that were relatively ‘pristine,’ particularly among Bacteria and Archaea. Carbon treatments that stimulated Mn(II) oxidation at several sites resulted in significant changes to the microbial communities, indicating that anthropogenic input can not only enhance biotic Mn(II) oxidation, but also shape community structure and diversity. Additional carbon sources amended with copper were incubated at various cave sites to test the role that Cu(II) plays in in situ biotic Mn(II) oxidation. Media supplemented with 100 µM Cu(II) inhibited bacterial Mn(II) oxidation but stimulated fungal Mn(II) oxidation, implicating fungal use of multicopper oxidase (MCO) enzymes but bacterial use of superoxide to oxidize Mn(II). In sites with low C:N ratios, the activity of the Mn(II) oxidizing enzyme manganese peroxidase (MnP) appears to be limited (particularly by MnP-utilizing Basidiomycetes and/or Zygomycetes).

Keywords

Manganese oxidation Caves Exogenous carbon Fungal communities Bacterial communities Enzyme 

Abbreviations

LBB

Leucoberbelin blue

MnP

Manganese peroxidase

MCO

Multicopper oxidase

NMDS

Non-metric multidimensional scaling

PCA

Principal component analysis

SOM

Soil organic matter

TC

Total carbon

TN

Total nitrogen

TOC

Total organic carbon

Supplementary material

10533_2017_343_MOESM1_ESM.pdf (2.7 mb)
Supplementary material 1 (PDF 2728 kb)

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Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  1. 1.Department of BiologyAppalachian State UniversityBooneUSA
  2. 2.Department of Ecosystem Science and ManagementPennsylvania State UniversityUniversity ParkUSA
  3. 3.Department of GeologyAppalachian State UniversityBooneUSA
  4. 4.Department of BiologyLaurentian UniversitySudburyUSA
  5. 5.The Vale Living with Lakes CentreSudburyUSA

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