, Volume 129, Issue 3, pp 273–289 | Cite as

Quantitative assessment of photo- and bio-reactivity of chromophoric and fluorescent dissolved organic matter from biomass and soil leachates and from surface waters in a subtropical wetland

  • Meilian Chen
  • Rudolf Jaffé


Dissolved organic matter (DOM) reactivity plays a central role in ecosystem function and the global carbon cycle. In this study, a suite of biomass and soil leachates together with surface water samples from the Florida coastal Everglades (FCE) were investigated to quantitatively assess the photo- and bio-reactivity of DOM via dissolved organic carbon (DOC), ultraviolet–visible absorbance (UV–Vis) measurements, and excitation-emission matrix (EEM) fluorescence combined with parallel factor analysis (PARAFAC). The decomposition patterns observed were diverse, but dominated by first order decay for most of the samples studied. The reactivity rate constants obtained based on a first order multi-pool kinetic model, suggest that the DOM in the Everglades is largely refractory on time scales relevant to this ecosystem (2 months). The relative reactivity of different fluorescent DOM pools determined as labile, semi-labile and refractory were in the order of biomass leachate > soil leachate > surface water. Photo-reactivity was found to be a more important process in controlling the fate of FDOM in this system, while FDOM was mainly refractory to bio-degradation. Degradation patterns and rate constants for samples of different origin displayed vast differences for the same PARAFAC component, suggesting a complex and heterogeneous composition of fluorophores for each component, most likely the result of different structures, speciation and conformation, and molecular weight distribution. In addition, a terrestrial humic-like and two protein-like components were found to have the potential to serve as indicators of photo-degradable and bioavailable DOM.


DOM Photodegradation Biodegradation Reactivity Kinetics Wetland 



This study was funded by NSF through the FCE-LTER program (DEB-1237517). The authors thank Wetland Ecosystem Laboratory at SERC for logistic support and the SERC Water Quality Laboratory for DOC analyses. MC thanks NSF for a Research Assistantship and the Department of Chemistry and Biochemistry for a Teaching Assistantship. Special thanks go to Drs. Y. Yamashita and N. Maie for their technical advice and helpful discussions during the performance of this work. Additional support for this study was provided through the George Barley Endowment. This is SERC contribution #807.

Supplementary material

10533_2016_231_MOESM1_ESM.docx (73 kb)
Supplementary material 1 (DOCX 72 kb)


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© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Department of Chemistry and Biochemistry, Southeast Environmental Research CenterFlorida International UniversityMiamiUSA

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