Nitrogen removal performance of anaerobic ammonia oxidation (ANAMMOX) in presence of organic matter
A sequencing batch reactor (SBR) was used to test the nitrogen removal performance of anaerobic ammonium oxidation (ANAMMOX) in presence of organic matter. Mesophilic operation (30 ± 0.5 °C) was performed with influent pH 7.5. The results showed, independent of organic matter species, ANAMMOX reaction was promoted when COD was lower than 80 mg/L. However, specific ANAMMOX activity decreased with increasing organic matter content. Ammonium removal efficiency decreased to 80% when COD of sodium succinate, sodium potassium tartrate, peptone and lactose were 192.5, 210, 225 and 325 mg/L, respectively. The stoichiometry ratio resulting from different OM differed largely and R1 could be as an indicator for OM inhibition. When COD concentration was 240 mg/L, the loss of SAA resulting from lactose, peptone, sodium potassium tartrate and sodium succinate were 28, 36, 50 and 55%, respectively. Sodium succinate had the highest inhibitory effect on SAA. When ANAMMOX process was used to treat wastewater containing OM, the modified Logistic model could be employed to predict the NREmax.
KeywordsNitrogen removal Organic matter ANAMMOX Denitrification Kinetic model
The work was financially supported by Shandong Provincial Natural Science Foundation (BS2015HZ007), Shandong Province Higher Educational Science and Technology Program (J15LC61) and Key Lab of Marine Bioactive Substances and Modern Analytical Technique (MBSMAT-2015-01).
- APHA (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DCGoogle Scholar
- Guven D, Dapena A, Kartal B, Schmid MC, Maas B, van de Pas-Schoonen K, Sozen S, Mendez R, Op den Camp HJ, Jetten MS, Strous M, Schmidt I (2005) Propionate oxidation by and methanol inhibition of anaerobic ammonium-oxidizing bacteria. Appl Environ Microbiol 71(2):1066–1071CrossRefPubMedPubMedCentralGoogle Scholar
- Kartal B, Rattray J, van Niftrik LA, van de Vossenberg J, Schmid MC, Webb RI, Schouten S, Fuerst JA, Damste JS, Jetten MS, Strous M (2007) Candidatus “ANAMMOXoglobus propionicus” a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria. Syst Appl Microbiol 30(1):39–49CrossRefPubMedGoogle Scholar
- van de Graaf AA, Mulder A, de Bruijn P, Jetten MS, Robertson LA, Kuenen JG (1995) Anaerobic oxidation of ammonium is a biologically mediated process. Appl Environ Microb 61(4):1246–1251Google Scholar
- Vlaeminck SE, Terada A, Smets BF, De Clippeleir H, Schaubroeck T, Bolca S, Demeestere L, Mast J, Boon N, Carballa M, Verstraete W (2010) Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and ANAMMOX. Appl Environ Microb 76(3):900–909CrossRefGoogle Scholar