Using 2D NMR spectroscopy to assess effects of UV radiation on cell wall chemistry during litter decomposition
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Litter chemistry is one of the most studied controls on decomposition in terrestrial ecosystems. Solar radiation has been shown to increase litter decomposition rates in arid ecosystems through the process of photodegradation. However, it remains unclear how photodegradation affects litter chemistry, especially the abundance and composition of lignin, which is thought to play a key role in photodegradation. Using two-dimensional nuclear magnetic resonance (2D NMR) spectroscopic methods, we quantified the molecular-level changes in litter chemistry associated with photodegradation. Litter of Bromus diandrus was exposed in the field to two levels of radiation [with and without ultraviolet (UV) wavelengths] and two durations of exposure (2.5 months during summer, and 1 year). Through fiber analysis by sequential digestion, we found that the litter hemicellulose fraction decreased significantly from 31.6 to 24.9 % after 1 year of decomposition. In litter exposed for 1 year, the hemicellulose fraction was significantly lower in litter with UV exposure compared to litter without UV exposure (23.8 vs. 25.9 %). These results indicate that UV photodegradation has a small but significant effect on litter chemistry compared to other decomposition processes. Even though fiber analysis showed no loss of total lignin, 2D NMR analysis demonstrated that UV exposure reduced the major lignin structural units containing β-aryl ether inter-unit linkages by 9 % and decreased the relative abundance of lignin p-hydroxyphenyl units by 20 %. The 2D NMR analysis also revealed that lignin guaiacyl units were preferentially lost after 1 year of decomposition relative to the reference material, but no effects of UV exposure on guaiacyl units were observed. These results suggest that photodegradation causes partial degradation, not necessarily complete breakdown, of lignin structures. Our data also demonstrate that applications of 2D NMR methods are valuable for acquiring detailed information on lignin and polysaccharide chemistry during both biotic and abiotic decomposition processes.
KeywordsPhoto-oxidation Photo-mineralization Photo-priming Cellulose Dryland HSQC (heteronuclear single-quantum coherence)
We thank Dad Roux-Michollet, Keri Opalk, Sarah Liu, and Jerry Hu for their assistance in the field and laboratory. We thank Oliver Chadwick and Carla D’Antonio for their valuable comments on the experimental design and on this manuscript. We thank Kate McCurdy, Eric Massey, and the University of California’s Sedgwick Reserve for providing the study site. Comments from Associate Editor Marc Kramer and four anonymous reviewers greatly improved this manuscript. YL and JK were supported by the National Science Foundation under DEB-0935984 and DEB-1406501. JR and SK were supported by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494).
- Adair EC, Parton WJ, Del Grosso SJ, Silver WL, Harmon ME, Hall SA, Burke IC, Hart SC (2008) Simple three-pool model accurately describes patterns of long-term litter decomposition in diverse climates. Global Change Biol 14:2636–2660Google Scholar
- Brandt LA, Bohnet C, King JY (2009) Photochemically induced carbon dioxide production as a mechanism for carbon loss from plant litter in arid ecosystems. J Geophys Res 114:G02004Google Scholar
- Christman RF, Oglesby RT (1971) Microbiological degradation and the formation of humus. In: Sarkanen KV, Ludwig CH (eds) Lignins: Occurrence, Formation, Structure and Reactions. Wiley, New York, pp 769–793Google Scholar
- Petrik DL, Karlen SD, Cass CL, Padmakshan D, Lu F, Liu S, Le Bris P, Antelme S, Santoro N, Wilkerson CG, Sibout R, Lapierre C, Ralph J, Sedbrook JC (2014) p-Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon. Plant J 77:713–726CrossRefGoogle Scholar
- Ralph SA, Ralph J, Landucci LL (2004) NMR database of lignin and cell wall model compounds. November 2004. https://www.glbrc.org/databases_and_software/nmrdatabase/
- Van Soest PJ (1963) Use of detergents in analysis of fibrous feeds: a rapid method for the determination of fiber and lignin. J Ass Offic Agr Chem 46:829–835Google Scholar
- Yamagishi T, Yoshimoto T, Minami K (1970) Photodegradation of wood. VII. Isolation and photodegradation of O-acetylated xylan (hemicellulose) from Linden wood. J Jpn Wood Res Soc 16:87–91Google Scholar