Biogeochemistry

, Volume 112, Issue 1–3, pp 373–388 | Cite as

Calcium isotope fractionation in alpine plants

  • R. S. Hindshaw
  • B. C. Reynolds
  • J. G. Wiederhold
  • M. Kiczka
  • R. Kretzschmar
  • B. Bourdon
Article

Abstract

In order to develop Ca isotopes as a tracer for biogeochemical Ca cycling in terrestrial environments and for Ca utilisation in plants, stable calcium isotope ratios were measured in various species of alpine plants, including woody species, grasses and herbs. Analysis of plant parts (root, stem, leaf and flower samples) provided information on Ca isotope fractionation within plants and seasonal sampling of leaves revealed temporal variation in leaf Ca isotopic composition. There was significant Ca isotope fractionation between soil and root tissue \(\Updelta^{44/42}\hbox{Ca}_{\rm root-soil} \approx -0.40\,\permille\) in all investigated species, whereas Ca isotope fractionation between roots and leaves was species dependent. Samples of leaf tissue collected throughout the growing season also highlighted species differences: Ca isotope ratios increased with leaf age in woody species but remained constant in herbs and grasses. The Ca isotope fractionation between roots and soils can be explained by a preferential binding of light Ca isotopes to root adsorption sites. The observed differences in whole plant Ca isotopic compositions both within and between species may be attributed to several potential factors including root cation exchange capacity, the presence of a woody stem, the presence of Ca oxalate, and the levels of mycorrhizal infection. Thus, the impact of plants on the Ca biogeochemical cycle in soils, and ultimately the Ca isotope signature of the weathering flux from terrestrial environments, will depend on the species present and the stage of vegetation succession.

Keywords

Calcium Stable isotope fractionation Glacier forefield Alpine plants 

Notes

Acknowledgments

The authors would like to thank Gregory de Souza for insightful discussions regarding the stable isotope fractionation of Sr and Ca by plants, Monika Welc for measuring the mycorrhizal infection of root samples and providing the ‘chronosequence’ and mycorrhizal sporocap samples and Hans Göransson for his help in understanding plant biology and in choosing suitable plant species to study. We thank associate editor Steven Perakis and two reviewers for their constructive reviews of this manuscript. In particular we are indebted to Thomas Bullen for his very detailed and thought-provoking reviews which considerably improved this manuscript. This work was associated with the BigLink project of the Competence Center Environment and Sustainability of the ETH Domain (CCES) and was funded by ETH Research Grant No. 04/06-3.

References

  1. Bailey SW, Hornbeck JW, Driscoll CT, Gaudette HE (1996) Calcium inputs and transport in a base-poor forest ecosystem as interpreted by Sr isotopes. Water Resour Res 32:707–719CrossRefGoogle Scholar
  2. Balogh-Brunstad Z, Keller CK, Bormann BT, O’Brien R, Wang D, Hawley G (2008) Chemical weathering and chemical denudation dynamics through ecosystem development and disturbance. Glob Biogeochem Cycles 22:GB1007CrossRefGoogle Scholar
  3. Bernasconi SM, Bauder A, Bourdon B, Brunner I, Bünemann E, Christl I, Derungs N, Edwards P, Farinotti D, Frey B, Frossard E, Furrer G, Gierga M, Göransson H, Gülland K, Hagedorn F, Hajdas I, Hindshaw R, Ivy-Ochs S, Jansa J, Jonas T, Kiczka M, Kretzschmar R, Lemarchand E, Luster J, Magnusson J, Mitchell E, Olde Venterink H, Plötze M, Reynolds B, Smittenberg RH, Stähli M, Tamburini F, Tipper E, Wacker L, Welc M, Wiederhold JG, Zeyer J, Zimmermann S, Zumsteg A (2011) Chemical and biological gradients along the Damma Glacier soil chronosequence, Switzerland. Vadose Zone J 10:867–883CrossRefGoogle Scholar
  4. Bigeleisen J (1965) Chemistry of isotopes. Science 147:463–471CrossRefGoogle Scholar
  5. Blum JD, Dasch AA, Hamburg SP, Yanai RD, Arthur MA (2008) Use of foliar Ca/Sr discrimination and 87Sr/86Sr ratios to determine soil Ca sources to sugar maple foliage in a northern hardwood forest. Biogeochemistry 87:287–296CrossRefGoogle Scholar
  6. Bolou-Bi EB, Poszwa A, Leyval C, Vigier N (2010) Experimental determination of magnesium isotope fractionation during higher plant growth. Geochim Cosmochim Acta 74:2523–2537CrossRefGoogle Scholar
  7. Bormann BT, Wang D, Bormann FH, Benoit G, April R, Snyder MC (1998) Rapid, plant-induced weathering in an aggrading experimental ecosystem. Biogeochemistry 43:129–155CrossRefGoogle Scholar
  8. Bullen TD, Bailey SW (2005) Identifying calcium sources at an acid deposition-impacted spruce forest: a strontium isotope, alkaline earth element multi-tracer approach. Biogeochemistry 74:63–99CrossRefGoogle Scholar
  9. Capo RC, Stewart BW, Chadwick OA (1998) Strontium isotopes as tracers of ecosystem processes: theory and methods. Geoderma 82:197–225CrossRefGoogle Scholar
  10. Carugo O, Djinović K, Rizzi M (1993) Comparison of the co-ordinative behaviour of calcium(II) and magnesium(II) from crystallographic data. J Chem Soc Dalton Trans 14:2127–2135CrossRefGoogle Scholar
  11. Cenki-Tok B, Chabaux F, Lemarchand D, Schmitt AD, Pierret MC, Viville D, Stille P (2009) The impact of water–rock interaction and vegetation on calcium isotope fractionation in soil- and stream waters of a small, forested catchment (the Strengbach case). Geochim Cosmochim Acta 73:2215–2228CrossRefGoogle Scholar
  12. Clarkson DT (1984) Calcium transport between tissues and its distribution in the plant. Plant Cell Environ 7:449–456CrossRefGoogle Scholar
  13. Cobert F, Schmitt AD, Bourgeade P, Labolle F, Badot PM, Chabaux F, Stille P (2011) Experimental identification of Ca isotopic fractionations in higher plants. Geochim Cosmochim Acta 75:5467–5482CrossRefGoogle Scholar
  14. Dasch AA, Blum JD, Eagar C, Fahey TJ, Driscoll CT, Siccama TG (2006) The relative uptake of Ca and Sr into tree foliage using a whole-watershed calcium addition. Biogeochemistry 80:21–41CrossRefGoogle Scholar
  15. de Souza GF, Reynolds BC, Kiczka M, Bourdon B (2010) Evidence for mass-dependent isotopic fractionation of strontium in a glaciated granitic watershed. Geochim Cosmochim Acta 74:2596–2614CrossRefGoogle Scholar
  16. Doaigey AR (1991) Occurence, type, and location of calcium oxalate crystals in leaves and stems of 16 species of poisonous plants. Am J Bot 78:1608–1616CrossRefGoogle Scholar
  17. Drever JI (1994) The effect of land plants on weathering rates of silicate minerals. Geochim Cosmochim Acta 58:2325–2332CrossRefGoogle Scholar
  18. Drew MC, Biddulph O (1971) Effect of metabolic inhibitors and temperature on uptake and translocation of 45Ca and 42K by intact bean plants. Plant Physiol 48:426–432CrossRefGoogle Scholar
  19. Drouet T, Herbauts J (2008) Evaluation of the mobility and discrimination of Ca, Sr and Ba in forest ecosystems: consequence on the use of alkaline-earth element ratios as tracers of Ca. Plant Soil 302:105–124CrossRefGoogle Scholar
  20. Einspahr H, Bugg CE (1981) The geometry of calcium–carboxylate interactions in crystalline complexes. Acta Crystallogr B 37:1044–1052CrossRefGoogle Scholar
  21. Epstein E, Leggett JE (1954) The absorption of alkaline earth cations by barley roots: kinetics and mechanism. Am J Bot 41:785–791CrossRefGoogle Scholar
  22. Evans DE, Briars SA, Williams LE (1991) Active calcium transport by plant cell membranes. J Exp Bot 42:285–303CrossRefGoogle Scholar
  23. Ferguson IB, Bollard EG (1976) The movement of calcium in woody stems. Ann Bot 40:1057–1065Google Scholar
  24. Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84:489–500CrossRefGoogle Scholar
  25. Gorham E, Vitousek PM, Reiners WA (1979) The regulation of chemical budgets over the course of terrestrial ecosystem succession. Annu Rev Ecol Syst 10:53–84CrossRefGoogle Scholar
  26. Guha MM, Mitchell RL (1966) The trace and major element composition of the leaves of some deciduous trees II. Seasonal changes. Plant Soil 24:90–112CrossRefGoogle Scholar
  27. Haynes RJ (1980) Ion exchange properties of roots and ionic interactions within the root apoplasm: their role in ion accumulation by plants. Bot Rev 46:75–99CrossRefGoogle Scholar
  28. Hindshaw RS, Reynolds BC, Wiederhold JG, Kretzschmar R, Bourdon B (2011) Calcium isotopes in a proglacial weathering environment: Damma glacier, Switzerland. Geochim Cosmochim Acta 75:106–118CrossRefGoogle Scholar
  29. Holmden C, Bélanger N (2010) Ca isotope cycling in a forested ecosystem. Geochim Cosmochim Acta 74:995–1015CrossRefGoogle Scholar
  30. Hose E, Clarkson DT, Steudle E, Schreiber L, Hartung W (2001) The exodermis: a variable apoplastic barrier. J Exp Bot 52:2245–2264CrossRefGoogle Scholar
  31. Jalilehvand F, Spångberg D, Lindqvist-Reis P, Hermansson K, Persson I, Sandström M (2001) Hydration of the calcium ion. An EXAFS, large-angle X-ray scattering, and molecular dynamics simulation study. J Am Chem Soc 123:431–441CrossRefGoogle Scholar
  32. Johnson DW (1992) Base cation distribution and cycling. In: Johnson DW, Lindberg SE (eds) Atmospheric deposition and forest nutrient cycling, Springer, New York, pp 275–332CrossRefGoogle Scholar
  33. Karley AJ, White PJ (2009) Moving cationic minerals to edible tissues: potassium, magnesium, calcium. Curr Opin Plant Biol 12:291–298CrossRefGoogle Scholar
  34. Kaufman Katz A, Glusker JP, Beebe SA, Bock CW (1996) Calcium ion coordination: a comparison with that of beryllium, magnesium, and zinc. J Am Chem Soc 118:5752–5763CrossRefGoogle Scholar
  35. Kiczka M, Wiederhold JG, Kraemer SM, Bourdon B, Kretzschmar R (2010) Iron isotope fractionation during Fe uptake and translocation in alpine plants. Environ Sci Technol 44:6144–6150CrossRefGoogle Scholar
  36. Landolt E, Urbanska KM (2003) Our alpine flora. SAC Verlag, ChurGoogle Scholar
  37. Likens GE, Bormann FH, Johnson NM, Fisher DW, Pierce RS (1970) Effects of forest cutting and herbicide treatment on nutrient budgets in the Hubbard Brook watershed-ecosystem. Ecol Monogr 40:23–47CrossRefGoogle Scholar
  38. Likens GE, Driscoll CT, Buso DC, Siccama TG, Johnson CE, Lovett GM, Fahey TJ, Reiners WA, Ryan DF, Martin CW, Bailey SW (1998) The biogeochemistry of calcium at Hubbard Brook. Biogeochemistry 41:89–173CrossRefGoogle Scholar
  39. Marschner H (1995) Mineral nutrition of higher plants. Academic Press, LondonGoogle Scholar
  40. McLaughlin SB, Wimmer R (1999) Calcium physiology and terrestrial ecosystem processes. New Phytol 142:373–417CrossRefGoogle Scholar
  41. Page BD, Bullen TD, Mitchell MJ (2008) Influences of calcium availability and tree species on Ca isotope fractionation in soil and vegetation. Biogeochemistry 88:1–13CrossRefGoogle Scholar
  42. Pavlov M, Siegbahn PEM, Sandström M (1998) Hydration of beryllium, magnesium, calcium, and zinc ions using density functional theory. J Phys Chem A 102:219–228CrossRefGoogle Scholar
  43. Pett-Ridge JC, Derry LA, Barrows JK (2009) Ca/Sr and 87Sr/86Sr ratios as tracers of Ca and Sr cycling in the Rio Icacos watershed, Luquillo Mountains, Puerto Rico. Chem Geol 267:32–45CrossRefGoogle Scholar
  44. Poszwa A, Dambrine E, Pollier B, Atteia O (2000) A comparison between Ca and Sr cycling in forest ecosystems. Plant Soil 225:299–310CrossRefGoogle Scholar
  45. Rana BS, Singh SP, Singh RP (1989) Biomass and net primary productivity in cental Himalayan forests along an altitudinal gradient. For Ecol Manag 27:199–218CrossRefGoogle Scholar
  46. Russell WA, Papanastassiou DA (1978) Calcium isotope fractionation in ion-exchange chromatography. Anal Chem 50:1151–1154CrossRefGoogle Scholar
  47. Schmitt AD, Chabaux F, Stille P (2003) The calcium riverine and hydrothermal isotopic fluxes and the oceanic calcium mass balance. Earth Planet Sci Lett 6731:1–16Google Scholar
  48. Taylor AB, Velbel MA (1991) Geochemical mass balances and weathering rates in forested watersheds of the southern Blue Ridge II. Effects of botanical uptake terms. Geoderma 51:29–50Google Scholar
  49. Tipper ET, Galy A, Bickle MJ (2006) Riverine evidence for a fractionated reservoir of Ca and Mg on the continents: implications for the oceanic Ca cycle. Earth Planet Sci Lett 247:267–279CrossRefGoogle Scholar
  50. Tipper ET, Galy A, Bickle MJ (2008) Calcium and magnesium isotope systematics in rivers draining the Himalaya–Tibetan-Plateau region: lithological or fractionation control. Geochim Cosmochim Acta 72:1057–1075CrossRefGoogle Scholar
  51. Vande Geijn SC, Petit CM (1979) Transport of divalent cations: Cation exchange capacity of intact xylem vessels. Plant Physiol 64:954–958CrossRefGoogle Scholar
  52. Viers J, Oliva P, Nonell A, Gélabert A, Sonke JE, Freydier R, Gainville R, Dupré B (2007) Evidence of Zn isotopic fractionation in a soil–plant system of a pristine tropical watershed (Nsimi, Cameroon). Chem Geol 239:124–137CrossRefGoogle Scholar
  53. White PJ, Broadley MR (2003) Calcium in plants. Ann Bot 92:487–511CrossRefGoogle Scholar
  54. Wiegand BA, Chadwick OA, Vitousek PM, Wooden JL (2005) Ca cycling and isotopic fluxes in forested ecosystems in Hawaii. Geophys Res Lett 32:L11404Google Scholar
  55. Wong A, Howes AP, Dupree R, Smith ME (2006) Natural abundance 43Ca NMR study of calcium-containing organic solids: a model study for Ca-binding biomaterials. Chem Phys Lett 427:201–205CrossRefGoogle Scholar
  56. WRB (2006) World Reference Base for Soil Resources 2006—a framework for international classification, correlation and communication. 103, Food and Agriculture Organization of the United Nations, Rome, ItalyGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • R. S. Hindshaw
    • 1
    • 2
  • B. C. Reynolds
    • 1
  • J. G. Wiederhold
    • 1
    • 2
  • M. Kiczka
    • 1
    • 2
  • R. Kretzschmar
    • 2
  • B. Bourdon
    • 1
  1. 1.Institute of Geochemistry and PetrologyETH ZurichZurichSwitzerland
  2. 2.Institute of Biogeochemistry and Pollutant DynamicsETH ZurichZurichSwitzerland

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