Biological Invasions

, Volume 8, Issue 2, pp 217–230

Two Invasive Plants Alter Soil Microbial Community Composition in Serpentine Grasslands

  • Katharine M. Batten
  • Kate M. Scow
  • Kendi F. Davies
  • Susan P. Harrison


Plant invasions pose a serious threat to native ecosystem structure and function. However, little is known about the potential role that rhizosphere soil microbial communities play in facilitating or resisting the spread of invasive species into native plant communities. The objective of this study was to compare the microbial communities of invasive and native plant rhizospheres in serpentine soils. We compared rhizosphere microbial communities, of two invasive species, Centaurea solstitialis (yellow starthistle) and Aegilops triuncialis (barb goatgrass), with those of five native species that may be competitively affected by these invasive species in the field (Lotus wrangelianus, Hemizonia congesta, Holocarpha virgata, Plantago erecta, and Lasthenia californica). Phospholipid fatty acid analysis (PLFA) was used to compare the rhizosphere microbial communities of invasive and native plants. Correspondence analyses (CA) of PLFA data indicated that despite yearly variation, both starthistle and goatgrass appear to change microbial communities in areas they invade, and that invaded and native microbial communities significantly differ. Additionally, rhizosphere microbial communities in newly invaded areas are more similar to the original native soil communities than are microbial communities in areas that have been invaded for several years. Compared to native plant rhizospheres, starthistle and goatgrass rhizospheres have higher levels of PLFA biomarkers for sulfate reducing bacteria, and goatgrass rhizospheres have higher fatty acid diversity and higher levels of biomarkers for sulfur-oxidizing bacteria, and arbuscular mycorrhizal fungi. Changes in soil microbial community composition induced by plant invasion may affect native plant fitness and/or ecosystem function.


Aegilops triuncialis Centaurea solstitialis plant invasion PLFA serpentine soil microbial community 



Arbuscular mycorrhizal fungi


phospholipid fatty acid


McLaughlin goatgrass site


McLaughlin starthistle site


Bear Valley goatgrass site


correspondence analysis


canonical correspondence analysis


invasive patch center samples


invasive patch edge samples


native samples


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  1. Allen, EB, Allen, MF, Helm, DJ, Trappe, JM, Molina, R, Rincon, E 1995Patterns and regulation of mycorrhizal plant and fungal diversityPlant and Soil1704762CrossRefGoogle Scholar
  2. Al-Zarban, SS, Al-Musallam, AA, Abbas, I, Stackebrandt, E, Kroppenstedt, RM 2002Saccharomonospora halophila sp. nov., a novel halophilic actinomycete isolated from marsh soil in KuwaitInternational Journal of Systematic and Evolutionary Microbiology52555558PubMedGoogle Scholar
  3. Bais, HP, Walker, TS, Stermitz, FR, Hufbauer, RA, Vivanco, JM 2002Enantiomeric-dependent phytotoxic and antimicrobial activity of (±) catechin. A rhizosecreted racemic mixture from spotted knapweedPlant Physiology12811731179PubMedCrossRefGoogle Scholar
  4. Balser, TC 2001The impact of long-term nitrogen addition on microbial community composition in three Hawaiian forest soilsThe Scientific World1500504Google Scholar
  5. Batten, KM 2004Plant invasion and the soil microbial community: interactions and implications for native plant performance and ecosystem function. Department of Land, Air and Water ResourcesUniversity of CaliforniaDavis, California104PhD thesisGoogle Scholar
  6. Belnap, J, Phillips, SL 2001Soil biota in an ungrazed grassland: response to annual grass (Bromus tectorum) invasionEcological Applications1112611275Google Scholar
  7. Bever, JD 2003Soil community feedback and the coexistence of competitors: conceptual frameworks and empirical testsNew Phytologist157465473CrossRefGoogle Scholar
  8. Bonkowski, M, Brandt, F 2002Do soil protozoa enhance plant growth by hormonal effects?Soil Biology and Biochemistry3417091715CrossRefGoogle Scholar
  9. Bonkowski, M, Cheng, W, Griffiths, BS, Alphei, J, Scheu, S 2000Microbial–faunal interactions in the rhizosphere and effects on plant growthEuropean Journal of Soil Biology36135147CrossRefGoogle Scholar
  10. Bossio, DA, Scow, KM 1998Impacts of carbon and flooding on soil microbial communities: phospholipid fatty acid profiles and substrate utilization patternsMicrobial Ecology35265278PubMedCrossRefGoogle Scholar
  11. Callaway, RM, Thelen, GC, Rodriguez, A, Holben, WE 2004Soil biota and exotic plant invasionNature427731733PubMedCrossRefGoogle Scholar
  12. D’Antonio, CM, Vitousek, PM 1992Biological invasions by exotic grasses, the grass/fire cycle and global changeAnnual Review of Ecology and Systematics236387Google Scholar
  13. Deforest, JL, Zak, DR, Pregitzer, KS, Burton, AJ 2004Atmospheric nitrate deposition, microbial community composition, and enzyme activity in northern hardwood forestsSoil Science Society of America Journal68132138Google Scholar
  14. DiTomaso, JM, Gerlach, JD,Jr 2000

    Centaurea solstitialis L

    Bossard, CCRandall, JMHoshovsky, MC eds. Invasive Plants of California’s WildlandsUniversity of California PressBerkeley, California101106
    Google Scholar
  15. Dowling, NJE, Widdel, F, White, DC 1986Phospholipid ester-linked fatty acid biomarkers of acetate-oxidizing sulphate-reducers and other sulphide-forming bacteriaJournal of General Microbiology13218151825Google Scholar
  16. Dowling, NJE, Nichols, PD, White, DC 1988Phospholipid fatty acid and infra-red spectroscopic analysis of a sulphate-reducing consortiumFEMS Microbiology Ecology53325334Google Scholar
  17. Duda, JJ, Freeman, DC, Emlen, JM, Belnap, J, Kitchen, SG, Zak, JC, Sobek, E, Tracy, M, Montante, J 2003Differences in native soil ecology associated with invasion of the exotic annual chenopod, Halogeton glomeratusBiology and Fertility of Soils387277CrossRefGoogle Scholar
  18. Edlund, A, Nichols, PD, Roffey, R, White, DC 1985Extractable and lipopolysaccharide fatty acid and hydroxyl acid profiles from Desulfovibrio speciesJournal of Lipid Research26982988PubMedGoogle Scholar
  19. Ehrenfeld, JG 2003Effects of exotic plant invasions on soil nutrient cycling processesEcosystems6503523CrossRefGoogle Scholar
  20. Eviner, VT, Chapin, FS,III 2002The influence of plant species, fertilization and elevated CO2 on soil aggregate stabilityPlant and Soil246211219CrossRefGoogle Scholar
  21. Glick, BR 1995The enhancement of plant growth by free-living bacteriaCanadian Journal of Microbiology41109117Google Scholar
  22. Grayston, SJ, Wang, S, Campbell, CD, Edwards, AC 1996Selective influence of plant species on microbial diversity in the rhizosphereSoil Biology and Biochemistry30369378Google Scholar
  23. Grayston, SJ, Griffith, GS, Mawdsley, JL, Campbell, CD, Bardgett, RD 2001Accounting for variability in soil microbial communities of temperate upland grassland ecosystemsSoil Biology and Biochemistry33533551CrossRefGoogle Scholar
  24. Holmes, AJ, Roslev, P, McDonald, IR, Iversen, N, Henriksen, K, Murrell, JC 1999Characterization of methanotrophic bacterial populations in soils showing atmospheric methane uptakeApplied and Environmental Microbiology6533123318PubMedGoogle Scholar
  25. Huenneke, L, Hamburg, S, Koide, R, Mooney, H, Vitousek, P 1990Effects of soil resources on plant invasion and community structure in California (USA) serpentine grasslandEcology71478491Google Scholar
  26. Ito, T, Okabe, S, Satoh, H, Watanabe, Y 2002Successional development of sulfate-reducing bacterial populations and their activities in a wastewater biofilm growing under microaerophilic conditionsApplied and Environmental Microbiology6813921402PubMedGoogle Scholar
  27. Kelsey, RG, Locken, LG 1987Phytotoxic properties of cnicin a sesquiterpene lactone from Centaurea maculosa spotted knapweedJournal of Chemical Ecology131934CrossRefGoogle Scholar
  28. Kerger, BD, Nichols, PD, Antworth, CP, Sand, W, Bock, E, Cox, JC, Langworthy, TA, White, DC 1986Signature fatty acids in the polar lipids of acid-producing Thiobacillus spp.: methoxy, cyclopropyl, alpha-hydroxy-cyclopropyl and branched and normal monoenoic fatty acidsFEMS Microbiology Ecology386777Google Scholar
  29. Klironomos, JN 2002Feedback with soil biota contributes to plant rarity and invasiveness in communitiesNature4176770PubMedCrossRefGoogle Scholar
  30. Kourtev, PS, Ehrenfeld, JG, Haggblom, M 2002Exotic plant species alter the microbial community structure and function in the soilEcology8331523166CrossRefGoogle Scholar
  31. Kourtev, PS, Ehrenfeld, JG, Haggblom, M 2003Experimental analysis of the effect of exotic and native plant species on the structure and function of soil microbial communitiesSoil Biology and Biochemistry35895905CrossRefGoogle Scholar
  32. Kruckeberg, AR 1984California serpentines: flora, vegetation, geology, soils and management problemsUniversity of California PressBerkeley, California180Google Scholar
  33. Kuske, CR, Ticknor, LO, Miller, ME, Dunbar, JM, Davis, JA, Barns, SM, Belnap, J 2002Comparison of soil bacterial communities in rhizospheres of three plant species and the interspaces in an arid grasslandApplied and Environmental Microbiology6818541863PubMedCrossRefGoogle Scholar
  34. Lindahl, V, Frostegard, A, Bakken, L, Baath, E 1997Phospholipid fatty acid composition of size fractionated indigenous soil bacteriaSoil Biology and Biochemistry2915651569CrossRefGoogle Scholar
  35. Linos, A, Steinbuchel, A, Sproer, C, Kroppenstedt, RM 1999Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyreInternational Journal of Systematic Bacteriology4917851791PubMedCrossRefGoogle Scholar
  36. Locken, LJ, Kelsey, RG 1987Cnicin concentrations in Centaurea-maculosa spotted knapweedBiochemical Systematics & Ecology15313320Google Scholar
  37. Lyons, KG, Schwartz, MW 2001Rare species loss alters ecosystem function – invasion resistanceEcology Letters4358365CrossRefGoogle Scholar
  38. Macalady, JL, Mack, EE, Nelson, DC, Scow, KM 2000Sediment microbial community structure and mercury methylation in mercury-polluted Clear Lake, CaliforniaApplied and Environmental Microbiology6614791488PubMedCrossRefGoogle Scholar
  39. Marschner, H 1995Mineral nutrition of higher plants2Academic Press, Inc.San Diego, California889Google Scholar
  40. Minz, D, Fishbain, S, Green, SJ, Muyzer, G, Cohen, Y, Rittmann, BE, Stahl, DA 1999Unexpected population distribution in a microbial mat community: sulfate-reducing bacteria localized to the highly oxic chemocline in contrast to a eukaryotic preference for anoxia.Applied and Environmental Microbiology6546594665PubMedGoogle Scholar
  41. Muir, AD, Majak, W 1983Allelopathic potential of diffuse knapweed Centaurea-diffusa extractsCanadian Journal of Plant Science63989996CrossRefGoogle Scholar
  42. Nichols, PD, Smith, GA, Antworth, CP, Hanson, RS, White, DC 1985Phospholipid and lipopolysacharide normal and hydroxyl fatty acids as potential signatures for the methane-oxidizing bacteriaFEMS Microbiology Ecology31327335Google Scholar
  43. Olsson, PA, Baath, E, Jakobsen, I, Soderstrom, B 1995The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soilMycological Research99623629Google Scholar
  44. Peters, A, Johnson, DE, George, MR 1996Barb goatgrass: a threat to California rangelandsRangelands18810Google Scholar
  45. Rejmanek, M 2000Invasive plants: approaches and predictionsAustral Ecology25497506CrossRefGoogle Scholar
  46. Requena, N, Jimenez, I, Toro, M, Barea, JM 1997Interactions between plant-growth-promoting rhizobacteria (PGPR), arbuscular mycorrhizal fungi and Rhizobium spp. in the rhizosphere of Anthyllis cytisoides, a model legume for revegetation in Mediterranean semi-arid ecosystemsNew Phytologist136667677CrossRefGoogle Scholar
  47. Richardson, DM, Allsopp, N, D’Antonio, CM, Milton, SJ, Rejmanek, M 2000Plant invasions – the role of mutualismsBiological Reviews756593PubMedGoogle Scholar
  48. Ridenour, WM, Callaway, RM 2001The relative importance of allelopathy in interference: the effects of an invasive weed on a native bunchgrassOecologia126444450CrossRefGoogle Scholar
  49. Rillig, MC, Wright, SF, Eviner, VT 2002The role of arbuscular mycorrhizal fungi and glomalin in soil aggregation: comparing effects of five plant speciesPlant and Soil238325333CrossRefGoogle Scholar
  50. Schutter, M, Dick, R 2001Shifts in substrate utilization potential and structure of soil microbial communities in response to carbon substratesSoil Biology and Biochemistry3314811491Google Scholar
  51. Stevens, KL 1986Allelopathic polyacetylenes from Centaurea-repens Russian knapweedJournal of Chemical Ecology1212051212CrossRefGoogle Scholar
  52. Stohlgren, TJ, Otsuki, Y, Villa, CA, Lee, M, Belnap, J 2001Patterns of plant invasions: a case example in native species hotspots and rare habitatsBiological Invasions33750CrossRefGoogle Scholar
  53. ter Braak, CJF, Šmilauer, P 1998CANOCO reference manual and user’s guide to CANOCO for Windows: software for canonical community ordination (version 4)Microcomputer PowerIthaca, New York352Google Scholar
  54. Heijden, MGA, Klironomos, JN, Ursic, M, Moutoglis, P, Streitwolf-Engel, R, Boller, T, Wiemken, A, Sanders, IR 1998Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivityNature3966972Google Scholar
  55. Putten, WH, Dijk, C, Peters, BAM 1993Plant-specific soil-borne diseases contribute to succession in foredune vegetationNature3625356CrossRefGoogle Scholar
  56. Vaughn, CE, Jones, MB, Center, DM 1987Sulfur tests on northern California subclover-annual grass pasture surface soilsSoil Science143184191Google Scholar
  57. Vitousek, PM 1990Biological invasions and ecosystem processes: towards an integration of population biology and ecosystem studiesOikos57713Google Scholar
  58. Vitousek, PM, Walker, LR 1989Biological invasion of Myrica faya in Hawai’i: plant demography, nitrogen fixation, ecosystem effectsEcological Monographs59247265Google Scholar
  59. West, HM 1996Influence of arbuscular mycorrhizal infection on competition between Holcus lanatus and Dactylis glomerataJournal of Ecology84429438Google Scholar
  60. Westover, KM, Kennedy, AC, Kelley, SE 1997Patterns of rhizosphere microbial community structure associated with co-occurring plant speciesJournal of Ecology85863873Google Scholar
  61. White, DC, Bobbie, RJ, Nickels, JS, Fazio, SD, Davis, WM 1980Nonselective biochemical methods for the determination of fungal mass and community structure in estuarine detrital microfloraBotanica Marina23239250Google Scholar
  62. White, DC, Stair, JO, Ringelberg, DB 1996Quantitative comparisons of in situ microbial biodiversity by signature biomarker analysisJournal of Industrial Microbiology17185196CrossRefGoogle Scholar
  63. White, DC, Pinkart, HC, Ringelberg, DB 1997

    Biomass measurements: biochemical approaches

    Hurst, CJKundsen, GRMcInerney, MJStetzenbach, LDWalter, MV eds. Manual of Environmental MicrobiologyASM PressWashington, DC91101
    Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Katharine M. Batten
    • 1
  • Kate M. Scow
    • 2
  • Kendi F. Davies
    • 3
  • Susan P. Harrison
    • 3
  1. 1.American Institute of Biological Sciencs NEON Project OfficeWashingtonUSA
  2. 2.Department of Land, Air, and Water ResourcesUniversity of CaliforniaDavisUSA
  3. 3.Department of Environmental Science and PolicyUniversity of CaliforniaDavisUSA

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