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Biodegradation

, Volume 16, Issue 6, pp 581–589 | Cite as

Biodegradation of beta-cyfluthrin by Pseudomonas stutzeri strain S1

  • Nirmali Saikia
  • Subrata K. Das
  • Bharat K. C. Patel
  • Ram Niwas
  • Aqbal Singh
  • Madhuban Gopal
Article

Abstract

β-Cyfluthrin [α-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate] pesticide has been in agricultural use in the recent years for controlling Lepidopteran pests affecting solanaceous crops. The extensive use of synthetic pyrethroids like β-cyfluthrin has resulted in wide spread environmental contamination. The purpose of this study was to isolate bacteria from soil and to determine their ability to degrade β-cyfluthrin and identify the intermediates in culture broth using spectroscopy. An aerobic bacterium capable of degrading β-cyfluthrin was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain S1) had 100% identity to the sequence from Pseudomonas stutzeri. Finally products formed during degradation of β-cyfluthrin have been identified as α-cyano-4-fluoro-3-phenoxybenzyl-3(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate (M.W. 341); 4-fluoro-3-phenoxy-α-cyanobenzyl alcohol (M.W. 243) and 3(2,2-dichlorovinyl)-2,2-dimethyl cyclopropanecarboxylic acid (M.W. 208).

Keywords

biodegradation β-cyfluthrin pseudomonas stutzeri synthetic pyrethroid 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Nirmali Saikia
    • 1
  • Subrata K. Das
    • 2
  • Bharat K. C. Patel
    • 3
  • Ram Niwas
    • 1
  • Aqbal Singh
    • 4
  • Madhuban Gopal
    • 1
  1. 1.Division of Agricultural ChemicalsIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.Department of BiotechnologyInstitute of Life Sciences Bhubaneswar India
  3. 3.School of Biomolecular and Biomedical Sciences, Faculty of Science and TechnologyGriffith UniversityBrisbaneAustralia
  4. 4.National Research Centre on Plant BiotechnologyIndian Agricultural Research InstituteNew DelhiIndia

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