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Biological Invasions

, Volume 15, Issue 7, pp 1537–1559 | Cite as

Microsatellite population genetics of the emerald ash borer (Agrilus planipennis Fairmaire): comparisons between Asian and North American populations

  • Carson C. KeeverEmail author
  • Christal Nieman
  • Larissa Ramsay
  • Carol E. Ritland
  • Leah S. Bauer
  • D. Barry Lyons
  • Jenny S. Cory
Original Paper

Abstract

The emerald ash borer (EAB) (Agrilus planipennis Fairmaire) (Coleoptera; Buprestidae), is an invasive wood-boring beetle native to northeast Asia. This species was first detected in Michigan USA in 2002, and is a significant threat to native and ornamental ash tree species (Fraxinus spp.) throughout North America. We characterized seven polymorphic microsatellite markers for EAB and used these to investigate EAB population structure in the early invasive populations within North America and in comparison with Asia. We found 2–9 alleles per microsatellite locus, no evidence of linkage disequilibrium, and no association with known coding sequences, suggesting that these markers are suitable for population genetic analysis. Microsatellite population genetic structure was examined in 48 EAB populations sampled between 2003 and 2008 from five regions, three in the introduced range, Michigan (US) and Ontario and Quebec (Canada) and two Asian regions, China and South Korea, where EAB is native. We found significant genetic variation geographically but not temporally in EAB populations. Bayesian clustering analyses of individual microsatellite genotypes showed strong clustering among multiple North American populations and populations in both China and South Korea. Finally, allelic richness and expected heterozygosity were higher in the native range of EAB, but there was no difference in observed heterozygosity, suggesting a significant loss of alleles upon introduction but no significant change in the distribution of alleles within and among individuals.

Keywords

Microsatellite Invasive species Genetic diversity Genetic bottleneck 

Notes

Acknowledgments

Microsatellite development and analysis was funded by the Canadian Food Inspection Agency. Additional support was provided by SFU President’s Research start-up grant. We gratefully acknowledge the assistance of G. C. Jones (CFS) in sampling EAB populations in Ontario and Michigan; H. P. Liu (Michigan State University), R. T. Gao and T. H. Zhao (Chinese Academy of Forestry), and D. L. Miller and T. Petrice (USDA FS NRS) for assisting with sampling, rearing, and maintaining EAB samples from China; D. Williams (USDA APHIS Otis) for providing EAB samples from S. Korea. We would also like to thank Michelle Franklin for her comments on the manuscript.

Supplementary material

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Supplementary material 1 (DOCX 62 kb)
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Supplementary material 2 (DOCX 41 kb)
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Supplementary material 3 (DOCX 147 kb)
10530_2012_389_MOESM4_ESM.pdf (612 kb)
Supplementary material 4 (PDF 611 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Carson C. Keever
    • 1
    Email author
  • Christal Nieman
    • 2
    • 3
  • Larissa Ramsay
    • 2
    • 4
  • Carol E. Ritland
    • 3
  • Leah S. Bauer
    • 5
  • D. Barry Lyons
    • 4
  • Jenny S. Cory
    • 1
    • 2
  1. 1.Department of Biological SciencesSimon Fraser UniversityBurnabyCanada
  2. 2.Department of BiologyAlgoma UniversitySault Ste. MarieCanada
  3. 3.Department of Forest Sciences, Genetic Data CenterUniversity of British ColumbiaVancouverCanada
  4. 4.Natural Resources Canada, Canadian Forest ServiceSault Ste. MarieCanada
  5. 5.USDA Forest Service Northern Research StationE. LansingUSA

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