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Biogeochemistry

, Volume 109, Issue 1–3, pp 271–285 | Cite as

Landscape-level nitrogen import and export in an ecosystem with complex terrain, Colorado Front Range

  • Anthony Darrouzet-Nardi
  • Joseph Erbland
  • William D. Bowman
  • Joel Savarino
  • Mark W. Williams
Article

Abstract

Knowledge of import, export, and transport of nitrogen (N) in headwater catchments is essential for understanding ecosystem function and water quality in mountain ecosystems, especially as these ecosystems experience increased anthropogenic N deposition. In this study, we link spatially explicit soil and stream data at the landscape scale to investigate import, export and transport of N in a 0.89 km2 site at the alpine-subalpine ecotone in the Front Range of the Rocky Mountains, Colorado, U.S.A. For two of the major N inputs to our site, N deposition in the snowpack and N fixation, a complementary relationship was found across the study site, with greater abundance of N-fixing plants in areas with less snow and substantial snow inputs in areas with low N fixer abundance. During the initial phases of snowmelt, mixing model end members for oxygen isotopes in nitrate (NO3 ) indicated that a substantial quantity of NO3 is transported downhill into the forested subalpine without being assimilated by soil microbes. After this initial pulse, much less NO3 entered the stream and most but not all of it was microbial in origin. Rising δ15N in stream NO3 indicated greater influence of fractionating processes such as denitrification later in the season. NO3 from both atmospheric and microbial sources was not exported from our site because it was consumed within the first several hundred meters of the stream; ultimately, N exports were in the form of dissolved organic nitrogen (DON) and particulate N (PN). The results of this study suggest that the highest elevation dry alpine meadows rely more heavily on N fixation as an N source and experience less of the effects of anthropogenic N deposition than mid and lower elevation areas that have more snow. Our data also suggest that mid-elevation krummholz, moist meadows, and talus slopes are exporting N as NO3 shortly after the onset of snowmelt, but that this NO3 is rapidly consumed as the stream flows through the subalpine forest. This consumption by assimilation and/or denitrification currently provides a buffer against increased inorganic N availability downstream.

Keywords

δ17δ18Δ17Kriging Landscape continuum LTER Niwot Ridge Dissolved organic nitrogen (DON) 

Notes

Acknowledgments

We thank NSF DGE 0202758 (Graduate Research Fellowship Program), NSF DEB 0423662 (Niwot Ridge LTER), the University of Colorado Ecology and Evolutionary Biology Department, and the John W. Marr Memorial Ecology Fund for funding this project. JS and JE thank the Institut National des Sciences de l’Univers (INSU) and its program LEFE-CHAT for the financial support provided for the analysis of the isotopic composition of nitrate. We thank Greg Michalski for his help with the isotopic measurements. For field help, we thank John Murgel, Carly Baroch, Brendan Whyte, Anna Lieb, Jaclyn Darrouzet-Nardi, Jeanette Darrouzet-Nardi, Chris Darrouzet-Nardi, Riley Graham, Andrea Dixon, and numerous volunteers that measured snow depths. For laboratory analyses, we thank Chris Seibold and the Kiowa Lab assistants. Logistical support was provided the University of Colorado’s Mountain Research Station.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Anthony Darrouzet-Nardi
    • 1
    • 2
    • 3
  • Joseph Erbland
    • 4
  • William D. Bowman
    • 1
    • 3
  • Joel Savarino
    • 4
  • Mark W. Williams
    • 3
    • 5
  1. 1.Department of Ecology and Evolutionary BiologyUniversity of ColoradoBoulderUSA
  2. 2.Department of Environmental SciencesUniversity of ToledoToledoUSA
  3. 3.Institute of Arctic and Alpine ResearchBoulderUSA
  4. 4.Laboratoire de Glaciologie et Géophysique de l’EnvironnementUniversité Joseph Fourier - Grenoble 1/CNRS-INSUSt.-Martin-d’HèresFrance
  5. 5.Department of GeographyUniversity of ColoradoBoulderUSA

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