, Volume 21, Issue 6, pp 979–987 | Cite as

Efficient biotransformation of herbicide diuron by bacterial strain Micrococcus sp. PS-1

  • Priyanka Sharma
  • Adity Chopra
  • Swaranjit Singh Cameotra
  • C. Raman Suri
Original Paper


A Gram-positive, Micrococcus sp. strain PS-1 capable of utilizing phenylurea herbicide diuron as a sole carbon source at a high concentration (up to 250 ppm) was isolated from diuron storage site by selective enrichment study. The taxonomic characterization with 16S rRNA gene sequencing (1,477 bp) identified PS-1 as a member of Micrococcus sp. It was studied for the degradation of diuron and a range of its analogues (monuron, linuron, monolinuron, chlortoluron and fenuron). The shake flasks experiments demonstrated fast degradation of diuron (up to 96% at 250 ppm within 30 h incubation) with the addition of small quantity (0.01%) of non-ionic detergent. The relative degradation profile by the isolate was in the order of fenuron > monuron > diuron > linuron > monolinuron > chlortoluron. Further, the biochemical characterization of catabolic pathway by spectroscopic and chromatographic techniques demonstrated that the degradation proceeded via formation of dealkylated metabolites to form 3,4-dichloroaniline (3,4-DCA). It was the major metabolite formed, associated with profound increase in degradation kinetics in presence of appropriate additive.


Micrococcus sp. Degradation Phenylurea herbicides Diuron 3,4-DCA 

Supplementary material

10532_2010_9357_MOESM1_ESM.doc (153 kb)
Supplementary materials (Media preparation and Biochemical analysis of the strain PS-1: ST; detailed analysis of screening of pesticide degrading strain: T1 and: S1 are attached separately. (DOC 153 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Priyanka Sharma
    • 1
  • Adity Chopra
    • 1
  • Swaranjit Singh Cameotra
    • 1
  • C. Raman Suri
    • 1
  1. 1.Institute of Microbial Technology (CSIR)ChandigarhIndia

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