Biodegradation

, Volume 16, Issue 5, pp 393–402

Isolation and characterization of phenanthrene-degrading Sphingomonas paucimobilis strain ZX4

  • Ying Xia
  • Hang Min
  • Gang Rao
  • Zhen-mei Lv
  • Ji Liu
  • Yang-fang Ye
  • Xue-jun Duan
Article

DOI: 10.1007/s10532-004-2412-7

Cite this article as:
Xia, Y., Min, H., Rao, G. et al. Biodegradation (2005) 16: 393. doi:10.1007/s10532-004-2412-7

Abstract

Phenanthrene-degrading bacterium strain ZX4 was isolated from an oil-contaminated soil, and identified as Sphingomonaspaucimobilis based on 16S rDNA sequence, cellular fatty acid composition, mol% G + C and Biolog-GN tests. Besides phenanthrene, strain ZX4 could also utilize naphthalene, fluorene and other aromatic compounds. The growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2, 3-dioxygenase revealed catechol could be metabolized through meta-cleavage pathway. Three genes, including two of meta-cleavage operon genes and one of GST encoding gene were obtained. The order of genes arrangement was similar to S-type meta-pathway operons. The phylogenetic trees based on 16S rDNA sequence and meta-pathway gene both revealed that strain ZX4 is clustered with strains from genus Sphingomonas.

Keywords

16S rDNA meta-cleavage operon genes phenanthrene degradation phylogenetic analysis 

Copyright information

© Springer 2005

Authors and Affiliations

  • Ying Xia
    • 1
    • 2
  • Hang Min
    • 1
  • Gang Rao
    • 3
  • Zhen-mei Lv
    • 1
  • Ji Liu
    • 1
  • Yang-fang Ye
    • 1
  • Xue-jun Duan
    • 1
  1. 1.College of Life SciencesZhejiang UniversityHangzhouChina
  2. 2.Microbiology InstituteChinese Academy of SciencesBeijingChina
  3. 3.State Intellectual Property Office of P.R. ChinaBeijingChina

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