Bio-emulsifying and biodegradation activities of syringafactin producing Pseudomonas spp. strains isolated from oil contaminated soils
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Pseudomonas strains isolated from oil contaminated soils were screened for biosurfactant production. Three out of eleven Pseudomonas isolates were selected for their high emulsifying activity (E24 value on n-hexadecane ~ 78%). These isolates (E39, E311 and E313) were identified as members of the P. putida group using phenotypical methods and a molecular approach. To identify the chemical nature of produced biosurfactants, thin layer chromatography and MALDI-ToF mass spectrometry analysis were carried out and revealed lipopeptides belonging to the syringafactin family. The activity of the produced biosurfactants was stable over a pH range of 6–12, at high salinity (10%) and after heating at 80 °C. Tests in contaminated sand micro-bioreactors showed that the three strains were able to degrade diesel. These results suggest the potential of these syringafactin producing strains for application in hydrocarbon bioremediation.
KeywordsPseudomonas Biosurfactant Bioemulsifier Biodegradation Lipopeptide
This work was funded by the Ministry of Higher Education and Scientific Research of Tunisia and a scholarship from the region Hauts-de-France. This work was supported by the European Union SMARTBIOCONTROLPORTFOLIO-Interreg FWVLBIOPROD program, the FEDER program ALIBIOTECH and the CPER project ALIBIOTECH from the region Hauts-de-France. The authors are grateful to the Advanced High Throughput Technologies Platform for Biorefineries Catalysts Design ‘REALCAT’ for allowing the implementation of MALDI-ToF mass spectrometry analysis. The authors are grateful to Kamel Maaloul, translator and English professor for having proofread the manuscript.
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