, Volume 90, Issue 1, pp 65–73 | Cite as

Iron interference in the quantification of nitrate in soil extracts and its effect on hypothesized abiotic immobilization of nitrate

  • Eric A. Davidson
  • D. Bryan Dail
  • Jon Chorover
Original Paper


Human alteration of the nitrogen cycle has stimulated research on nitrogen cycling in many aquatic and terrestrial ecosystems, where analyses of nitrate (NO3 ) by standard laboratory methods are common. A recent study by Colman et al. (Biogeochemistry 84:161–169, 2007) identified a potential analytical interference of soluble iron (Fe) with NO3 quantification by standard flow-injection analysis of soil extracts, and suggested that this interference may have led Dail et al. (Biogeochemistry 54:131–146, 2001) to make an erroneous assessment of abiotic nitrate immobilization in prior 15N pool dilution studies of Harvard Forest soils. In this paper, we reproduce the Fe interference problem systematically and show that it is likely related to dissolved, complexed-Fe interfering with the colorimetric analysis of NO2 . We also show how standard additions of NO3 and NO2 to soil extracts at native dissolved Fe concentrations reveal when the Fe interference problem occurs, and permit the assessment of its significance for past, present, and future analyses. We demonstrate low soluble Fe concentrations and good recovery of standard additions of NO3 and NO2 in extracts of sterilized Harvard Forest soils. Hence, we maintain that rapid NO3 immobilization occurred in sterilized samples of the Harvard Forest O horizon in the study by Dail et al. (2001). Furthermore, additional evidence is accumulating in the literature for rapid disappearance of NO3 added to soils, suggesting that our observations were not the result of an isolated analytical artifact. The conditions for NO3 reduction are likely to be highly dependent on microsite properties, both in situ and in the laboratory. The so-called “ferrous wheel hypothesis” (Davidson et al., Glob Chang Biol 9:228–236, 2003) remains an unproven, viable explanation for published observations.


Iron Nitrate Nitrite Nitrogen Soil extracts Abiotic immobilization Ferrous wheel hypothesis 



BD would like to thank Bruce Hoskins and Krzysztof Lesniewicz of the Maine Agricultural and Forest Experiment Station Analytical Laboratory for many hours of discussion and analysis regarding N and Fe determinations. This research was supported by NSF grants DEB 0212505, DEB 0212675, and DEB 0212245.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Eric A. Davidson
    • 1
  • D. Bryan Dail
    • 2
  • Jon Chorover
    • 3
  1. 1.The Woods Hole Research CenterFalmouthUSA
  2. 2.Department of Plant, Soil, and Environmental SciencesUniversity of MaineOronoUSA
  3. 3.Department of Soil, Water and Environmental ScienceUniversity of ArizonaTucsonUSA

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