Biodegradation

, Volume 19, Issue 1, pp 15–19 | Cite as

A novel moderately halophilic bacterium for decolorizing azo dye under high salt condition

  • Jianbo Guo
  • Jiti Zhou
  • Dong Wang
  • Cunping Tian
  • Ping Wang
  • M. Salah Uddin
Original Paper

Abstract

Halomonas sp strain GTW was newly isolated from coastal sediments contaminated by chemical wastewater and was identified to be a member of the genus Halomonas by 16S rDNA sequence analysis and physical and biochemical tests. The optimal decolorization conditions were as follows: temperature 30°C, pH 6.5.0–8.5, NaCl 10–20% (w/v) and the optimal carbon source was yeast exact. The results of experiments demonstrated that the bacteria could decolorize different azo dyes under high salt concentration conditions, and the decolorization rate of five tested azo dyes could be above 90% in 24 h. The exploitation of the salt-tolerant bacteria in the bio-treatment system would be a great improvement of conventional biological treatment systems and the bio-treatment concept.

Keywords

Halophilic bacteria Decolorization Halomonas Azo dyes 

Notes

Acknowledgements

This study was supported by the Cross-Century Talent Fund of National Education Committee of China.

References

  1. Bhatt M, Zhao J, Monteil-Rivera F, Hawari J (2005) Biodegradation of cyclic nitramines by tropical marine sediment bacteria. J Ind Microbiol Biotechnol. 32(6):261–267CrossRefGoogle Scholar
  2. Bromley-challenor KCA, Knapp JS, Zhang Z, Gray NCC, Hetheridge MJ, Evans MR (2000). Decolorization of an azo dye by unacclimated activated sludge under anaerobic conditions. Water Res 34:4410–4418CrossRefGoogle Scholar
  3. Brown MA, De Vito SC (1993). Predicting azo dye toxicity. Crit Rev Environ Sci Technol 23:249–324CrossRefGoogle Scholar
  4. Carliell CM, Barclay SJ, Naidoo N, Buckley CA, Mulholland DA, Senior E (1994) Anaerobic decolorisation of reactive dyes in conventional sewage treatment processes. J. Water SA 20:341–344Google Scholar
  5. De Baere LA, Devocht M, Assche PV, Verstraete W (1984). Influence of high NaCl and NH4Cl salt levels on methanogenic associations. Water Res 18:543–648CrossRefGoogle Scholar
  6. EPA.(1997) Profile of the textile industry. Environmental Protection Agency, Washington, USAGoogle Scholar
  7. Guo JB, Zhou JT, Wang D, Wang J, Yu H (2005) Decolorization of dye wastewater with high salt concentration by the Acclimatized salt-tolerant cultures. J Environ Sci 17(6):984–988Google Scholar
  8. Hinteregger C, Streichsbier F (1997) Halomonas sp., a moderately halophilic strain, for biotreatment of saline phenolic waste-water. Biotechnol Lett 19(11):1099–1102CrossRefGoogle Scholar
  9. Hu TL (1998) Degradation of azo dye RP2B by Pseudomonas luteola. Water Sci Technol 38:299–306CrossRefGoogle Scholar
  10. Ivanova EP, Bakunina YI, Sawabe T, Hayashi K, Alexeeva YV, Zhukova NV, Nicolau DV, Zvaygintseva TN, Mikhailov VV (2002) Two species of culturable bacteria associated with degradation of brown algae Fucus evanescens. Microb Ecol 43(2):242–249CrossRefGoogle Scholar
  11. Manu B, Chauhari S (2003) Decolorization of indigo and azo dyes in semicontinuous reactors with long hydraulic retention time. Process Biochem 38:1213–1221CrossRefGoogle Scholar
  12. Moosvi S, Keharia H, Madamwar D (2005) Decolourization of textile dye Reactive Violet 5 by a newly isolated bacterial consortium RVM 11 1. World J Microbiol Biotechnol 21:667–672CrossRefGoogle Scholar
  13. Mufiel B, Wafa A, Vincent U, Thierry H (1999) DNA extraction from activated sludges. Curr Microbiol. 38:315–319CrossRefGoogle Scholar
  14. Pearcea CI, Lloydb JR, Guthriea JT (2003) The removal of colour from textile wastewater using whole bacterial cells: a review. Dyes Pigments 58:179–196CrossRefGoogle Scholar
  15. Seshadri S, Bishop PL, Agha AM (1994) Anaerobic/aerobic treatment of selected azo dyes in wastewater. Waste Manag 14:127–137CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jianbo Guo
    • 1
    • 2
  • Jiti Zhou
    • 1
  • Dong Wang
    • 1
  • Cunping Tian
    • 1
  • Ping Wang
    • 1
  • M. Salah Uddin
    • 1
  1. 1.School of Environmental and Biological Science and TechnologyDalian University of TechnologyDalianP.R. China
  2. 2.School of Environmental Science and EngineeringHebei University of Science and TechnologyShijiazhuangP.R. China

Personalised recommendations