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Biodiversity and Conservation

, Volume 20, Issue 2, pp 345–358 | Cite as

Dormancy release and seed ageing in the endangered species Silene diclinis

  • Sara Mira
  • M. Elena González-Benito
  • Ana M. Ibars
  • Elena EstrellesEmail author
Original Paper

Abstract

The influence of seed testa color, temperature and seed water content on dormancy release and seed viability loss in the endangered, endemic species Silene diclinis (Lag.) M. Laínz was evaluated. Dormant heterogeneous seeds (black, red and grey colored) were exposed to three different temperatures (5, 20, and 35°C) and two relative humidities (33 and 60%) in order to assay their dormancy release. Longevity behavior was studied for the three colored seeds, storing samples at nine different combinations of temperature (5, 20 and 35°C) and relative humidities (33, 60 and 90%). According to our findings, seed heteromorphism was not related to neither break of dormancy nor seed storage behavior. Silene diclinis seeds present dormancy after collection, and need an after-ripening period to germinate. Temperature and relative humidity are positively correlated with dormancy release and seed ageing. Therefore, both factors must be carefully controlled during seed manipulation in the laboratory for long term seed conservation purposes. When seeds are stored immediately after collection (dormant), if the temperature of storage is above the base temperature for dormancy release found in this work (between 2.7 and 1.6°C), seeds may eventually overcome dormancy. On the other hand if seeds are stored after an after-ripening period, storage at low temperature does not induce secondary dormancy.

Keywords

Caryophyllaceae Dormancy release Endangered plants Ex situ conservation Seed ageing Seed coat color Seed heterogeneity Seed longevity Silene diclinis Storage conditions 

Abbreviations

DRR

Dormancy release rate

fw

Fresh weigh

G

Germination percentage

RH

Relative humidity

θ

Thermal time

t

Time

T

Temperature

Tb

Base temperature for dormancy release

wc

Water content

Notes

Acknowledgements

This work was supported by the project CGL2006-10536 (Ministerio de Educación y Ciencia, Spain). S.M. was supported by a FPU program (Ministerio de Educación y Ciencia, Spain). The authors thank Marcelino de la Cruz (UPM) for his help with R program.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sara Mira
    • 1
  • M. Elena González-Benito
    • 1
  • Ana M. Ibars
    • 2
  • Elena Estrelles
    • 2
    Email author
  1. 1.Departamento de Biología Vegetal, Escuela Universitaria de Ingeniería Técnica AgrícolaUniversidad Politécnica de MadridMadridSpain
  2. 2.ICBiBE-Jardí BotànicUniversitat de ValènciaValenciaSpain

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