Biodegradation

, Volume 25, Issue 1, pp 111–125

Naphthalene biodegradation in temperate and arctic marine microcosms

  • Andrea Bagi
  • Daniela M. Pampanin
  • Anders Lanzén
  • Torleiv Bilstad
  • Roald Kommedal
Original Paper

Abstract

Naphthalene, the smallest polycyclic aromatic hydrocarbon (PAH), is found in abundance in crude oil, its major source in marine environments. PAH removal occurs via biodegradation, a key process determining their fate in the sea. Adequate estimation of PAH biodegradation rates is essential for environmental risk assessment and response planning using numerical models such as the oil spill contingency and response (OSCAR) model. Using naphthalene as a model compound, biodegradation rate, temperature response and bacterial community composition of seawaters from two climatically different areas (North Sea and Arctic Ocean) were studied and compared. Naphthalene degradation was followed by measuring oxygen consumption in closed bottles using the OxiTop® system. Microbial communities of untreated and naphthalene exposed samples were analysed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR–DGGE) and pyrosequencing. Three times higher naphthalene degradation rate coefficients were observed in arctic seawater samples compared to temperate, at all incubation temperatures. Rate coefficients at in situ temperatures were however, similar (0.048 day−1 for temperate and 0.068 day−1 for arctic). Naphthalene biodegradation rates decreased with similar Q10 ratios (3.3 and 3.5) in both seawaters. Using the temperature compensation method implemented in the OSCAR model, Q10 = 2, biodegradation in arctic seawater was underestimated when calculated from the measured temperate k1 value, showing that temperature difference alone could not predict biodegradation rates adequately. Temperate and arctic untreated seawater communities were different as revealed by pyrosequencing. Geographic origin of seawater affected the community composition of exposed samples.

Keywords

Hydrocarbons Naphthalene Biodegradation Q10 Pyrosequencing DGGE Seawater 

Abbreviations

PAH

Polycyclic aromatic hydrocarbon

HC

Hydrocarbon

OSCAR

Oil spill contingency and response

GC

Gas chromatography

PCR

Polymerase chain reaction

DGGE

Denaturing gradient gel electrophoresis

OTU

Operational taxonomic unit

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Andrea Bagi
    • 1
  • Daniela M. Pampanin
    • 2
  • Anders Lanzén
    • 3
  • Torleiv Bilstad
    • 1
  • Roald Kommedal
    • 1
  1. 1.Department of Mathematics and Natural Sciences, Faculty of Science and TechnologyUniversity of StavangerStavangerNorway
  2. 2.International Research Institute of StavangerStavangerNorway
  3. 3.Computational Biology Unit, Uni Computing, Uni Research and Department of BiologyUniversity of BergenBergenNorway

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