Biodegradation

, Volume 24, Issue 5, pp 615–630 | Cite as

Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp. strain I11

  • B. Almeida
  • H. Kjeldal
  • I. Lolas
  • A. D. Knudsen
  • G. Carvalho
  • K. L. Nielsen
  • M. T. Barreto Crespo
  • A. Stensballe
  • J. L. Nielsen
Original Paper
First Online:
Received:
Accepted:

Abstract

Ibuprofen is the third most consumed pharmaceutical drug in the world. Several isolates have been shown to degrade ibuprofen, but very little is known about the biochemistry of this process. This study investigates the degradation of ibuprofen by Patulibacter sp. strain I11 by quantitative proteomics using a metabolic labelling strategy. The whole-genome of Patulibacter sp. strain I11 was sequenced to provide a species-specific protein platform for optimal protein identification. The bacterial proteomes of actively ibuprofen-degrading cells and cells grown in the absence of ibuprofen was identified and quantified by gel based shotgun-proteomics. In total 251 unique proteins were quantitated using this approach. Biological process and pathway analysis indicated a number of proteins that were up-regulated in response to active degradation of ibuprofen, some of them are known to be involved in the degradation of aromatic compounds. Data analysis revealed that several of these proteins are likely involved in ibuprofen degradation by Patulibacter sp. strain I11.

Keywords

Aromatic compounds Ibuprofen Degradation Patulibacter sp.  Quantitative proteomics 
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Notes

Acknowledgments

The authors acknowledge the support of The Danish Research Council for Technology and Production to JLN. BA, GC and MTBC were supported by Fundação para a Ciência e Tecnologia through the grant Pest-OE/EQB/LA0004/2011, project grant PTDC/EBB-EBI/098862/2008, and fellowship grants SFRH/BD/47748/2008 (BA) and SFRH/BPD/30800/2006 (GC). IBET Analytical Services and Dr. Dulce Brito are thankfully acknowledged for their support with UPLC analysis of ibuprofen degradation.

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • B. Almeida
    • 1
    • 2
  • H. Kjeldal
    • 4
  • I. Lolas
    • 4
  • A. D. Knudsen
    • 4
  • G. Carvalho
    • 1
    • 3
  • K. L. Nielsen
    • 4
  • M. T. Barreto Crespo
    • 1
    • 2
  • A. Stensballe
    • 4
  • J. L. Nielsen
    • 4
  1. 1.IBETOeirasPortugal
  2. 2.Instituto de Tecnologia Química e Biológica, Universidade Nova de LisboaOeirasPortugal
  3. 3.REQUIMTE/CQFB, Department of ChemistryFCT, Universidade Nova de LisboaCaparicaPortugal
  4. 4.Department of Biotechnology, Chemistry and Environmental EngineeringAalborg UniversityAalborgDenmark

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