Characteristics and metabolic pathway of acetamiprid biodegradation by Fusarium sp. strain CS-3 isolated from soil
- 126 Downloads
An acetamiprid-degrading fungus was isolated from contaminated soil and identified as Fusarium sp. strain CS-3 based on physiological, biochemical, and molecular analyses. Strain CS-3 exploited 50 mg/L as the sole carbon source in liquid culture, removing 98% in 96 h. Strain CS-3 retained its acetamiprid degradation abilities over a wide range of pH (5.0–8.0) and temperature (20–42 °C). HPLC–MS analysis showed that N′-[(6-chloropyridin-3-yl)methyl]-N-methylacetamide, 2-chloro-5-hydroxymethylpyridine, and 6-chloronicotinic acid were identified as the most predominant metabolites, forming the basis for a newly described acetamiprid degradation pathway. Strain CS-3 efficiently degraded 99.6% of 50 mg/kg acetamiprid in soil, indicating potential for soil remediation.
KeywordsAcetamiprid 6-Chloronicotinic acid Fusarium sp. Strain CS-3 Acetamiprid-contaminated soil remediation
This work was supported by the National Natural Science Foundation of China (No. 31700092, No. 21727818, No. 21390200, No. 21706125, No. 21706124), the Jiangsu Province Natural Science Foundation for Youths (No. BK20170997, No. BK20170993), The Key Science and Technology Project of Jiangsu Province (No. BE2016389), The Project of State Key Laboratory of Materials Oriented Chemical Engineering (KL17-09), and The Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTE1834).
- Ahuactzin-Pérez M, Tlecuitl-Beristain S, García-Dávila J, González-Pérez M, Gutiérrez-Ruíz MC, Sánchez C (2016) Degradation of di(2-ethyl hexyl) phthalate by Fusarium culmorum: kinetics, enzymatic activities and biodegradation pathway based on quantum chemical modeling pathway based on quantum chemical modeling. Sci Total Environ 566:1186–1193CrossRefGoogle Scholar
- Anasontzis GE, Zerva A, Stathopoulou PM, Haralampidis K, Diallinas G, Karagouni AD, Hatzinikolaou DG (2011) Homologous overexpression of xylanase in Fusarium oxysporum increases ethanol productivity during consolidated bioprocessing (CBP) of lignocellulosics. J Biotechnol 152:16–23CrossRefGoogle Scholar
- Dong W, Chen Q, Hou Y, Li S, Zhuang K, Huang F, Zhou J, Li Z, Wang J, Fu L, Zhang Z, Huang Y, Wang F, Cui Z (2015) Metabolic pathway involved in 2-methyl-6-ethylaniline degradation by Sphingobium sp. strain MEA3-1 and the cloning of a novel flavin-dependent monooxygenase system meaBA. Appl Environ Microbiol 81(24):8254–8264CrossRefGoogle Scholar
- Fan H, Zhou W, Wang X, Luo Z, He F (2013) Biological characteristics of Fusarium oxysporum f. sp. niveum isolated from watermelon cultivated in plastic greenhouse and indoor toxicity determination of different fungicides in Hainan province of China. Plant Dis Pests 42(5):1146–1149Google Scholar
- Senyildiz M, Kilinc A, Ozden S (2016) Effects of neonicotinoid insecticides in cytotoxicity and DNA damage in HepG2 and SH-SY5Y cells. Toxicol Lett 258:S237–S238Google Scholar
- Zhou L, Zhang L, Sun S, Ge F, Mao S, Ma Y, Liu Z, Dai Y, Yuan S (2014) Degradation of the neonicotinoid insecticide acetamiprid via the N-carbamoylimine derivate (IM-1-2) mediated by the nitrile hydratase of the nitrogen-fixing bacterium Ensifer meliloti CGMCC 7333. J Agric Food Chem 62(41):9957–9964CrossRefGoogle Scholar