, Volume 17, Issue 5, pp 487–494 | Cite as

Characterization of a fungal strain capable of degrading chlorpyrifos and its use in detoxification of the insecticide on vegetables

  • Yun Long Yu
  • Hua Fang
  • Xiao Wang
  • Xiao Mao Wu
  • Min Shan
  • Jing Quan Yu
Original paper


A fungal strain capable of utilizing chlorpyrifos as sole carbon and energy sources was isolated from soil by enrichment cultivation approach. The half-lives of degradation (DT50) for chlorpyrifos at concentrations of 1, 10, and 100 mg l−1 by the fungal strain DSP in mineral salt medium were measured to be 2.03, 2.93, and 3.49 days, respectively. Two cell-free extracts [E (1:10) and E (1:20)] from the fungal strain DSP in bran–glucose medium were prepared and used to enhance chlorpyrifos degradation on vegetables. Compared with the controls, the DT50 of chlorpyrifos were reduced by 70.3%, 65.6%, 80.6%, 80.6%, and 86.1%, and by 53.8%, 43.2%, 66.0%, 54.3%, and 67.7% on E (1:20) and E (1:10) treated pakchoi, water spinach, Malabar spinach, haricot beans, and pepper, respectively. The 7-day residual values (R 7) of chlorpyrifos on E (1:10) treated vegetables were all lower than the corresponding maximum residue levels of European Union (EU MRLs), except that the R 7 value on haricot beans was slightly higher than the corresponding EU MRLs. The results indicate that cell-free extracts could rapidly degrade chlorpyrifos residues on vegetables.


biodegradation cell-free extract chlorpyrifos microorganism residue vegetable 


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This work was supported by National Natural Science Foundation of China (Nos. 20377036, 30230250, and 30270880), the Major State Basic Research Development Programme of China (2002CB410806), Zhejiang Provinicial Natural Science Foundation (301050) and Hangzhou Committee of Science and Technology (2002112A08).


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Copyright information

© Springer 2006

Authors and Affiliations

  • Yun Long Yu
    • 1
  • Hua Fang
    • 1
  • Xiao Wang
    • 1
  • Xiao Mao Wu
    • 1
  • Min Shan
    • 1
  • Jing Quan Yu
    • 2
  1. 1.Department of Plant ProtectionCollege of Agriculture & Biotechnology, Zhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of HorticultureCollege of Agriculture & Biotechnology, Zhejiang UniversityHangzhouPeople’s Republic of China

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