Biodegradation

, Volume 27, Issue 2–3, pp 165–178 | Cite as

Effects of solid-phase denitrification on the nitrate removal and bacterial community structure in recirculating aquaculture system

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Abstract

A solid-phase denitrification (SPD) reactor packed with poly (3-hydroxybutyrate-co-3-hydroxyvalerate) as a carbon source was incorporated into a recirculating aquaculture system (RAS) to remove accumulated nitrate. Bacterial community structures in different parts of the RAS, including biofilter unit, SPD reactor, and culture water, were analyzed using Illumina MiSeq sequencing technology. The data showed that nitrate levels decreased remarkably in the RAS connected with SPD reactor (RAS-DR). In contrast, nitrate levels increased continuously in the conventional RAS without SPD reactor (RAS-CK). Biofilter unit and culture water in RAS-DR developed lower species richness and higher bacterial community diversity than that in RAS-CK. The bacterial community structure of RAS was significantly affected by the SPD process and the changes included an increase in the proportion of Proteobacteria and Firmicutes and a decrease in Nitrospira abundance in RAS-DR. Firmicutes was the most abundant phylum (56.9 %) and mainly consisted of Clostridium sensu stricto (48.3 %) in SPD reactor.

Keywords

Nitrate Solid-phase denitrification Microbial community Recirculating aquaculture system Illumina MiSeq sequencing 
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Notes

Acknowledgments

The authors are grateful for the financial support offered by the National Natural Science Foundation of China (Grant No. 21077014), Beijing Natural Science Foundation (Grant No. 8152016), and the Special Program for Creative Ability and Youth Science Foundation of Beijing Academy of Agriculture and Forestry Sciences (Grant No. KJCX 20140420, QNJJ 201417).

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Beijing Engineering Research Center of Environmental Material for Water PurificationBeijing University of Chemical TechnologyBeijingPeople’s Republic of China
  2. 2.Beijing Agro-Biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and BiotechnologyBeijing Academy of Agriculture and Forestry SciencesBeijingPeople’s Republic of China
  3. 3.School of Chemical EngineeringNortheast Dianli UniversityJilinPeople’s Republic of China
  4. 4.Heihe Hydrology Bureau of Heilongjiang ProvinceHeihePeople’s Republic of China

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