Biological Invasions

, Volume 10, Issue 7, pp 1135–1145 | Cite as

Quantitative genetic approach for assessing invasiveness: geographic and genetic variation in life-history traits

  • Sanna Boman
  • Alessandro Grapputo
  • Leena Lindström
  • Anne Lyytinen
  • Johanna Mappes
Original Paper


Predicting the spread of invasive species is a challenge for modern ecology. Although many invasive species undergo genetic bottlenecks during introduction to new areas resulting in a loss of genetic diversity, successful invaders manage to flourish in novel environments either because of pre-adaptations or because important traits contain adaptive variation enabling rapid adaptation to changing conditions. To predict and understand invasion success, it is crucial to analyse these features. We assessed the potential of a well-known invader, the Colorado potato beetle (Leptinotarsa decemlineata), to expand north of its current range in Europe. A short growing season and harsh overwintering conditions are apparent limiting factors for this species’ range. By rearing full-sib families from four geographically distinct populations (Russia, Estonia, Poland, Italy) at two fluctuating temperature regimes, we investigated (a) possible differences in survival, development time, and body size among populations and (b) the amount of adaptive variation within populations in these traits. All populations were able to complete their development in cooler conditions than in their current range. A significant genotype–environment interaction for development time and body size suggests the presence of adaptive genetic variation, indicating potential to adapt to cooler conditions. The northernmost population had the highest survival rates and fastest development times on both temperature regimes, suggesting pre-adaptation to cooler temperatures. Other populations had minor differences in development times. Interestingly, this species lacks the classical trade-off between body size and development time which could have contributed to its invasion potential. This study demonstrates the importance of considering both ecological and evolutionary aspects when assessing invasion risk.


Adaptation Additive genetic variation Geographical variation Invasive species 



We thank J. Haimi for technical assistance; S. Fasulati, K. Hiiesaar and M. Pawinska for the specimens; and M. Björklund and the journal club for comments on the manuscript. This experiment was done under licence (Dnro 28/420/2003) from the KTTK and financed by the Academy of Finland [project numbers 105926, 102292 (to LL) and 103201, 108335 (to AL)] and the Finnish Ministry of Agriculture and Forestry.


  1. Blanckenhorn WU (1994) Fitness consequences of alternative life histories in water striders, Aquarius remigis (Heteroptera: Gerridae). Oecologia 97:354–365Google Scholar
  2. Blanckenhorn WU, Hosken DJ (2003) Heritability of three condition surrogates in the yellow dung fly. Behav Ecol 14:612–618CrossRefGoogle Scholar
  3. Brakefield PM, Kesbeke F (1997) Genotype–environment interactions for insect growth in constant and fluctuating temperature regimes. Proc R Soc Lond B 264:717–723CrossRefGoogle Scholar
  4. Carroll SP, Dingle H (1996) The biology of post-invasion events. Biol Conserv 78:207–214CrossRefGoogle Scholar
  5. Casagrande RA (1985) The “Iowa” potato beetle, its discovery and spread to potatoes. Bull Entomol Soc Am 31:27–29Google Scholar
  6. Conover DO, Schultz ET (1995) Phenotypic similarity and the evolutionary significance of countergradient variation. Trends Ecol Evol 10:248–252CrossRefGoogle Scholar
  7. EPPO (2006) European and Mediterranean plant protection organization. Distribution maps of quarantine pests for Europe, Leptinotarsa decemlineata [WWW document]. URL http://www LPTNDE.pdf
  8. Falconer DS, Mackay TF (1996) Introduction to quantitative genetics, 4th edn. Longman, HarlowGoogle Scholar
  9. Fitzpatrick MC, Weltzin JF, Sanders NJ, Dunn RR (2006) The biogeography of prediction error: why does the introduced range of the fire and over-predict its native range? Glob Ecol Biogeogr 16:24–33CrossRefGoogle Scholar
  10. Forister ML, Ehmer AG, Futuyma DJ (2007) The genetic architecture of a niche: variation and covariation in host use traits in the Colorado potato beetle. J Evol Biol 20:985–996PubMedCrossRefGoogle Scholar
  11. Fox CW, Czesak ME, Savalla UM (1999) Environmentally based maternal effects on development time in the seed beetle Stator pruininus (Coleoptera: Bruchidae): consequences of larval density. Popul Ecol 28:217–223Google Scholar
  12. Garcia-Ramos G, Rodriguez D (2002) Evolutionary speed of species invasions. Evolution 56:661–668PubMedCrossRefGoogle Scholar
  13. Gaston KJ (2003) The structure and dynamics of geographic ranges. Oxford University Press, New YorkGoogle Scholar
  14. Genovesi P (2005) Eradication of invasive species in Europe: a review. Biol Invasions 7:127–133CrossRefGoogle Scholar
  15. Gihlcrist GW, Lee CE (2007) All stressed out and nowhere to go: does evolvability limit adaptation in invasive species? Genetica 129:127–132CrossRefGoogle Scholar
  16. Grapputo A, Boman S, Lindström L, Lyytinen A, Mappes J (2005) The voyage of an invasive species across continents: genetic diversity of North American and European Colorado potato beetle populations. Mol Ecol 14:4207–4219PubMedCrossRefGoogle Scholar
  17. Griffith TM, Watson MA (2006) Is evolution necessary for range expansion? Manipulating reproductive timing of a weedy annual transplanted beyond its range. Am Nat 167:153–164PubMedCrossRefGoogle Scholar
  18. Guisan A, Thuiller W (2005) Predicting species distribution: offering more than simple habitat models. Ecol Lett 8:993–1009CrossRefGoogle Scholar
  19. Hansen TF, Pélavon C, Armbruster WS, Carlson ML (2003) Evolvability and genetic constraint in Dalechampia blossoms: components of variance and measurements of evolvability. J Evol Biol 16:754–766PubMedCrossRefGoogle Scholar
  20. Hoffmann AA, Blows MW (1994) Species borders: ecological and evolutionary perspectives. Trends Ecol Evol 9:223–227CrossRefGoogle Scholar
  21. Hoffmann AA, Hallas RJ, Dean JA, Schiffer M (2003) Low potential for climatic stress Adaptation in a rainforest Drosophila species. Science 301:100–102PubMedCrossRefGoogle Scholar
  22. Holbrook GL, Schal C (2004) Maternal investment affects offspring phenotypic plasticity in a viviparous cockroach. Proc Natl Acad Sci USA 101:5595–5597PubMedCrossRefGoogle Scholar
  23. Houle D (1992) Comparing evolvability and variability of quantitative traits. Genetics 130:195–204PubMedGoogle Scholar
  24. Jeffree CE, Jeffree EP (1996) Redistribution of the potential geographical ranges of mistletoe and Colorado beetle in Europe in response to the temperature component of climate change. Funct Ecol 10:562–577CrossRefGoogle Scholar
  25. Johnson CG (1967) International dispersal of insects and insect-borne viruses. Neth J Plant Pathol 73:21–43CrossRefGoogle Scholar
  26. Kause A, Morin J-P (2001) Seasonality and genetic architecture of development time and body size of the birch feeding sawfly Priophorus pallies. Genet Res 78:31–40PubMedCrossRefGoogle Scholar
  27. Kawecki TJ, Ebert D (2004) Conceptual issues in local adaptation. Ecol Lett 7:1225–1241CrossRefGoogle Scholar
  28. Klingenberg CP, Spence JR (1997) On the role of body size for life-history evolution. Ecol Entomol 22:55–68CrossRefGoogle Scholar
  29. Kolar SK, Lodge DM (2002) Ecological predictions and risk assessment for alien fishes in North America. Science 298:1233–1236PubMedCrossRefGoogle Scholar
  30. Lee CE (2002) Evolutionary genetics of invasive species. Trends Ecol Evol 17:386–391CrossRefGoogle Scholar
  31. Lee CE, Remfert JL, Chang Y-M (2007) Response to selection and evolvability of invasive populations. Genetica 129:179–192PubMedCrossRefGoogle Scholar
  32. Lindholm AK, Breden F, Alexander HJ, Chan W-K, Thakurta SG, Brooks R (2005) Invasion success and genetic diversity of introduced populations of guppies Poecilia reticulata in Australia. Mol Ecol 14:3671–3682PubMedCrossRefGoogle Scholar
  33. Loh R, Bitner-Mathé BC (2005) Variability of wing size and shape in three populations of a recent Brazilian invader, Zaprionus indianus (Diptera: Drosophilidae), from different habitats. Genetica 125:271–281PubMedCrossRefGoogle Scholar
  34. Lyytinen A, Lindström L, Mappes J, Julkunen-Tiitto R, Fasulati S, Tiilikkala K (2007) Variability in host plant chemistry: behavioral responses and life-history parameters of the Colorado Potato Beetle (Leptinotarsa decemlineata). Chemoecology 17:51–56CrossRefGoogle Scholar
  35. Mappes J, Kaitala A, Rinne V (1996) Temporal variation in reproductive allocation in a shield bug (Elasmostethus interstinctus). J Zool 240:29–35CrossRefGoogle Scholar
  36. Mousseau TA, Dingle H (1991) Maternal effects in insect life histories. Annu Rev Entomol 36:511–534CrossRefGoogle Scholar
  37. Mousseau TA, Fox CW (1998) The adaptive significance of maternal effects. Trends Ecol Evol 13:403–407CrossRefGoogle Scholar
  38. Noronha C, Cloutier C (1998) Effect of soil conditions and body size on digging by prediapause Colorado potato beetles (Coleoptera: Chrysomelidae). Can J Zool 76:1705–1713CrossRefGoogle Scholar
  39. Nylin S, Wiklund C, Wickman PO, Garcia-Barros E (1993) Absence of trade-offs between sexual size dimorphism and early male emergence in a butterfly. Ecology 74:1414–1427CrossRefGoogle Scholar
  40. Parker IM, Rodriguez J, Loik ME (2003) An evolutionary approach to understanding the biology of invasions: local adaptation and general-purpose genotypes in the weed Verbascum thapsus. Conserv Biol 17:59–72CrossRefGoogle Scholar
  41. Peterson AT (2003) Predicting the geography of species’ invasions via ecological niche modeling. Q Rev Biol 78:419–433PubMedCrossRefGoogle Scholar
  42. Roff DA (1992) The evolution of life-histories: theory and analysis. Chapman and Hall, New YorkGoogle Scholar
  43. Roff DA (1997) Evolutionary quantitative genetics. Chapman and Hall, New YorkGoogle Scholar
  44. Roura-Pascual N, Suarez AV, Gómez C, Pons P, Toyama Y, Wild AL, Peterson AT (2004) Geographical potential of Argentine ants (Linepithema humile Mayr) in the face of global climate change. Proc R Soc Lond Biol Ser B 271:2527–2534CrossRefGoogle Scholar
  45. Sakai AK, Allendorf FW, Holt JS, Lodge DM, Molofsky J, With KA, Baughman S, Cabin RJ, Cohen JE, Ellstrand NC (2001) The population biology of invasive species. Annu Rev Ecol Evol Syst 32:305–332CrossRefGoogle Scholar
  46. Sax DF, Stachowicz JJ, Gaines SD (eds) (2005) Species invasions: insights into ecology, evolution, and biogeography. Sinauer Associates, SunderlandGoogle Scholar
  47. Sgró CM, Hoffmann AA (2004) Genetic correlations, tradeoffs and environmental variation. Heredity 93:241–248PubMedCrossRefGoogle Scholar
  48. Tsutsui ND, Suarez AV, Holway DA, Case TJ (2000) Reduced genetic variation and the success of an invasive species. Proc Natl Acad Sci USA 97:5948–5953PubMedCrossRefGoogle Scholar
  49. Wagner JD, Glover MD, Moseley JB, Moore AJ (1999) Heritability and fitness consequences of cannibalism in Harmonia axyridis. Evol Ecol Res 1:375–388Google Scholar
  50. Wiens JJ, Graham CH (2005) Niche conservatism: integrating evolution, ecology, and conservation biology. Annu Rev Ecol Evol Syst 36:519–539CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Sanna Boman
    • 1
  • Alessandro Grapputo
    • 1
    • 2
  • Leena Lindström
    • 1
  • Anne Lyytinen
    • 1
  • Johanna Mappes
    • 1
  1. 1.Department of Biological and Environmental ScienceUniversity of JyväskyläJyväskyläFinland
  2. 2.Dipartmento di BiologiaUniversity of PadovaPadovaItaly

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