, Volume 18, Issue 1, pp 115–121 | Cite as

Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes

  • Zulfiqar Ali Raza
  • Asma Rehman
  • Muhammad Saleem Khan
  • Zafar M. KhalidEmail author


Biosurfactant production by Pseudomonas aeruginosa EBN-8 mutant was studied in shake flasks on separate wastes from canola, soybean and corn oil refineries. Of the substrates tested, canola oil refinery waste (COD=20 g l−1) supplemented with sodium nitrate (at COD/N=20) showed the best microbial growth (4.50 g l−1) and rhamnolipid production (8.50 g l−1), at 10 d of incubation with the specific growth rate of 0.316 h−1 and specific product yield of 0.597 g g−1 h. Its cell-free supernatant showed the critical micelle dilution (CMD) of 150 and surface tension (ST) of 28.5 mN m−1.


biosurfactant emulsion Pseudomonas aeruginosa vegetable oil wastes yield factors 


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The authors acknowledge the Higher Education Commission, Islamabad for funding for this research, Ch. Muhammad Akram, General Manager, Madni Ghee Mills, Faisalabad for providing the VORWs samples and Mr. Muhammad Afzal, Senior Scientist, NIBGE for his lab’s facilities.


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Zulfiqar Ali Raza
    • 1
    • 2
  • Asma Rehman
    • 2
  • Muhammad Saleem Khan
    • 1
  • Zafar M. Khalid
    • 2
    Email author
  1. 1.Polymer Chemistry Laboratory, National Centre of Excellence in Physical ChemistryUniversity of PeshawarPeshawarPakistan
  2. 2.Environmental Biotechnology DivisionNational Institute for Biotechnology & Genetic EngineeringFaisalabadPakistan

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