, Volume 22, Issue 1, pp 63–69 | Cite as

Degradation of pentachlorophenol by Kocuria sp. CL2 isolated from secondary sludge of pulp and paper mill

  • Santosh Kr. Karn
  • S. K. Chakrabarti
  • M. Sudhakara Reddy
Original Paper


A pentachlorophenol (PCP) degrading bacterium was isolated and characterized from sludge of pulp and paper mill. This isolate used PCP as its sole source of carbon and energy and was capable of degrading this compound, as indicated by stoichiometric release of chloride and biomass formation. Based on morphology, biochemical tests, and 16S rRNA gene sequence analysis this strain was identified as Kocuria sp. CL2. High Performance Liquid Chromatography (HPLC) analysis revealed that this strain was able to degrade PCP up to a concentration of 600 mg/l. This is first time we are reporting the degradation of PCP by the Kocuria species. This isolate was also able to remove 58.64% of PCP from the sludge within two weeks. This study showed that the removal efficiency of PCP by CL2 was found to be very effective and can be used in degradation of PCP containing pulp paper mill waste in the environment.


Kocuria sp. CL2 Sludge Biodegradation Characterization Chloride ion Pentachlorophenol 



The authors are thankful to TIFAC-CORE in Agro and Industrial Biotechnology, Thapar University for providing facilities.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Santosh Kr. Karn
    • 1
  • S. K. Chakrabarti
    • 2
  • M. Sudhakara Reddy
    • 1
  1. 1.Department of BiotechnologyThapar UniversityPatialaIndia
  2. 2.Thapar Centre for Industrial Research and DevelopmentYamunanagarIndia

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