Biogeochemistry

, Volume 113, Issue 1–3, pp 563–572 | Cite as

The kinetics of denitrification in permeable sediments

  • Victor Evrard
  • Ronnie N. Glud
  • Perran L. M. Cook
Article

Abstract

Permeable sediments comprise the majority of shelf sediments, yet the rates of denitrification remain highly uncertain in these environments. Computational models are increasingly being used to understand the dynamics of denitrification in permeable sediments, which are complex environments to study experimentally. The realistic implementation of such models requires reliable experimentally derived data on the kinetics of denitrification. Here we undertook measurements of denitrification kinetics as a function of nitrate concentration in carefully controlled flow through reactor experiments on sediments taken from six shallow coastal sites in Port Phillip Bay, Victoria, Australia. The results showed that denitrification commenced rapidly (within 30 min) after the onset of anoxia and the kinetics could be well described by Michaelis–Menten kinetics with half saturation constants (apparent Km) ranging between 1.5 and 19.8 μM, and maximum denitrification rate (Vmax) were in the range of 0.9–7.5 nmol mL−1 h−1. The production of N2 through anaerobic ammonium oxidation (anammox) was generally found to be less than 10 % of denitrification. Vmax were in the same range as previously reported in cohesive sediments despite organic carbon contents one order of magnitude lower for the sediments studied here. The ratio of sediment O2 consumption to Vmax was in the range of 0.02–0.09, and was on average much lower than the theoretical ratio of 0.8. As a consequence, models implemented with the theoretical ratio of 0.8 are likely to overestimate denitrification by a factor of ~3. The most likely explanation for this is that the microbial community is not able to instantaneously shift or optimally use a particular electron acceptor in the highly dynamic redox environment experienced in permeable sediments. In contrast to previous studies, we did not observe any significant rates of oxic denitrification.

Keywords

Permeable sediment Denitrification Anammox Michaelis–Menten Kinetics Oxic denitrification 

References

  1. Boudreau BP (1997) Diagenetic models and their implementation. Springer-Verlag, BerlinCrossRefGoogle Scholar
  2. Boudreau BP, Huettel M, Forster S, Jahnke RA, McLachlan A, Middelburg JJ, Nielsen P, Sansone F, Taghon GL, van Raaphorst W, Webster IT, Weslawski JM, Wiberg P, Sundby B (2001) Permeable marine sediments: overturning an old paradigm. EOS Trans Am Geophys Union 82:133–136Google Scholar
  3. Burdige DJ (2006) Geochemistry of marine sediments. Princeton University Press, PrincetonGoogle Scholar
  4. Burgin AJ, Hamilton SK (2007) Have we overemphasized the role of denitrification in aquatic ecosystems? A review of nitrate removal pathways. Front Ecol Environ 5(2):89–96CrossRefGoogle Scholar
  5. Canfield DE, Thamdrup B, Kristensen E (2005) Aquatic geomicrobiology. Elsevier, AmsterdamGoogle Scholar
  6. Cardenas MB, Cook PLM, Jiang HS, Traykovski P (2008) Constraining denitrification in permeable wave-influenced marine sediment using linked hydrodynamic and biogeochemical modeling. Earth Planet Sci Lett 275(1–2):127–137CrossRefGoogle Scholar
  7. Cook PLM, Wenzhöfer F, Rysgaard S, Galaktionov OS, Meysman FJR, Eyre BD, Cornwell JC, Huettel M, Glud RN (2006) Quantification of denitrification in permeable sediments: insights from a two dimensional simulation analysis and experimental data. Limnol Oceanogr Methods 4:294–307CrossRefGoogle Scholar
  8. Cook PLM, Wenzhöfer F, Glud RN, Janssen F, Huettel M (2007) Benthic solute exchange and carbon mineralisation in two subtidal sandy sediments: effect of flow. Limnol Oceanogr 52:1943–1963CrossRefGoogle Scholar
  9. Emery KO (1968) Relict sediments on continental shelves of the world. Am Assoc Pet Geol Bull 52:445–464Google Scholar
  10. Gao H, Schreiber F, Collins G, Jensen MM, Kostka JE, Lavik G, de Beer D, Zhou HY, Kuypers MMM (2010) Aerobic denitrification in permeable Wadden Sea sediments. ISME J 4(3):417–426CrossRefGoogle Scholar
  11. Gihring TM, Canion A, Riggs A, Huettel M, Kostka JE (2010) Denitrification in shallow, sublittoral Gulf of Mexico permeable sediments. Limnol Oceanogr 55(1):43–54CrossRefGoogle Scholar
  12. Gruber N, Galloway JN (2008) An Earth-system perspective of the global nitrogen cycle. Nature 451(7176):293–296CrossRefGoogle Scholar
  13. Howarth RW, Marino R (2006) Nitrogen as the limiting nutrient for eutrophication in coastal marine ecosystems: evolving views over three decades. Limnol Oceanogr 51(1):364–376CrossRefGoogle Scholar
  14. Huettel M, Webster IT (2001) Porewater flow in permeable sediments. In: Boudreau BP, Jørgensen BB (eds) The benthic boundary layer. Oxford University Press, Oxford, pp 144–179Google Scholar
  15. Joye SB, Smith SV, Hollibaugh JT, Paerl HW (1996) Estimating denitrification rates in estuarine sediments—a comparison of stoichiometric and acetylene based methods. Biogeochemistry 3:197–215CrossRefGoogle Scholar
  16. Kessler AJ, Glud RN, Cardenas MB, Larsen M, Bourke MF, Cook PLM (2012) Quantifying denitrification in rippled permeable sands through combined flume experiments and modeling. Limnol Oceanogr 57(4):1217–1232CrossRefGoogle Scholar
  17. Meysman FJR, Galaktionov OS, Cook PLM, Janssen F, Huettel M, Middelburg JJ (2007) Quantifying biologically and physically induced flow and tracer dynamics in permeable sediments. Biogeosciences 4:627–646CrossRefGoogle Scholar
  18. Middelburg JJ, Levin LA (2009) Coastal hypoxia and sediment biogeochemistry. Biogeosciences 6(7):1273–1293CrossRefGoogle Scholar
  19. Middelburg JJ, Soetaert K, Herman PMJ, Heip CHR (1996) Denitrification in marine sediments: a model study. Global Biogeochem Cycles 10(4):661–673CrossRefGoogle Scholar
  20. Middelburg JJ, Duarte CM, Gattuso JP (2005) Respiration in coastal benthic communities. In: del Giorgio PA, Williams PJ (eds) Respiration in aquatic ecosystems. Oxford University Press, Oxford, pp 206–224CrossRefGoogle Scholar
  21. Nielsen LP (1992) Denitrification in sediment determined from nitrogen isotope pairing. FEMS Microbiol Ecol 86:357–362CrossRefGoogle Scholar
  22. Precht E, Huettel M (2004) Rapid wave driven porewater exchange in a permeable coastal sediment. J Sea Res 51:93–107CrossRefGoogle Scholar
  23. Precht E, Franke U, Polerecky L, Huettel M (2004) Oxygen dynamics in permeable sediments with wave-driven porewater exchange. Limnol Oceanogr 49:693–705CrossRefGoogle Scholar
  24. Rao AMF, McCarthy MJ, Gardner WS, Jahnke RA (2007) Respiration and denitrification in permeable continental shelf deposits on the South Atlantic Bight: rates of carbon and nitrogen cycling from sediment column experiments. Cont Shelf Res 27(13):1801–1819CrossRefGoogle Scholar
  25. Reimers CE, Stecher HA, Taghon GL, Fuller CM, Huettel M, Rusch A, Ryckelynck N, Wild C (2004) In situ measurements of advective solute transport in permeable shelf sands. Cont Shelf Res 24(2):183–201CrossRefGoogle Scholar
  26. Reynolds WD (2008) Saturated hydraulic properties: laboratory methods. In: Carter MR, Gregorich EG (eds) Soil sampling and method of analysis. CRC Press, Boca Raton, pp 1013–1024Google Scholar
  27. Risgaard Petersen N, Nielsen LP, Rysgaard S, Dalsgaard T, Meyer RL (2003) Application of the isotope pairing technique in sediments where anammox and denitrification coexist. Limnol Oceanogr Methods 1:63–73CrossRefGoogle Scholar
  28. Roychoudhury AN, Viollier E, Van Cappellen P (1998) A plug flow-through reactor for studying biogeochemical reactions in undisturbed aquatic sediments. Appl Geochem 13(2):269–280CrossRefGoogle Scholar
  29. Soetaert K, Herman PMJ, Middelburg JJ (1996) A model of early diagenetic processes from the shelf to abyssal depths. Geochim Cosmochim Acta 60(6):1019–1040CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Victor Evrard
    • 1
  • Ronnie N. Glud
    • 2
    • 3
    • 4
  • Perran L. M. Cook
    • 1
  1. 1.Water Studies Centre, School of ChemistryMonash UniversityClaytonAustralia
  2. 2.Institute of Biology and Nordic Center for Earth Evolution (NordCEE)University of Southern DenmarkOdense MDenmark
  3. 3.Scottish Marine InstituteScottish Association for Marine ScienceObanScotland
  4. 4.Greenland Climate Research Centre (CO Greenland Institute of National Resources)NuukGreenland

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