Biodegradation

, Volume 18, Issue 3, pp 359–369

Enhancement of bioconversion of high-molecular mass polycyclic aromatic hydrocarbons in contaminated non-sterile soil by litter-decomposing fungi

  • Kari T. Steffen
  • Sven Schubert
  • Marja Tuomela
  • Annele Hatakka
  • Martin Hofrichter
Original Paper

Abstract

With the focus on alternative microbes for soil-bioremediation, 18 species of litter-decomposing basidiomycetous fungi were screened for their ability to grow on different lignocellulosic substrates including straw, flax and pine bark as well as to produce ligninolytic enzymes, namely laccase and manganese peroxidase. Following characteristics have been chosen as criteria for the strain selection: (i) the ability to grow at least on one of the mentioned materials, (ii) production of either of the ligninolytic enzymes and (iii) the ability to invade non-sterile soil. As the result, eight species were selected for a bioremediation experiment with an artificially contaminated soil (total polycyclic aromatic hydrocarbon (PAH) concentration 250 mg/kg soil). Up to 70%, 86% and 84% of benzo(a)anthracene, benzo(a)pyrene, and dibenzo(a,h)anthracene, respectively, were removed in presence of fungi while the indigenous microorganisms converted merely up to 29%, 26% and 43% of these compounds in 30 days. Low molecular-mass PAHs studied were easily degraded by soil microbes and only anthracene degradation was enhanced by the fungi as well. The agaric basidiomycetes Stropharia rugosoannulata and Stropharia coronilla were the most efficient PAH degraders among the litter-decomposing species used.

Keywords

Agrocybe Biodegradation Litter-decomposing fungi Polycyclic aromatic hydrocarbons Soil bioremediation Stropharia 

Abbreviations

BaP

Benzo(a)pyrene

LiP

Lignin peroxidase

LPO

Lipid peroxidation

LDF

Litter-decomposing fungi

MnP

Manganese peroxidase

PAHs

Polycyclic aromatic hydrocarbons

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Kari T. Steffen
    • 1
  • Sven Schubert
    • 2
    • 3
  • Marja Tuomela
    • 1
  • Annele Hatakka
    • 1
  • Martin Hofrichter
    • 2
  1. 1.Department of Applied Chemistry and MicrobiologyUniversity of HelsinkiHelsinkiFinland
  2. 2.Chair of Environmental BiotechnologyInternational Graduate School ZittauZittauGermany
  3. 3.Chair for Process BiotechnologyUniversity of BayreuthBayreuthGermany

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