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Biogeochemistry

, Volume 102, Issue 1–3, pp 209–222 | Cite as

Biological and photochemical transformations of amino acids and lignin phenols in riverine dissolved organic matter

  • Ronald BennerEmail author
  • Karl Kaiser
Article

Abstract

The photo- and bio-degradation of dissolved organic matter (DOM) in water from the Broad River were investigated in laboratory experiments using a solar simulator to control the intensity and exposure of samples to irradiation. The water samples included a natural assemblage of microorganisms, and the daily exposure of samples to irradiation was varied to distinguish the relative contributions of photochemical and biological degradation. Concentrations of dissolved organic carbon (DOC) and specific components of DOM, including chromophoric DOM (CDOM), lignin phenols and amino acids, were monitored to investigate the reactivity and predominant pathway of degradation of these DOM components. Biodegradation was primarily responsible for the overall remineralization of DOC and losses of the amino acid component of DOM, whereas photodegradation was primarily responsible for losses of the chromophoric and lignin phenol components of DOM. The rates of photodegradation of lignin phenols were strongly influenced by the presence of methoxy groups on the aryl ring. Syringyl (S) phenols have two methoxy substitutions, vanillyl (V) phenols have one methoxy substitution, and p-hydroxy (P) phenols are not substituted with methoxy groups. Photochemical decay constants were highest for S phenols, lowest for P phenols and followed a consistent pattern (S > V > P) in the experiments. The carbon-normalized yields of amino acids and lignin phenols were found to be useful molecular indicators of the highly reactive (i.e. labile) components of biodegradable and photodegradable DOM, respectively.

Keywords

Biodegradation Photodegradation DOM Amino acids Lignin phenols 

Notes

Acknowledgments

We thank Brian Benitez-Nelson for technical assistance with the experiments. This research was supported by NSF grant OCE 0850653.

References

  1. Alexander M (1965) Biodegradation: problems of molecular recalcitrance and microbial fallibility. Adv Appl Microbiol 7:35–80CrossRefGoogle Scholar
  2. Amon RMW, Benner R (1996) Photochemical and microbial consumption of dissolved organic carbon and dissolved oxygen in the Amazon River system. Geochim Cosmochim Acta 60:1783–1792CrossRefGoogle Scholar
  3. Amon RMW, Fitznar H-P, Benner R (2001) Linkages among the bioreactivity, chemical composition, and diagenetic state of marine dissolved organic matter. Limnol Oceanogr 46:287–297CrossRefGoogle Scholar
  4. Benner R (2003) Molecular indicators of the bioavailability of dissolved organic matter. In: Findlay SEG, Sinsabaugh RL (eds) Aquatic ecosystems: interactivity of dissolved organic matter. Academic Press, New York, pp 121–137Google Scholar
  5. Benner R, Biddanda B (1998) Photochemical transformations of surface and deep marine dissolved organic matter: effects on bacterial growth. Limnol Oceanogr 43:1373–1378CrossRefGoogle Scholar
  6. Benner R, Strom M (1993) A critical evaluation of the analytical blank associated with DOC measurements by high-temperature catalytic oxidation. Mar Chem 41:153–160CrossRefGoogle Scholar
  7. Benner R, Maccubbin AE, Hodson RE (1984) Anaerobic biodegradation of the lignin and polysaccharide components of lignocellulose and synthetic lignin by sediment microflora. Appl Environ Microbiol 47:998–1004 Google Scholar
  8. Benner R, Moran MA, Hodson RE (1985) Effects of pH and plant source on lignocellulose biodegradation rates in two wetland ecosystems, the Okefenokee Swamp and a Georgia salt marsh. Limnol Oceanogr 30:489-499CrossRefGoogle Scholar
  9. Benner R, Moran MA, Hodson RE (1986) Biogeochemical cycling of lignocellulosic carbon in marine and freshwater ecosystems: relative contributions of prokaryotes and eukaryotes. Limnol Oceanogr 31:99–100CrossRefGoogle Scholar
  10. Benner R, Fogel ML, Sprague KE, Hodson RE (1987) Depletion of 13C in lignin and its implications for stable carbon isotope studies. Nature 329:708–710CrossRefGoogle Scholar
  11. Davis J, Benner R (2005) Seasonal trends in the abundance, composition and bioavailability of particulate and dissolved organic matter in the Chukchi/Beaufort Seas and western Canada Basin. Deep Sea Res II 52:3396–3410CrossRefGoogle Scholar
  12. Davis J, Benner R (2007) Quantitative estimates of labile and semi-labile DOC in the western Arctic Ocean: a molecular approach. Limnol Oceanogr 52:2434–2444CrossRefGoogle Scholar
  13. Dickens AF, Gudeman JA, Gélinas Y, Baldock JA, Tinner W, Hu FS, Hedges JI (2007) Sources and distribution of CuO-derived benzene carboxylic acids in soils and sediments. Org Geochem 38:1256–1276CrossRefGoogle Scholar
  14. Druffel ERM, Williams PM, Bauer JE, Ertel JR (1992) Cycling of dissolved and particulate organic matter in the ocean. J Geophys Res 97:15639–15659CrossRefGoogle Scholar
  15. Hedges JI (1975) Lignin compounds as indicators of terrestrial organic matter in marine sediments. Ph.D. thesis, University of Texas at AustinGoogle Scholar
  16. Hedges JI, Parker PL (1976) Land-derived organic matter in surface sediments from the Gulf of Mexico. Geochim Cosmochim Acta 40:1019–1029CrossRefGoogle Scholar
  17. Hedges JI, Prahl FG (1993) Early diagenesis: consequences for applications of molecular biomarkers. In: Engel MH, Macko SA (eds) Organic geochemistry: principles and applications. Plenum Press, New York, pp 237–254Google Scholar
  18. Hedges JI, Clark WA, Cowie GL (1988) Organic matter sources to the water column and surficial sediments of a marine bay. Limnol Oceanogr 33:1116–1136CrossRefGoogle Scholar
  19. Hedges JI, Cowie GL, Richey JE, Quay PD, Benner R, Strom M (1994) Origins and processing of organic matter in the Amazon River as indicated by carbohydrates and amino acids. Limnol Oceanogr 39:743–761CrossRefGoogle Scholar
  20. Hedges JI, Keil RG, Benner R (1997) What happens to terrestrial organic matter in the ocean? Org Geochem 27:195–212CrossRefGoogle Scholar
  21. Hernes PJ, Benner R (2002) Transport and diagenesis of dissolved and particulate terrigenous organic matter in the North Pacific Ocean. Deep Sea Res I 49:2119–2132CrossRefGoogle Scholar
  22. Hernes PJ, Benner R (2003) Photochemical and microbial degradation of dissolved lignin phenols: implications for the fate of terrigenous dissolved organic matter in marine environments. J Geophys Res 108(C9). doi: 10.1029/2002JC001421
  23. Kaiser K, Benner R (2005) Hydrolysis-induced racemization of amino acids. Limnol Oceanogr Methods 3:318–325Google Scholar
  24. Louchouarn P, Opsahl S, Benner R (2000) Isolation and quantification of dissolved lignin from natural waters using solid-phase extraction (SPE) and GC/MS. Anal Chem 72:2780–2787CrossRefGoogle Scholar
  25. McNally AM, Moody EC, McNeill K (2005) Kinetics and mechanism of the sensitized photodegradation of lignin model compounds. Photochem Photobiol Sci 4:268–274CrossRefGoogle Scholar
  26. Miller WM, Moran MA (1997) Interaction of photochemical and microbial processes in the degradation of refractory dissolved organic matter from a coastal marine environment. Limnol Oceanogr 42:1317–1324CrossRefGoogle Scholar
  27. Miller WM, Zepp RG (1995) Photochemical production of dissolved inorganic carbon from terrestrial organic matter: significance to the oceanic organic carbon cycle. Geophys Res Lett 22:417–420CrossRefGoogle Scholar
  28. Mopper K, Kieber DJ (2002) Photochemistry and the cycling of carbon, sulfur, nitrogen and phosphorus. In: Hansell DA, Carlson CA (eds) Biogeochemistry of dissolved organic matter. Academic Press, San Diego, pp 455–507CrossRefGoogle Scholar
  29. Moran MA, Zepp RG (1997) Role of photoreactions in the formation of biologically labile compounds from dissolved organic matter. Limnol Oceanogr 42:1307–1316CrossRefGoogle Scholar
  30. Moran MA, Sheldon WM, Sheldon JE (1999) Biodegradation of riverine dissolved organic carbon in five estuaries of the southeastern United States. Estuaries 22:55–64CrossRefGoogle Scholar
  31. Obernosterer I, Benner R (2004) Competition between biological and photochemical processes in the mineralization of dissolved organic carbon. Limnol Oceanogr 49:117–124CrossRefGoogle Scholar
  32. Obernosterer I, Reitner B, Herndl GJ (1999) Contrasting effects of solar radiation on dissolved organic matter and its bioavailability to marine bacterioplankton. Limnol Oceanogr 44:1645–1654CrossRefGoogle Scholar
  33. Opsahl S, Benner R (1993) Decomposition dynamics of senescent blades of the seagrass Halodule wrightii Aschers in a subtropical lagoon. Mar Ecol Prog Ser 94:191–205CrossRefGoogle Scholar
  34. Opsahl S, Benner R (1997) Distribution and cycling of terrigenous dissolved organic matter in the ocean. Nature 386:480–482CrossRefGoogle Scholar
  35. Opsahl S, Benner R (1998) Photochemical reactivity of dissolved lignin in river and ocean waters. Limnol Oceanogr 43:1297–1304CrossRefGoogle Scholar
  36. Opsahl S, Zepp RG (2001) Photochemically induced alteration of stable carbon isotope ratios (δ13C) in terrigenous dissolved organic carbon. Geophys Res Lett 28:2417–2420CrossRefGoogle Scholar
  37. Osburn CL, Morris DP, Thorn KA, Moeller RE (2001) Chemical and optical changes in freshwater dissolved organic matter exposed to solar radiation. Biogeochemistry 54:251–278CrossRefGoogle Scholar
  38. Raymond PA, Bauer JE (2001) Use of 14C and 13C natural abundances for evaluating riverine, estuarine and coastal DOC and POC sources and cycling: a review and synthesis. Org Geochem 32:469–485CrossRefGoogle Scholar
  39. Reitner B, Herzig A, Herndl GJ (2001) Photoreactivity and bacterioplankton availability of aliphatic versus aromatic amino acids and a protein. Aquat Microb Ecol 26:305–311CrossRefGoogle Scholar
  40. Sarkanen KV, Ludwig CH (1971) Lignins: occurrence, formation, structure and reactions. Wiley-Interscience, New YorkGoogle Scholar
  41. Schmitt-Kopplin P, Hertkorn N, Schulten H-R, Kettrup A (1998) Structural changes in a dissolved soil humic acid during photochemical degradation processes under O2 and N2 atmosphere. Environ Sci Technol 32:2531–2541CrossRefGoogle Scholar
  42. Sondergaard M, Middelboe M (1995) A cross-system analysis of labile dissolved organic carbon. Mar Ecol Prog Ser 118:283–294CrossRefGoogle Scholar
  43. Spencer RGM, Stubbins A, Hernes PJ, Baker A, Mopper K, Aufdenkampe AK, Dyda RY, Mwamba VL, Magangu AM, Wabakanghanzi JN, Six J (2009) Photochemical degradation of dissolved organic matter and dissolved lignin phenols from the Congo River. J Geophys Res 114:G03010. doi: 10.1029/2009JG000968 CrossRefGoogle Scholar
  44. Stepanauskas R, Moran MA, Bergamaschi BA, Hollibaugh JT (2005) Sources, bioavailability, and photoreactivity of dissolved organic carbon in the Sacramento-San Joaquin River Delta. Biogeochemistry 74:131–149CrossRefGoogle Scholar
  45. Tranvik LJ, Bertilsson S (2001) Contrasting effects of solar UV radiation on dissolved organic sources for bacterial growth. Ecol Lett 4:458–463CrossRefGoogle Scholar
  46. Vodacek A, Blough NV, DeGrandpre MD, Peltzer ET, Nelson RK (1997) Seasonal variation of CDOM and DOC in the Middle Atlantic Bight: terrestrial inputs and photooxidation. Limnol Oceanogr 42:674–686CrossRefGoogle Scholar
  47. Volk CJ, Volk CB, Kaplan LA (1997) Chemical composition of biodegradable dissolved organic matter in streamwater. Limnol Oceanogr 42:39–44CrossRefGoogle Scholar
  48. Weiss M, Simon M (1999) Consumption of labile dissolved organic matter by limnetic bacterioplankton: the relative significance of amino acids and carbohydrates. Aquat Microb Ecol 17:1–12CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Biological Sciences and Marine Science ProgramUniversity of South CarolinaColumbiaUSA

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