Biodiversity & Conservation

, Volume 14, Issue 6, pp 1433–1444 | Cite as

The use of RAPD markers for detecting genetic diversity, relationship and molecular identification of Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in a tea germplasm repository

  • Liang Chen
  • Qi-kang Gao
  • Da-ming Chen
  • Chang-jie Xu
Article

Abstract.

The genetic diversity, relationship and molecular identification of 15 well known, widely planted traditional Chinese elite tea genetic resources [Camellia sinensis (L.) O. Kuntze] preserved in the China National Germplasm Hangzhou Tea Repository in the Tea Research Institute of the Chinese Academy of Agricultural Sciences located in Zhejiang province, China, were investigated using RAPD markers. A total of 1050 bands with an average of 52.5 bands per primer, 70 bands per genetic resource were generated by the 20 selected primers from the 15 tea genetic resources. In the total of 137 amplified products, 129 were polymorphic, corresponding to 94.2% genetic diversity. The relative frequency of polymorphic products was from 0.24 to 0.83, with an average of 0.47. In general, this average frequency was relatively high. The genetic distances among the genetic resources were from 0.16 to 0.62, with an average of 0.37. The 15 tea genetic resources were grouped into three groups by UPGMA cluster analysis based on RAPD data. By using the presence of 20 unique RAPD markers and the absence of 11 unique markers, all the 15 investigated tea genetic resources could be easily identified. RAPD markers provided a practical method not only to evaluate the genetic diversity and relationship, but also to identify tea genetic resources.

Keywords

Genetic diversity Genetic relationship Molecular identification RAPD Tea genetic resources (Camellia sinensis

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References

  1. 1.
    Belaj, A., Trujilo, I., Rosa, R., Rallo, L., Gimenez, M.J. 2001Polymorphism and discrimination capacity of randomly amplified polymorphic markers in an olive germplasm bankJournal of the American Society for Horticultural Science1266471Google Scholar
  2. 2.
    Borse, B.B., Rao, J.M., Nagalakshmi, S., Krishnamurthy, N. 2002Fingerprint of black teas from India: identification of regio-specific characteristicsFood Chemistry79419424Google Scholar
  3. 3.
    Chen, L., Chen, D.M., Gao, Q.K., Yang, Y.J., Yu, F.L. 1997Isolation and Appraisal of Genomic DNA from Tea Plant [Camellia sinensis (L) O Kuntze]Journal of Tea Science17177181Google Scholar
  4. 4.
    Chen, L., Gao, Q.K., Yang, Y.J., Yu, F.L., Chen, D.M. 1998Optimum amplification procedure and reaction system for RAPD analysis of tea plants [Camellia sinensis (L.) O. Kuntze]Journal of Tea Science181620Google Scholar
  5. 5.
    Chen, L., Yamaguchi, S. 2002Genetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysisJournal of Horticultural Science and Biotechnology77729732Google Scholar
  6. 6.
    Chen, L., Yu, F.L., Yang, Y.J., Chen, D.M., Xu, C.J., Gao, Q.K. 1999A study on genetic stability of excellent tea germplasms [Camellia sinensis (L.) O. Kuntze] using RAPD markersJournal of Tea Science191113Google Scholar
  7. 7.
    Conner, P.J., Wood, B.W. 2001Identification of pecan cultivars and their genetic relatedness as determined by randomly amplified polymorphic DNA analysisJournal of the American Society for Horticultural Science126474480Google Scholar
  8. 8.
    Dhanaraj, A.L., Rao, E.V.V.B., Swamy, K.R.M., Bhat, M.G., Parsad, D.T., Sondur, S.N. 2002Using RAPDs to assess the diversity in India cashew (Anacarditum occidentale L.) germplasmJournal of Horticultural Science and Biotechnology774147Google Scholar
  9. 9.
    Galderisi, U., Cipollaro, M., Bernardo, G.D., Masi, L.D., Galano, G., Cascino, A. 1999Identification of hazelnut (Corylus avellana) cultivars by RAPD analysisPlant Cell Reports18652655Google Scholar
  10. 10.
    Hackett, C.A., Wachira, F.N., Paul, S., Powell, W., Waugh, R. 2000Construction of a genetic linkage map for Camellia sinensis (tea)Heredity85346355PubMedGoogle Scholar
  11. 11.
    Hashimoto, M., Takasi, S. 1978Morphological studies on the origin of the tea plant V: a proposal of one place of origin by cluster analysisJapanese Journal of Tropical Agriculture2193101Google Scholar
  12. 12.
    Hu, J., Quiros, C.F. 1991Identification of broccoli and cauliflower cultivars with RAPD markersPlant Cell Reports10505511Google Scholar
  13. 13.
    Kaundun, S.S., Zhyvoloup, A., Park, Y.G. 2000Evaluation of the genetic polymorphism among elite tea (Camellia sinensis var sinensis) accessions using RAPD markersEuphytica115716Google Scholar
  14. 14.
    Khasa, P.D., Dancik, B.P. 1996Rapid identification of white-Engelmann spruce species by RAPD markersTheoretical and Applied Genetics924652Google Scholar
  15. 15.
    Landry, B.S., Li, R.Q., Cheung, W.Y., Granger, R.L. 1994Phylogeny analysis of 25 apple rootstocks using RAPD markers and tactical gene taggingTheoretical and Applied Genetics89847852Google Scholar
  16. 16.
    Lee, S.H., Choi, H.S., Kim, R.H., Lee, H.Y., Nou, I.S. 1995Identification of Korean wild tea plants and Japanese green tea cultivars using RAPD markersJournal of the Korean Tea Society1129148Google Scholar
  17. 17.
    Lu, C.Y., Liu, W.H., Li, M.J. 1992Relationship between the evolutionary relatives and the variation of esterase isozymes in tea plantJournal of Tea Science121520Google Scholar
  18. 18.
    Mirshra, R.K., Sen-Mandi, S. 2001

    Genome analysis and isozyme studies for developing molecular markers associated with drought tolerance in tea plant

    Proceedings of 2001 International Conference on O-Cha (Tea) Culture and Science (Session II), 5–8 October 2001ShizuokaJapan6669
    Google Scholar
  19. 19.
    Mondal, T.K., Chand, P.K. 2002Detection of genetic variation among micropropagated tea [Camellia sinensis (L.) O. Kuntze] by RAPD analysisIn Vitro Cellular and Development Biology-Plant38296299Google Scholar
  20. 20.
    Nei, M., Li, W.H. 1979Mathematical model for studying genetic variation in terms of restriction endonucleaseProceedings of the National Academy of Science USA7652695273Google Scholar
  21. 21.
    Oh, M.J. 1994Studies on genetic relationship among the Korean native tea trees and physico-chemical properties of its green teaDepartment of AgricultureKorean UniversityKorea2729PhD dissertationGoogle Scholar
  22. 22.
    Orozco-Castillo, C., Chalmers, K.J., Powell, W., Waugh, R. 1996RAPD and organelle specific PCR re-affirms taxonomic relationships within the genus CoffeaPlant Cell Reports15337341Google Scholar
  23. 23.
    Park, Y.G., Kaundun, S.S., Zhyvoloup, A. 2002Use of the bulked genomic DNA-based RAPD methodology to access the genetic diversity among abandoned Korean tea plantationsGenetic Resources and Crop Evolution49159165Google Scholar
  24. 24.
    Sedra, M.H., Lasherms, P., Trouslot, P., Combes, M., Hamon, S. 1998Identification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markersEuphytica1037582Google Scholar
  25. 25.
    Shu, J.L., Chen, L., Wang, H.S., Wang, P.S., Xu, M., Song, W.X. 1998Pollen morphology, ultrastructure and evolution of tea plants and other genus Camellia plantsJournal of Tea Science18717Google Scholar
  26. 26.
    Takeda, Y. 2000

    History and development in Japanese tea breeding

    Park, Y.G.Shin, D.I. eds. Tea CultureTea Food Industry and Tea Breeding in KoreaChina and JapanThe Korea Tea SocietyKorea139158
    Google Scholar
  27. 27.
    Takeo, T., You, X.Q., Wang, H.F., Kinukasa, H., Li, M.J., Cheng, Q.K., Wang, H.S. 1992One speculation on the origin and dispersion of tea plant in China – One speculation based on the chemotaxonomy by using the contest-ration of terpene – alcohols found in the aroma compositionJournal of Tea Science128186Google Scholar
  28. 29.
    Tanaka, J., Yamaguchi, S. 1996Use of RAPD markers for the identification of parentage of tea cultivarsBulletin of the National Institute of Vegetables, Ornamental Plants & TeaJapan93136Google Scholar
  29. 28.
    Tanaka, J., Yamaguchi, N., Nakamura, Y. 2001Pollen parent of tea cultivar Sayamakaori with insect and cold resistance may not existBreeding Research34348Google Scholar
  30. 30.
    Tang, Q.Y., Feng, M.G. 1997Practical Statistics and DPS Data Processing SystemChina Agricultural PressBeijingp. 407Google Scholar
  31. 31.
    Wachira, F.N., Powell, W., Waugh, R. 1997An assessment of genetic diversity among Camellia sinensis L. (cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle-specific STSHeredity78603611Google Scholar
  32. 32.
    Wachira, F.N., Waugh, R., Hackett, C.A., Powell, W. 1995Detection of genetic diversity in tea (Camellia sinensis) using RAPD markersGenome38201210PubMedGoogle Scholar
  33. 34.
    Wang, Y., Lin, F.Y., Shi, L.M. 1994Investigation on the mtDNA polymorphism from the natural population of Drosophila sp, (II) Origin and differentiation of Drosophila spActa Genetica Sinica21263274Google Scholar
  34. 33.
    Wang, X.Q., Zou, Y.P., Zhang, D.M., Hong, D.Y. 1996RAPD analysis on genetic polymorphism of silvery firScience in China Series C26436444Google Scholar
  35. 35.
    Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V. 1990DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Research1865316535PubMedGoogle Scholar
  36. 36.
    Yu, F.L. 1986Discussion on the originating place and the originating center of tea plantJournal of Tea Science618Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Liang Chen
    • 1
  • Qi-kang Gao
    • 2
  • Da-ming Chen
    • 3
  • Chang-jie Xu
    • 3
  1. 1.Laboratory for Germplasm, Breeding and Molecular Biology, Tea Research InstituteChinese Academy of Agricultural Sciences; Key Laboratory of Tea Chemical Engineering, Ministry of AgricultureHangzhouChina
  2. 2.Biological Macromolecular Research Laboratory of Analyzing and Measurement CenterChina
  3. 3.Department of HorticultureZhejiang UniversityHangzhouChina

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