Biodegradation

, Volume 18, Issue 1, pp 71–81 | Cite as

Kinetic analysis of the inhibitory effect of trichloroethylene (TCE) on nitrification in cometabolic degradation

Article

Abstract

In this study, the inhibitory effect of TCE on nitrification process was investigated with an enriched nitrifier culture. TCE was found to be a competitive inhibitor of ammonia oxidation and the inhibition constant (KI) was determined as 666–802 μg/l. The TCE affinity for the AMO enzyme was significantly higher than ammonium. The effect of TCE on ammonium utilization was evaluated with linearized plots of Monod equation (e.g., Lineweaver–Burk, Hanes–Woolf and Eadie–Hofstee plots) and non-linear least square regression (NLSR). No significant differences were found among these data evaluation methods in terms of kinetic parameters obtained.

Keywords

cometabolism kinetics modelling nitrification trichloroethylene 

Abbreviations

I:

inhibitory (non-growth) substrate concentration, μg non-growth substrate/l

KI:

inhibition constant of inhibitory (non-growth) substrate, μg non-growth substrate/l

Kiu:

dissociation constant of enzyme-substrate-inhibitory substance (ESI) complex, μg non-growth substrate/l

\(K_{\rm S}^{\rm app}\):

apparent half-saturation constant for growth substrate, mg substrate/l

KS:

half-saturation constant for growth substrate, mg growth substrate/l

q:

specific substrate utilization rate, mg substrate/g VSS h

\(q_{{\rm NH}_{4}\hbox {-}{\rm N}}\):

specific ammonium utilization rate, mg NH4-N/g VSS h

qmax:

maximum specific substrate utilization rate, mg substrate/g VSS h

\(q_{{\rm max, NH}_{4}\hbox {-}{\rm N}}\):

maximum specific ammonium utilization rate, mg NH4-N/g VSS h

\(q_{{\rm max, NH}_{4}\hbox {-}{\rm N}}^{\rm app}\):

apparent maximum specific ammonium utilization rate, mg NH4-N/g VSS h

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of Environmental EngineeringUniversity of MarmaraIstanbulTurkey
  2. 2.Institute of Environmental SciencesUniversity of BogaziciIstanbulTurkey

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