Biogeochemistry

, Volume 86, Issue 3, pp 301–318 | Cite as

Response of dissolved organic matter in the forest floor to long-term manipulation of litter and throughfall inputs

  • Karsten Kalbitz
  • Armin Meyer
  • Rong Yang
  • Pedro Gerstberger
Article

Abstract

Dissolved organic matter (DOM) contributes to organic carbon either stored in mineral soil horizons or exported to the hydrosphere. However, the main controls of DOM dynamics are still under debate. We studied fresh leaf litter and more decomposed organic material as the main sources of DOM exported from the forest floor of a mixed beech/oak forest in Germany. In the field we doubled and excluded aboveground litter input and doubled the input of throughfall. From 1999 to 2005 we measured concentrations and fluxes of dissolved organic C and N (DOC, DON) beneath the Oi and Oe/Oa horizon. DOM composition was traced by UV and fluorescence spectroscopy. In selected DOM samples we analyzed the concentrations of phenols, pentoses and hexoses, and lignin-derived phenols by CuO oxidation. DOC and DON concentrations and fluxes almost doubled instantaneously in both horizons of the forest floor by doubling the litter input and DOC concentrations averaged 82 mg C l−1 in the Oe/Oa horizon. Properties of DOM did not suggest a change of the main DOM source towards fresh litter. In turn, increasing ratios of hexoses to pentoses and a larger content of lignin-derived phenols in the Oe/Oa horizon of the Double litter plots in comparison to the Control plots indicated a priming effect: Addition of fresh litter stimulated microbial activity resulting in increased microbial production of DOM from organic material already stored in Oe/Oa horizons. Exclusion of litter input resulted in an immediate decrease in DOC concentrations and fluxes in the thin Oi horizon. In the Oe/Oa horizon DOC concentrations started to decline in the third year and were significantly smaller than those in the Control after 5 years. Properties of DOM indicated an increased proportion of microbially and throughfall derived compounds after exclusion of litter inputs. Dissolved organic N did not decrease upon litter exclusion. We assume a microbial transformation of mineral N from throughfall and N mineralization to DON. Increased amounts of throughfall resulted in almost equivalently increased DOC fluxes in the Oe/Oa horizon. However, long-term additional throughfall inputs resulted in significantly declining DOC concentrations over time. We conclude that DOM leaving the forest floor derives mainly from decomposed organic material stored in Oe/Oa horizons. Leaching of organic matter from fresh litter is of less importance. Observed effects of litter manipulations strongly depend on time and the stocks of organic matter in forest floor horizons. Long-term experiments are particularly necessary in soils/horizons with large stocks of organic matter and in studies focusing on effects of declined substrate availability. The expected increased primary production upon climate change with subsequently enhanced litter input may result in an increased production of DOM from organic soil horizons.

Keywords

Dissolved organic matter Field experiment Forest soil Hexoses Lignin Litter Pentoses Phenols 

Notes

Acknowledgements

We would like to thank Uwe Hell for help in the field and J.-H. Park for his work in the first 2 years of the experiment. Many colleagues and students of our department helped in sample preparation and analysis. We thank the members of the Central Analytical Department of BayCEER for support and Gunnar Lischeid for providing throughfall data. Egbert Matzner, Ludwig Haumaier, Klaus Kaiser, Thorsten Scheel, Thimo Klotzbücher, Susan Crow and Bruce Caldwell gave us valuable comments to an earlier version of this manuscript. We gratefully acknowledge the financial support by the German Ministry of Education and Research (BMBF) under grant No PT BEO 51-0339476.

References

  1. Amelung W, Flach KW, Zech W (1999) Lignin in particle-size fractions as influenced by climate. Soil Sci Soc Am J 63:1222–1228CrossRefGoogle Scholar
  2. Bahri H, Dignac MF, Rumpel C, Rasse DP, Chenu C, Mariotti A (2006) Lignin turnover kinetics in an agricultural soil is monomer specific. Soil Biol Biochem 38:1977–1988CrossRefGoogle Scholar
  3. Benner R, Weliky K, Hedges JI (1990) Early diagenesis of mangrove leaves in a tropical estuary: molecular-level analyses of neutral sugars and lignin-derived phenols. Geochim Cosmochim Acta 54:1991–2001CrossRefGoogle Scholar
  4. Bishop K, Seibert J, Köhler S, Laudon H (2004) Resolving the Double Paradox of rapidly mobilized old water with highly variable responses in runoff chemistry. Hydrol Process 18:185–189CrossRefGoogle Scholar
  5. Boone RD, Nadelhoffer KJ, Canary JD, Kaye JP (1998) Roots exert a strong influence on the temperature sensitivity of soil respiration. Nature 396:570–572CrossRefGoogle Scholar
  6. Bowden RD, Nadelhoffer KJ, Boone RD, Melillo JM, Garrison JB (1993) Contributions of aboveground litter, belowground litter, and root respiration to total soil respiration in a temperate mixed hardwood forest. Can J For Res 23:1402–1407CrossRefGoogle Scholar
  7. Brink RH Jr, Dubach P, Lynch DL (1960) Measurement of carbohydrates in soil hydrolyzates with anthrone. Soil Sci 89:157–166CrossRefGoogle Scholar
  8. Chantigny MH, Angers DA, Kaiser K, Kalbitz K (2007) Extraction and characterization of dissolved organic matter. In: Carter MR, Gregorich EG (eds) Soil sampling and methods of analysis, chap 48. CRC Press, pp 617–635Google Scholar
  9. Cleveland CC, Nemergut DR, Schmidt SK, Townsend AR (2007) Increases in soil respiration following labile carbon additions linked to rapid shifts in soil microbial community composition. Biogeochemistry 82:229–240CrossRefGoogle Scholar
  10. Currie WS, Aber JD (1997) Modeling leaching as a decomposition process in humid montane forests. Ecology 78:1844–1860CrossRefGoogle Scholar
  11. Dignac M-F, Bahri H, Rumpel C, Rasse DP, Bardoux G, Balesdent J, Girardin C, Chenu C, Mariotti A (2005) Carbon-13 natural abundance as a tool to study the dynamics of lignin monomers in soil: an appraisal at the Closeaux experimental field (France). Geoderma 128:3–17CrossRefGoogle Scholar
  12. Ertel JR, Hedges JI (1984) The lignin component of humic substances: Distribution among soil and sedimentary humic, fulvic, and base-insoluble fractions. Geochim Cosmochim Acta 48:2065–2074CrossRefGoogle Scholar
  13. Evans CD, Chapman PJ, Clark JM, Monteith DT, Cresser MS (2006) Alternative explanations for rising dissolved organic carbon export from organic soils. Global Change Biol 12:2044–2053CrossRefGoogle Scholar
  14. Findlay SEG (2005) Increased carbon transport in the Hudson River: unexpected consequence of nitrogen deposition? Front Ecol Environ 3:133–137CrossRefGoogle Scholar
  15. Fröberg M, Berggren D, Bergkvist B, Bryant C, Knicker H (2003) Contributions of Oi, Oe and Oa horizons to dissolved organic matter in forest floor leachates. Geoderma 113:311–322CrossRefGoogle Scholar
  16. Fröberg M, Berggren Kleja D, Bergkvist B, Tipping E, Mulder J (2005) Dissolved organic carbon leaching from a coniferous forest floor—a field manipulation experiment. Biogeochemistry 75:271–287CrossRefGoogle Scholar
  17. Fröberg M, Berggren Kleja D, Hagedorn F (2007) The contribution of fresh litter to dissolved organic carbon leached from a coniferous forest floor. Eur J Soil Sci 58:10–114CrossRefGoogle Scholar
  18. Gerstberger P, Foken T, Kalbitz K (2004) The Lehstenbach and Steinkreuz catchments in NE Bavaria, Germany. In: Matzner E (ed) Biogeochemistry of forested catchments in a changing environment: a German case study. Ecol Stud, vol 172. Springer Verlag, Berlin Heidelberg, pp 15–44Google Scholar
  19. Goñi MA, Nelson B, Blanchette RA, Hedges JI (1993) Fungal degradation of wood lignins: geochemical perspectives from CuO-derived phenolic dimers and monomers. Geochim Cosmochim Acta 57:3985–4002CrossRefGoogle Scholar
  20. Guggenberger G, Zech W (1994) Composition and dynamics of dissolved carbohydrates and lignin-degradation products in two coniferous forests, N.E. Bavaria, Germany. Soil Biol Biochem 26:19–27CrossRefGoogle Scholar
  21. Guggenberger G, Christensen BT, Zech W (1994) Land-use effects on the composition of organic matter in particle size separates of soil: I. Lignin and carbohydrate signature. Eur J Soil Sci 45:449–458CrossRefGoogle Scholar
  22. Hagedorn F, Saurer M, Blaser P (2004) A C-13 tracer study to identify the origin of dissolved organic carbon in forested mineral soils. Eur J Soil Sci 55:91–100CrossRefGoogle Scholar
  23. Haider KM, Guggenberger G (2005) Organic matter. Genesis and formation. In: Hillel D (ed) Encyclopedia of soils in the environment, vol 3. Elsevier, pp 93–101Google Scholar
  24. Hedges JI, Ertel JR (1982) Characterization of lignin by gas capillary chromatography of cupric oxide oxidation products. Anal Chem 54:174–178CrossRefGoogle Scholar
  25. Huang WZ, Schoenau JJ (1996) Distribution of water-soluble organic carbon in an aspen forest soil. Can J For Res 26:1266–1272Google Scholar
  26. Huang JH, Kalbitz K, Matzner E (2008) Mobility of trimethyllead and total lead in the forest floor. Soil Sci Soc Am J (in press)Google Scholar
  27. Kaiser K, Guggenberger G, Haumaier L (2004) Changes in dissolved lignin-derived phenols, neutral sugars, uronic acids, and amino sugars with depth in forested Haplic Arenosols and Rendzic Leptosols. Biogeochemistry 70:135–151CrossRefGoogle Scholar
  28. Kalbitz K, Geyer W (2001) Humification indices of water-soluble fulvic acids derived from synchronous fluorescence spectra—effects of spectrometer type and concentration. J Plant Nutr Soil Sci 164:259–265CrossRefGoogle Scholar
  29. Kalbitz K, Solinger S, Park JH, Michalzik B, Matzner E (2000) Controls on the dynamics of dissolved organic matter in soils: a review. Soil Sci 165:277–304CrossRefGoogle Scholar
  30. Kalbitz K, Schmerwitz J, Schwesig D, Matzner E (2003a) Biodegradation of soil-derived dissolved organic matter as related to its properties. Geoderma 113:273–291CrossRefGoogle Scholar
  31. Kalbitz K, Schwesig D, Schmerwitz J, Kaiser K, Haumaier L, Glaser B, Ellerbrock R, Leinweber P (2003b) Changes in properties of soil-derived dissolved organic matter induced by biodegradation. Soil Biol Biochem 35:1129–1142CrossRefGoogle Scholar
  32. Kalbitz K, Glaser B, Bol R (2004) Clear-cutting of a Norway spruce stand: implications for controls on the dynamics of dissolved organic matter in the forest floor. Eur J Soil Sci 55:401–413CrossRefGoogle Scholar
  33. Kalbitz K, Schwesig D, Rethemeyer J, Matzner E (2005) Stabilization of dissolved organic matter by sorption to the mineral soil. Soil Biol Biochem 37:1319–1331CrossRefGoogle Scholar
  34. Kalbitz K, Kaiser K, Bargholz J, Dardenne P (2006) Lignin degradation controls the production of dissolved organic matter in decomposing foliar litter. Eur J Soil Sci 57:504–516CrossRefGoogle Scholar
  35. Kiikkilä O, Kitunen V, Smolander A (2006) Dissolved soil organic matter from surface organic horizons under birch and conifers: degradation in relation to chemical characteristics. Soil Biol Biochem 38:737–746CrossRefGoogle Scholar
  36. Kögel I (1986) Estimation and decomposition pattern of the lignin component in forest humus layers. Soil Biol Biochem 18:589–594CrossRefGoogle Scholar
  37. Kögel-Knabner I, Zech W, Hatcher PG (1988) Chemical composition of the organic matter in forest soils: the humus layer. Z Pflanzenernaehr Bodenkd 151:331–340CrossRefGoogle Scholar
  38. Lajtha K, Crow SE, Yano Y, Kaushal SS, Sulzman E, Sollins P, Spears JDH (2005) Detrital controls on soil solution N and dissolved organic matter in soils: a field experiment. Biogeochemistry 76:261–281CrossRefGoogle Scholar
  39. McDowell WH (2003) Dissolved organic matter in soils—future directions and unanswered questions. Geoderma 113:179–186CrossRefGoogle Scholar
  40. McKnight DM, Harnish R, Wershaw RL, Baron JS, Schiff S (1997) Chemical characteristics of particulate, colloidal, and dissolved organic material in Loch Vale Watershed, Rocky Mountain National Park. Biogeochemistry 36:99–124CrossRefGoogle Scholar
  41. Mejbaum W (1939) Über die Bestimmung kleiner Pentosemengen, insbesondere in Derivaten der Adenylsäure. Z Physiol Chemie 258:117–120Google Scholar
  42. Michalzik B, Matzner E (1999) Dynamics of dissolved organic nitrogen and carbon in a Central European Norway spruce ecosystem. Eur J Soil Sci 50:579–590CrossRefGoogle Scholar
  43. Michalzik B, Kalbitz K, Park JH, Solinger S, Matzner E (2001) Fluxes and concentrations of dissolved organic carbon and nitrogen—a synthesis for temperate forests. Biogeochemistry 52:173–205CrossRefGoogle Scholar
  44. Michalzik B, Tipping E, Mulder J, Gallardo Lancho JF, Matzner E, Bryant CL, Clarke N, Lofts S, Vicente Esteban MA (2003) Modelling the production and transport of dissolved organic carbon in forest soils. Biogeochemistry 66:241–264CrossRefGoogle Scholar
  45. Morales P, Hickler T, Rowell DP, Smith B, Sykes MT (2007) Changes in European ecosystem productivity and carbon balance driven by regional climate model output. Global Change Biol 13:108–122CrossRefGoogle Scholar
  46. Neff JC, Asner G (2001) Dissolved organic carbon in terrestrial ecosystems: Synthesis and a model. Ecosystems 4:29–48CrossRefGoogle Scholar
  47. Oades JM (1984) Soil organic matter and structural stability: mechanisms and implications for management. Plant Soil 76:319–337CrossRefGoogle Scholar
  48. Park J-H, Matzner E (2003) Controls on the release of dissolved organic carbon and nitrogen from a deciduous forest floor investigated by manipulations of aboveground litter inputs and water flux. Biogeochemistry 66:265–286CrossRefGoogle Scholar
  49. Park JH, Kalbitz K, Matzner E (2002) Resource control on the production of dissolved organic carbon and nitrogen in a deciduous forest floor. Soil Biol Biochem 34:813–822CrossRefGoogle Scholar
  50. Qualls RG, Haines BL, Swank WT (1991) Fluxes of dissolved organic nutrients and humic substances in a deciduous forest. Ecology 72:254–266CrossRefGoogle Scholar
  51. Roulet N, Moore TR (2006) Browning the waters. Nature 444:283–284CrossRefGoogle Scholar
  52. Rumpel C, Kögel-Knabner I, Bruhn F (2002) Vertical distribution, age, and chemical composition of organic, carbon in two forest soils of different pedogenesis. Org Geochem 33:1131–1142CrossRefGoogle Scholar
  53. Scheel T, Dörfler C, Kalbitz K (2007) Precipitation of dissolved organic matter by Al stabilizes carbon in acidic forest soils. Soil Sci Soc Am J 71:64–74Google Scholar
  54. Seely B, Lajtha K (1997) Application of a N-15 tracer to simulate and track the fate of atmospherically deposited N in the coastal forests of the Waquoit Bay Watershed, Cape Cod, Massachusetts. Oecologia 112:393–402CrossRefGoogle Scholar
  55. Solinger S, Kalbitz K, Matzner E (2001) Controls on the dynamics of dissolved organic carbon and nitrogen in a Central European deciduous forest. Biogeochemistry 55:327–349CrossRefGoogle Scholar
  56. Sulzman EW, Brant JB, Bowden RD, Lajtha K (2005) Contribution of aboveground litter, belowground litter, and rhizosphere respiration to total soil CO2 efflux in an old growth coniferous forest. Biogeochemistry 73:231–256CrossRefGoogle Scholar
  57. Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica. I.—The quantitative analysis of phenolic constituents. J Sci Food Agricul 10:63–68CrossRefGoogle Scholar
  58. Tipping E, Woof C, Rigg E, Harrison AF, Ineson P, Taylor K, Benham D, Poskitt J, Rowland AP, Bol R, Harkness DD (1999) Climatic influences on the leaching of dissolved organic matter from upland UK moorland soils, investigated by a field manipulation experiment. Environ Int 25:83–95CrossRefGoogle Scholar
  59. Traina S, Novak J, Smeck NE (1990) An ultraviolet absorbance method of estimating the percent aromatic carbon content of humic acids. J Environ Qual 19:151–153CrossRefGoogle Scholar
  60. WRB (2006) World reference base for soil resources 2006. World Soil Resources Reports 103, FAO, RomeGoogle Scholar
  61. Yano Y, Lajtha K, Sollins P, Caldwell BA (2004) Chemical and seasonal controls on the dynamics of dissolved organic matter in a coniferous old-growth stand in the Pacific Northwest, USA. Biogeochemistry 71:197–223CrossRefGoogle Scholar
  62. Zsolnay A (2003) Dissolved organic matter: artefacts, definitions and functions. Geoderma 113:187–209CrossRefGoogle Scholar
  63. Zsolnay A, Baigar E, Jimenez M, Steinweg B, Saccomandi F (1999) Differentiating with fluorescence spectroscopy the sources of dissolved organic matter in soils subjected to drying. Chemosphere 38:45–50CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Karsten Kalbitz
    • 1
  • Armin Meyer
    • 1
    • 2
  • Rong Yang
    • 3
  • Pedro Gerstberger
    • 4
  1. 1.Department of Soil EcologyUniversity of BayreuthBayreuthGermany
  2. 2.Institute of Groundwater EcologyGSF—National Research Center for Environment and HealthNeuherbergGermany
  3. 3.College of Resource & Environmental SciencesNorthwest A&F UniversityYanglingChina
  4. 4.Department of Plant EcologyUniversity of BayreuthBayreuthGermany

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