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Biodegradation

, Volume 19, Issue 5, pp 683–693 | Cite as

17α-ethinylestradiol cometabolism by bacteria degrading estrone, 17β-estradiol and estriol

  • Bram Pauwels
  • Klaas Wille
  • Herlinde Noppe
  • Hubert De Brabander
  • Tom Van de Wiele
  • Willy Verstraete
  • Nico Boon
Original Paper

Abstract

17α-ethinylestradiol (EE2), the active compound of the contraceptive pill, is a recalcitrant estrogen, which is encountered at ng/l levels in wastewater treatment plant (WWTP) effluents and rivers and can cause feminization of aquatic organisms. The aim of this study was to isolate micro-organisms that could remove such low EE2 concentrations. In this study, six bacterial strains were isolated from compost that cometabolize EE2 when metabolizing estrone (E1), 17β-estradiol (E2) and estriol (E3). The strains belong to the α, β and γ-Proteobacteria. All six strains metabolize E2 over E1, at μg/l to ng/l concentrations. In 4 days, initial concentrations of 0.5 μg E2/l and 0.6 μg EE2/l were degraded to 1.8 ± 0.4 ng E2/l and 85 ± 16 ng EE2/l, respectively. No other metabolites besides E1, E2, E3 or EE2 were detected, suggesting that total degradation and cleavage of the aromatic ring occurred. This is the first study describing that bacteria able to metabolize E2, can subsequently cometabolize EE2 at low μg/l levels.

Keywords

Estrogen Ethinylestradiol Cometabolism Acinetobacter sp. Pseudomonas sp. 

Abbreviations

E1

Estrone

E2

Estradiol

E3

Estriol

EE2

Ethinylestradiol

HRT

Hydraulic residence time

SRT

Sludge retention time

WWTP

Wastewater treatment plant

Notes

Acknowledgements

The authors wish to thank IWT Vlaanderen to support this research with a Ph.D. funding (Grant Number IWT-33298). Petra Vandamme is greatly acknowledged for performing the molecular analyses. The authors wish to thank Ilse Forrez and Lynn Vanhaecke for their valuable comments.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Bram Pauwels
    • 1
  • Klaas Wille
    • 1
  • Herlinde Noppe
    • 2
  • Hubert De Brabander
    • 2
  • Tom Van de Wiele
    • 1
  • Willy Verstraete
    • 1
  • Nico Boon
    • 1
  1. 1.Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bio-engineering ScienceGhent UniversityGentBelgium
  2. 2.Laboratory of Chemical Analysis, Faculty of Veterinary MedicineGhent UniversityMerelbekeBelgium

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