Biodegradation

, Volume 23, Issue 4, pp 487–496 | Cite as

Bio-reduction of Cr(VI) by exopolysaccharides (EPS) from indigenous bacterial species of Sukinda chromite mine, India

  • R. Harish
  • Jastin Samuel
  • R. Mishra
  • N. Chandrasekaran
  • A. Mukherjee
Original Paper

Abstract

Chrome mining activity has contributed intensively towards pollution of hexavalent chromium around Sukinda Valley, Orissa, India. In an attempt to study the specific contribution of exopolysaccharides (EPS) extracted from indigenous isolates towards Cr(VI) reduction, three chromium (VI) tolerant strains were isolated from the effluent mining sludge. Based on the tolerance towards Cr(VI) and EPS production capacity, one of them was selected for further work. The taxonomic identity of the selected strain was confirmed to be Enterobacter cloacae (showing 98% similarity in BLAST search to E. cloacae) through 16S rRNA analysis. The EPS production was observed to increase with increasing Cr(VI) concentration in the growth medium, highest being 0.078 at 100 mg/l Cr(VI). The extracted EPS from Enterobacter cloacae SUKCr1D was able to reduce 31.7% of Cr(VI) at 10 mg/l concentration, which was relevant to the prevailing natural concentrations at Sukinda mine effluent sludge. The FT-IR spectral studies confirmed the surface chemical interactions of hexavalent chromium with EPS.

Keywords

Enterobactercloacae SUKCr1D Bioremediation Sukinda Valley Exopolysaccharides (EPS) 

Notes

Acknowledgments

Authors thank the Department of Science and Technology (DST), Govt. of India for funding this project and also thank the management of VIT University for supporting research. The authors are deeply indebted to three anonymous reviewers and the managing editor of the journal for their valuable suggestions to substantially improve the quality of the manuscript.

Supplementary material

10532_2011_9527_MOESM1_ESM.pdf (47 kb)
Supplementary material 1 (PDF 48 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • R. Harish
    • 1
  • Jastin Samuel
    • 1
  • R. Mishra
    • 1
  • N. Chandrasekaran
    • 1
  • A. Mukherjee
    • 1
  1. 1.Centre for Nanobiotechnology, School of Biosciences and TechnologyVIT UniversityVelloreIndia

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