, Volume 73, Issue 3, pp 499–513

Denitrification and N2O emission from forested and cultivated alluvial clay soil



Restored forested wetlands reduce N loads in surface discharge through plant uptake and denitrification. While removal of reactive N reduces impact on receiving waters, it is unclear whether enhanced denitrification also enhances emissions of the greenhouse gas N2O, thus compromising the water-quality benefits of restoration. This study compares denitrification rates and N2O:N2 emission ratios from Sharkey clay soil in a mature bottomland forest to those from an adjacent cultivated site in the Lower Mississippi Alluvial Valley. Potential denitrification of forested soil was 2.4 times of cultivated soil. Using intact soil cores, denitrification rates of forested soil were 5.2, 6.6 and 2.0 times those of cultivated soil at 70, 85 and 100% water-filled pore space (WFPS), respectively. When NO3 was added, N2O emissions from forested soil were 2.2 times those of cultivated soil at 70% WFPS. At 85 and 100% WFPS, N2O emissions were not significantly different despite much greater denitrification rates in the forested soil because N2O:N2 emission ratios declined more rapidly in forested soil as WFPS increased. These findings suggest that restoration of forested wetlands to reduce NO3 in surface discharge will not contribute significantly to the atmospheric burden of N2O.


Bottomland hardwood Denitrification Global climate change Greenhouse gas Nitrous Oxide Sharkey soil Wetland restoration 



Lower Mississippi Alluvial Valley


potential denitrification assay


water-filled pore space


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  1. 1.
    Allen, J.A., Keeland, B.D., Stauturf, J.A., Clewell, A.F., Kennedy, H.E. 2001A guide to bottomland hardwood restorationUSGS/BRD/ITR-2000-0011132Technical ReportGoogle Scholar
  2. 2.
    Baggs, E.M., Rees, R.M., Castle, K., Scott, A., Smith, K.A., Vinten, A.J.A. 2002Nitrous oxide release from soils receiving N-rich crop residues and paper mill sludge in eastern ScotlandAgric. Ecosyst. Environ.90109123CrossRefGoogle Scholar
  3. 3.
    Blackmer, A.M., Bremner, J.M., Schmidt, E.L. 1980Production of nitrous oxide by ammonia-oxidizing chemiautotrophic microorganisms in soilAppl. Environ. Microbiol.4010601066Google Scholar
  4. 4.
    Breitenbeck, G.A., Blackmer, A.M., Bremner, J.M. 1980Effects of different nitrogen fertilizers on emissions of nitrous oxide from soilsGeophys. Res. Lett.78588Google Scholar
  5. 5.
    Burford, J.R., Bremner, J.M. 1975Relationship between the denitrification capacities of soils and total, water-soluble and readily decomposable soil organic matterSoil Biol. Biochem.7389394CrossRefGoogle Scholar
  6. 6.
    Dalal, R.C., Wang, W., Robertson, G.P., Parton, W.J. 2003Nitrous oxide emission from Australian agricultural lands and mitigationAust. J. Soil Res.41165195CrossRefGoogle Scholar
  7. 7.
    Fisk N.H. 1951. Mississippi River geology relation to river regime. American Society of Civil Engineers Transactions. Paper no: 2511, pp. 667–682.Google Scholar
  8. 8a.
    Gaskell, J.F., Blackmer, A.M., Bremner, J.M. 1980Comparison of the effects of nitratenitrite and nitric oxide on the reduction of nitrous oxide to dinitrogen by soil microorganismsSoil Sci. Soc. Am. J.4511241127Google Scholar
  9. 8.
    Granli, T., Bockman, O.C. 1994Nitrous oxide emissions from agriculture NorwayJ. Agric. Sci. Suppl.12Google Scholar
  10. 9.
    Groffman, P.M., Tiedje, J.M. 1988Denitrification hysteresis during wetting and drying cycles in soilSoil Sci. Soc. Am. J.5216261629Google Scholar
  11. 10.
    Hefting, M.M., Bobbink, R., de Caluwe, H. 2003Nitrous oxide emissions and denitrification in chronically nitrated-loaded riparian buffer zonesJ. Environ. Qual.3211941203PubMedGoogle Scholar
  12. 11.
    Hunter, R.G., Faulkner, S.P. 2001Denitrification potential in restored and natural hardwood wetlandsSoil Sci. Soc. Am. J.6518651872Google Scholar
  13. 12.
    Khalil, M.I., Rosenani, A.B., Van Cleemput, O., Fauziah, C.I., Shamshuddin, J. 2002Nitrous oxide emissions from an ultisol of the humid tropics under Maize-Groundnut rotationJ. Environ. Qual.3110711078PubMedGoogle Scholar
  14. 13.
    Klein De, C.A.M., Van Logtestijn, R.S.P. 1996Denitrification in grassland soils in the Netherlands in relation to irrigation, N-application ratesoil water content and soil temperatureSoil Biol. Biochem.28231237CrossRefGoogle Scholar
  15. 14.
    Linn, D.M., Doran, J.W. 1984Effect of water-filled pore space on carbon dioxide and nitrous oxide production in tilled and non-tilled soilsSoil Sci. Soc. Am. J.4812671272Google Scholar
  16. 15.
    MacDonald, P.O., Frayer, W.E., Clauser, J.K. 1979Documentation, chronology and future projections of bottomland hardwood habitat losses in the lower Mississippi alluvial plainUSFWSWashington, DC427Google Scholar
  17. 16.
    Mahdun, B.Y., Young, J.L., Freed, V.H. 1986Binding of herbicides by water soluble organic materials from soilJ. Environ. Qual.156468Google Scholar
  18. 17.
    Mitsch W.J., Day J.W. Jr. Gilliam J.W., Groffman P.M., Hey D.L., Randall G.W. and Wang N. 1999. Reducing nutrient loads, especially nitrate-nitrogen, so surface watergroundwater and the Gulf of Mexico: Topic 5 Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico. NOAA Coastal Ocean OfficeSilver Spring, MD, Decision Analysis Series No. 19.Google Scholar
  19. 18.
    Mitsch, W.J., Day, Jr. J.W, Gilliam, J.W., Groffman, P.M., Hey, D.L., Randall, G.W., Wang, N. 2001Reducing nitrogen loading to the Gulf of Mexico from the Mississippi River basin: strategies to counter a persistent ecological problemBioScience51373388Google Scholar
  20. 19.
    Mosier, A.R., Hutchison, G.L. 1981Nitrous oxide emissions from cropped fieldsJ. Environ. Qual.10169173Google Scholar
  21. 20.
    NRCS1959Soil Survey-Sunflower County, MississippiSeries No. 5Natural Resource Conservation Service and Mississippi Agricultural Experiment StationMississippiGoogle Scholar
  22. 21.
    Sahrawat, K.L., Keeney, D.R. 1986

    Nitrous oxide emission from soils

    Advances in Soil ScienceSpringer-Verlag, Inc.New York103143
    Google Scholar
  23. 22.
    Sanders, D.L., Kalff, J. 2001Nitrogen retention in wetlands, lakes and riversHydrobiologia443205212CrossRefGoogle Scholar
  24. 23.
    SAS, Inc.1998SAS User Manual and SoftwareSAS Inc.North CarolinaUSGoogle Scholar
  25. 24.
    Sexstone, A.J., Parkin, T.B., Tiedje, J.M. 1985Temporal response of soil denitrification rates to rainfall and irrigationSoil Sci. Soc. Am. J.4999103Google Scholar
  26. 25.
    Sexstone, A.J., Parkin, T.B., Tiedje, J.M. 1988Denitrification response to soil wetting in aggregated and unaggregated soilSoil Biol. Biochem.20767769CrossRefGoogle Scholar
  27. 26.
    Simek, M., Elhottova, D., Klimes, F., Hopkins, D.W. 2004Emissions of N2O and CO2denitrification measurements and soil properties in red clover and ryegrass standsSoil Biol. Biochem.36921CrossRefGoogle Scholar
  28. 27.
    Singh, B., Ryden, J.C., Whitehead, D.C. 1988Some relationships between denitrification potential and fractions of organic carbon in air-dried and field moist soilsSoil Biol. Biochem.20737741CrossRefGoogle Scholar
  29. 28.
    Tiedje, J.M. 1982


    Page, A.L. eds. Methods of Soil Analysis, 2nd ed., Agronomy MonographAmerican Society of AgronomyMadison, WIVol. 9
    Google Scholar
  30. 29.
    Tilsner, J., Wrage, N., Lauf, J., Gebauer, G. 2003Emission of gaseous nitrogen oxides from extensively managed grassland in NE BavariaGermany. 1. Annual budget of N2O and NOx emissionsBiogeochemistry63229247CrossRefGoogle Scholar
  31. 30.
    Turner, D.P., Koerper, G.J., Harmon, M.E., Lee, J.J. 1995A carbon budget for forests of the conterminous United StatesEcol. Appl.5421436Google Scholar
  32. 31.
    Turner, R.E. 1998Fluctuating silicate: nitrate ratios and coastal plankton food websProc. Natl. Acad. Sci. USA951304813051CrossRefPubMedGoogle Scholar
  33. 32.
    Vinther, P.F. 1984Total denitrification and the ratio between N2O and N2 during the growth of spring barleyPlant Soil76227232Google Scholar
  34. 33.
    Walker, J.T., Geron, C.D., Vose, J.M., Swank, W.T. 2002Nitrogen trace gas emissions from a riparian ecosystem in southern AppalachiaChemosphere4913891398CrossRefPubMedGoogle Scholar
  35. 34.
    Weier, K.L., Doran, J.W., Power, J.F., Walters, D.T. 1993Denitrification and the dinitrogen/nitrous oxide ratio as affected by soil wateravailable carbon and nitrateSoil Sci. Soc. Am. J.576672Google Scholar
  36. 35.
    Weitz, A.M., Linder, E., Frolking, S., Crill, P.M., Keller, M. 2001N2O emissions from humid tropical agricultural soils: effects of soil moisturetexture and nitrogen availabilitySoil Biol. Biochem.3310771093CrossRefGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Wetlands Biogeochemistry InstituteLouisiana State UniversityBaton RougeUSA
  2. 2.Department of Agronomy and Environmental ManagementLouisiana State UniversityBaton RougeUSA
  3. 3.USGS National Wetlands Research CenterLafayetteUSA

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