Biodegradation

, Volume 19, Issue 6, pp 831–839 | Cite as

Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates

  • Jijian Yang
  • Chao Yang
  • Hong Jiang
  • Chuanling Qiao
Original Paper
First Online:
Received:
Accepted:

Abstract

The coding region of mpd gene corresponding to mature methyl parathion hydrolase (MPH) was heterologously overexpressed in Escherichia coli BL21 (DE3) by using pET expression system. The lactose-induced expression yield of MPH is increased 2-fold compared with IPTG as inducer. Furthermore, it was found that specific activity of MPH increased 48% by reducing the induction temperature to 22°C. The addition of 25 mM lactose at 22°C, the MPH activity of fermentation broth had a specific activity of 1.4 × 104 U/mg protein. Plasmid was no significant decrease in the modified medium. The optimal pH and temperature of MPH were 8.0 and 30°C, respectively. Over a period of 5 months, the dried cells showed no significant decrease in the activity of the detoxifying enzymes. The crude enzymes in 50 mM citrate-phosphate buffer (pH 8.0) were able to degrade about 98% of the organophosphate pesticides sprayed on cabbage. The detoxification efficiency was superior to that of the treatments of water, detergent, and a commercially available enzyme product. Additionally, the products of pesticide hydrolysis generated by treatment with the enzyme extract were determined to be virtually nontoxic.

Keywords

Decontamination Environmental health Fermentation Lactose Methyl parathion hydrolase 
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Notes

Acknowledgements

This work was supported by grants from the 863 Hi-Tech Research and Development Program of the People’s Republic of China (No. 2007AA06Z335) and the Innovation Program of the Chinese Academy of Sciences (No. KSCX2-YW-G-008). We appreciate valuable comments and suggestions of reviewers.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Jijian Yang
    • 1
    • 2
  • Chao Yang
    • 1
    • 2
  • Hong Jiang
    • 1
  • Chuanling Qiao
    • 1
  1. 1.State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of ZoologyChinese Academy of SciencesBeijingP.R. China
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingP.R. China

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