Biodegradation

, Volume 23, Issue 3, pp 387–397 | Cite as

Isolation of the opdE gene that encodes for a new hydrolase of Enterobacter sp. capable of degrading organophosphorus pesticides

  • Concepción Chino-Flores
  • Edgar Dantán-González
  • Alejandra Vázquez-Ramos
  • Raunel Tinoco-Valencia
  • Rafael Díaz-Méndez
  • Enrique Sánchez-Salinas
  • Ma. Luisa Castrejón-Godínez
  • Fernando Ramos-Quintana
  • Ma. Laura Ortiz-Hernández
Original Paper

Abstract

Microbial enzymes that can hydrolyze organophosphorus compounds have been isolated, identified and characterized from different microbial species in order to use them in biodegradation of organophosphorus compounds. We isolated a bacterial strain Cons002 from an agricultural soil bacterial consortium, which can hydrolyze methyl-parathion (MP) and other organophosphate pesticides. HPLC analysis showed that strain Cons002 is capable of degrading pesticides MP, parathion and phorate. Pulsed-field gel electrophoresis and 16S rRNA amplification were performed for strain characterization and identification, respectively, showing that the strain Cons002 is related to the genus Enterobacter sp. which has a single chromosome of 4.6 Mb and has no plasmids. Genomic library was constructed from DNA of Enterobacter sp. Cons002. A gene called opdE (Organophosphate Degradation from E nterobacter) consists of 753 bp and encodes a protein of 25 kDa, which was isolated using activity methods. This gene opdE had no similarity to any genes reported to degrade organophosphates. When kanamycin-resistance cassette was placed in the gene opdE, hydrolase activity was suppressed and Enterobacter sp. Cons002 had no growth with MP as a nutrients source.

Keywords

Methyl-parathion Organophosphorus pesticides Enterobacter sp. Cons002 opdE Gene Degradation 

Notes

Acknowledgments

We thank Blanca I. García-Gomez for generous assistance with sequencing. The research work of our groups was supported in part by CONACyT 93760 and grant CONSOLIDACIÓN-18-08.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Concepción Chino-Flores
    • 1
  • Edgar Dantán-González
    • 1
  • Alejandra Vázquez-Ramos
    • 2
  • Raunel Tinoco-Valencia
    • 2
  • Rafael Díaz-Méndez
    • 3
  • Enrique Sánchez-Salinas
    • 1
  • Ma. Luisa Castrejón-Godínez
    • 1
  • Fernando Ramos-Quintana
    • 4
  • Ma. Laura Ortiz-Hernández
    • 1
  1. 1.Laboratorio de Investigaciones Ambientales, Centro de Investigación en BiotecnologíaUniversidad Autónoma del Estado MorelosCuernavacaMexico
  2. 2.Instituto de BiotecnologíaUNAMCuernavacaMexico
  3. 3.Centro de Ciencias GenómicasUNAMCuernavacaMexico
  4. 4.Instituto Tecnológico de Estudios Superiores de MonterreyCuernavacaMexico

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