Biodegradation

, Volume 22, Issue 1, pp 95–102 | Cite as

Biodegradation of 2-Nitrotoluene by Micrococcus sp. strain SMN-1

  • Sikandar I. Mulla
  • Robertcyril S. Hoskeri
  • Yogesh S. Shouche
  • Harichandra Z. Ninnekar
Original Paper
First Online:
Received:
Accepted:

Abstract

A bacterial consortium capable of degrading nitroaromatic compounds was isolated from pesticide-contaminated soil samples by selective enrichment on 2-nitrotoluene as a sole source of carbon and energy. The three different bacterial isolates obtained from bacterial consortium were identified as Bacillus sp. (A and C), Bacillus flexus (B) and Micrococcus sp. (D) on the basis of their morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA gene sequences. The pathway for the degradation of 2-nitrotoluene by Micrococcus sp. strain SMN-1 was elucidated by the isolation and identification of metabolites, growth and enzymatic studies. The organism degraded 2-nitrotoluene through 3-methylcatechol by a meta-cleavage pathway, with release of nitrite.

Keywords

Bacterial consortium Nitroaromatics Micrococcus sp. strain SMN-1 2-Nitrotoluene 
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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sikandar I. Mulla
    • 1
  • Robertcyril S. Hoskeri
    • 1
  • Yogesh S. Shouche
    • 2
  • Harichandra Z. Ninnekar
    • 1
  1. 1.Department of BiochemistryKarnatak UniversityDharwadIndia
  2. 2.National Centre for Cell SciencePune University CampusPuneIndia

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