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Biodegradation

, Volume 25, Issue 2, pp 301–312 | Cite as

Substrate interactions in dehalogenation of 1,2-dichloroethane, 1,2-dichloropropane, and 1,1,2-trichloroethane mixtures by Dehalogenimonas spp.

  • Jacob L. Dillehay
  • Kimberly S. Bowman
  • Jun Yan
  • Fred A. Rainey
  • William M. MoeEmail author
Original Article

Abstract

When chlorinated alkanes are present as soil or groundwater pollutants, they often occur in mixtures. This study evaluated substrate interactions during the anaerobic reductive dehalogenation of chlorinated alkanes by the type strains of two Dehalogenimonas species, D. lykanthroporepellens and D. alkenigignens. Four contaminant mixtures comprised of combinations of the chlorinated solvents 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), and 1,1,2-trichloroethane (1,1,2-TCA) were assessed for each species. Chlorinated solvent depletion and daughter product formation determined as a function of time following inoculation into anaerobic media revealed preferential dechlorination of 1,1,2-TCA over both 1,2-DCA and 1,2-DCP for both species. 1,2-DCA in particular was not dechlorinated until 1,1,2-TCA reached low concentrations. In contrast, both species concurrently dechlorinated 1,2-DCA and 1,2-DCP over a comparably large concentration range. This is the first report of substrate interactions during chlorinated alkane dehalogenation by pure cultures, and the results provide insights into the chlorinated alkane transformation processes that may be expected for contaminant mixtures in environments where Dehalogenimonas spp. are present.

Keywords

Dechlorination Dehalorespiration Inhibition Mixtures Dehalogenimonas 

Notes

Acknowledgments

This research was funded by NPC Services, Inc. and the Governor’s Biotechnology Initiative of the Louisiana Board of Regents Grant BOR#15 Enhancement of the LSU Hazardous Substance Research Center Environmental Biotechnology Initiative.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Jacob L. Dillehay
    • 1
  • Kimberly S. Bowman
    • 1
    • 2
  • Jun Yan
    • 1
    • 4
  • Fred A. Rainey
    • 2
    • 3
  • William M. Moe
    • 1
    Email author
  1. 1.Department of Civil and Environmental EngineeringLouisiana State UniversityBaton RougeUSA
  2. 2.Department of Biological SciencesLouisiana State UniversityBaton RougeUSA
  3. 3.Department of Biological SciencesUniversity of Alaska AnchorageAnchorageUSA
  4. 4.Department of Microbiology and Department of Civil and Environmental EngineeringUniversity of TennesseeKnoxvilleUSA

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