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Growth of Dehalococcoides mccartyi species in an autotrophic consortium producing limited acetate

  • Chang Ding
  • Lisa Alvarez-Cohen
  • Jianzhong He
Original Paper
  • 13 Downloads

Abstract

The dechlorinating Dehalococcoides mccartyi species requires acetate as carbon source, but little is known on its growth under acetate limiting conditions. In this study, we observed growth and dechlorination of a D. mccartyi-containing mixed consortium in a fixed-carbon-free medium with trichloroethene in the aqueous phase and H2/CO2 in the headspace. Around 4 mM formate was produced by day 40, while acetate was constantly below 0.05 mM. Microbial community analysis of the consortium revealed dominance by D. mccartyi and Desulfovibrio sp. (57 and 22% 16S rRNA gene copies, respectively). From this consortium, Desulfovibrio sp. strain F1 was isolated and found to produce formate and acetate (1.2 mM and 48 µM, respectively, by day 24) when cultivated alone in the above mentioned medium without trichloroethene. An established co-culture of strain F1 and D. mccartyi strain 195 demonstrated that strain 195 could grow and dechlorinate using acetate produced by strain F1; and that acetate was constantly below 25 µM in the co-culture. To verify that such low level of acetate is utilizable by D. mccartyi, we cultivated strain 195 alone under acetate-limiting conditions and found that strain 195 consumed acetate to below detection (5 µM). Based on the acetate consumption and cell yield of D. mccartyi, we estimated that on average 1.2 × 108 acetate molecules are needed to supply carbon for one D. mccartyi cell. Our study suggests that Desulfovibrio may supply a steady but low amount of fixed carbon to dechlorinating bacteria, exhibiting important implications for natural bio-attenuation when fixed carbon is limited.

Keywords

Dehalococcoides Organohalide respiration Chlorinated ethenes Bioremediation Autotrophic growth Acetate Desulfovibrio 

Notes

Acknowledgements

This work was supported by Ng Teng Fong Charitable Foundation (NTFCF) Fund [Grant Number R302-000-198-720] and the National Research Foundation, Prime Minister’s Office, Singapore under the Competitive Research Programme [Grant Number NRF-CRP5-2009-05]. We thank Professor Stephen H. Zinder at Cornell University for providing D. mccartyi strain 195.

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interest.

Supplementary material

10532_2018_9846_MOESM1_ESM.docx (720 kb)
Supplementary material 1 (DOCX 719 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Civil and Environmental EngineeringNational University of SingaporeSingaporeSingapore
  2. 2.Department of Civil and Environmental EngineeringUniversity of CaliforniaBerkeleyUSA
  3. 3.Earth Sciences DivisionLawrence Berkeley National LaboratoryBerkeleyUSA
  4. 4.Department of Isotope BiogeochemistryHelmholtz-Centre for Environmental Research - UFZLeipzigGermany
  5. 5.Department of Civil and Environmental EngineeringNational University of SingaporeSingaporeSingapore

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