Biodegradation

, Volume 25, Issue 3, pp 447–457 | Cite as

Biodegradation of microcystin-LR and-RR by a novel microcystin-degrading bacterium isolated from Lake Taihu

  • Fei Yang
  • Yuanlong Zhou
  • Rongli Sun
  • Haiyan Wei
  • Yunhui Li
  • Lihong Yin
  • Yuepu Pu
Original Article

Abstract

Microcystin-LR (MC-LR) and microcystin-RR (MC-RR) are the two most common microcystins (MCs) present in fresh water posing a direct threat to public health because of their hepatotoxicity. A novel MC-degrading bacterium designated MC-LTH1 capable of degrading MC-LR and -RR was isolated, and the degradation rates and mechanisms of MC-LR and -RR for this bacterium were investigated. The bacterium was identified as Bordetella sp. and shown to possess a homologous mlrA gene responsible for degrading MCs. To the best of our knowledge, this is the first report of mlrA gene detection in Bordetella species. MC-LR and -RR were completely degraded separately at rates of 0.31 mg/(L h) and 0.17 mg/(L h). However, the degradation rates of MC-LR and -RR decreased surprisingly to 0.27 mg/(L h) and 0.12 mg/(L h), respectively, when both of them were simultaneously present. Degradation products were identified by high performance liquid chromatography coupled with time-of-flight mass spectrometry. Adda (m/z 332.2215, C20H29NO3) commonly known as a final product of MC degradation by isolated bacteria was detected as an intermediate in this study. Linearized MC-LR (m/z 1013.5638, C49H76N10O13), linearized MC-RR (m/z 1056.4970, C49H77N13O13), and tetrapeptide (m/z 615.3394, C32H46N4O8) were also detected as intermediates. These results indicate that the bacterial strain MC-LTH1 is quite efficient for the detoxification of MC-LR and MC-RR, and possesses significant bioremediation potential.

Keywords

Biodegradation Microcystin Adda Bordetella mlrA 

Notes

Acknowledgments

The authors are grateful to Michael Cunningham from the University of Maryland for help with language polishing, and Guangcan Zhu, Ran Yu, and Ying Zhan for help on HPLC–TOF-MS data analysis. This research was supported by the National Natural Science Foundation of China (30972440), the National Science and Technology Major Project (2012ZX07101-005), and the Jiangsu Province postgraduate Innovation Project (CX10B–087Z).

Supplementary material

10532_2013_9673_MOESM1_ESM.pdf (501 kb)
Supplementary material 1 (PDF 500 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Fei Yang
    • 1
  • Yuanlong Zhou
    • 1
  • Rongli Sun
    • 1
  • Haiyan Wei
    • 1
  • Yunhui Li
    • 1
  • Lihong Yin
    • 1
  • Yuepu Pu
    • 1
  1. 1.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public HealthSoutheast UniversityNanjingChina

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