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Biodegradation

, Volume 29, Issue 1, pp 71–88 | Cite as

Whole community transcriptome of a sequencing batch reactor transforming 2,4-dinitroanisole (DNAN) and 3-nitro-1,2,4-triazol-5-one (NTO)

  • Jennifer WeidhaasEmail author
  • Alexander Panaccione
  • Ananda Shankar Bhattacharjee
  • Ramesh Goel
  • Angela Anderson
  • Saraswati Poudel Acharya
Original Paper

Abstract

Two sequencing batch reactors (SBRs) were run to bio-mineralize 2,4-dinitroanisole (DNAN) and 3-nitro-1,2,4-triazol-5-one (NTO) in lab scale settings. The reactors were shown to reproducibly biotransform these munitions under aerobic and anaerobic conditions during the operations of these SBRs. Complete removal (100% biotransformation) of DNAN (initially 17.7 ± 5.4 mg L−1) and NTO (initially 15.0 ± 7.1 mg L−1) was observed in an anaerobic SBR when Luria-Bertani (LB) broth was present. In contrast, an aerobic SBR degraded only 58 ± 22% of DNAN (initially 19.7 ± 6.2 mg L−1) and 45 ± 24% of NTO (initially 9.7 ± 6.3 mg L−1) when either LB or glucose was also added indicating that anaerobic conditions are more favorable for biotransformation of these munitions. Transcriptomic analysis of the DNAN and NTO degrading anaerobic SBR revealed upregulation of a putative nitroreductase, hydroxylaminophenol mutases, 4-hydroxylphenyl acetate associated genes, and quinone oxioreductases. Major Bacterial populations included Bacteroidales, Campylobacterales, Enterobacteriales, Pseudomonadales, Burkholderiales and Clostridiales. Results from this study can be used to inform investigation of munition degrading organisms and the functional genes responsible.

Keywords

Munitions Biotransformation Sequencing batch reactor Transcriptomics 

Notes

Acknowledgements

Partial funding for this work was obtained from the West Virginia University Civil and Environmental Engineering Summer Undergraduate Research Fellowship program. The authors would like to acknowledge the Genomics Core Facility at West Virginia University for their support with Next Generation library prep and sequencing.

Supplementary material

10532_2017_9814_MOESM1_ESM.xls (27.6 mb)
Supplementary material 1 (XLS 28310 kb)
10532_2017_9814_MOESM2_ESM.docx (134 kb)
Supplementary material 2 (DOCX 133 kb)

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Authors and Affiliations

  1. 1.West Virginia University, Civil and Environmental EngineeringMorgantownUSA
  2. 2.University of Utah, Civil and Environmental EngineeringSalt Lake CityUSA
  3. 3.Bigelow Laboratory for Ocean SciencesEast BoothbayUSA

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