Biotransformation of benzaldehyde into l-phenylacetylcarbinol using magnetic nanoparticles-coated yeast cells

  • Mohammad Mahdi Seifi
  • Elham Iranmanesh
  • Mohammad Ali AsadollahiEmail author
  • Ayyoob Arpanaei
Original Research Paper



The yeast cells were coated with Fe3O4 magnetic nanoparticles and employed as biocatalyst for the microbial biotransformation of benzaldehyde into l-phenylacetylcarbinol (l-PAC).


Saccharomyces cerevisiae CEN.PK113-7D yeast cells were coated with magnetic nanoparticles to facilitate the cells separation process. Transmission electron microscopy, powder XRD diffraction, and vibrating sample magnetometer were used to characterize magnetic nanoparticles and magnetic nanoparticle-coated yeast cells. Then the reusability of magnetically recoverable cells in production of l-PAC was investigated. Results show that coating yeast cells with magnetic nanoparticles does not affect their size and structure. Coated cells were also used in seven consecutive batch cycles and no significant reduction for l-PAC titer was observed in any of the cycles.


Coating yeast cells with magnetic nanoparticles enabled rapid separation and reuse of cells in several successive batch cycle without affecting their ability to produce l-PAC.


Biotransformation Saccharomyces cerevisiae l-Phenylacetylcarbinol Magnetic nanoparticles 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Ansari F, Grigoriev P, Libor S, Tothill IE, Ramsden JJ (2009) DBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticles. Biotechnol Bioeng 102:1505–1512CrossRefGoogle Scholar
  2. Bardania H, Raheb J, Mohammad-Beigi H, Rasekh B, Arpanaei A (2013) Desulfurization activity and reusability of magnetite nanoparticle-coated Rhodococcus erythropolis FMF and R. erythropolis IGTS8 bacterial cells. Biotechnol Appl Biochem 60:323–329CrossRefGoogle Scholar
  3. Bardania H, Raheb J, Arapanei A (2019) Investigation of desulfurization activity, reusability, and viability of magnetite coated bacterial cells. Iran J Biotechnol 17:e2108PubMedPubMedCentralGoogle Scholar
  4. Chen CC, Lan CC, Pan CL, Huang MY, Chew CH, Hung CC, Chen PH, Lin HTV (2019) Repeated-batch lactic acid fermentation using a novel bacterialimmobilization technique based on a microtube array membrane. Process Biochem 87:25–32CrossRefGoogle Scholar
  5. Donia A, Atia A, Abouzayed F (2012) Preparation and characterization of nano-magnetic cellulose with fast kinetic properties towards the adsorption of some metal ions. Chem Eng J 191:22–30CrossRefGoogle Scholar
  6. Doostmohammadi M, Esfandiari M, Asadollahi MA, Kamali M, Nahvi I (2015) Biotransformation of benzaldehyde to L-phenylacetyl carbinol using immobilized cells of Saccharomyces cerevisiae. Minerva Biotecnol 27:43–49Google Scholar
  7. Doostmohammadi M, Asadollahi MA, Nahvi I, Biria D, Ghezelbash GR, Kheyrandish M (2016) L-phenylacetylcarbinol production by yeast petite mutants. Ann Microbiol 66:1049–1055CrossRefGoogle Scholar
  8. Ebrahiminezhad A, Varma V, Yang S, Berenjian A (2016) Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation. Appl Microbiol Biotechnol 100:173–180CrossRefGoogle Scholar
  9. Iranmanesh E, Asadollahi MA, Biria D (2020) Improving L-phenylacetylcarbinol production in Saccharomyces cerevisiae by in silico aided metabolic engineering. J Biotechnol 308:27–34CrossRefGoogle Scholar
  10. Kalantari M, Kazemeini M, Arapanaei A (2013) Facile fabrication and characterization of amino-functionalized Fe3O4 cluster@SiO2 core/shell nanocomposite spheres. Mater Res Bull 48:2023–2028CrossRefGoogle Scholar
  11. Khan MA, Ikram U-H, Javed MM, Qadeer MA, Akhtar N, Bokhari SAI (2012) Studies on the production of L-phenylacetylcarbinol by Candida utilis in shake flask. Pak J Bot 44:361–364Google Scholar
  12. Li GY, Huang KL, Jiang YR, Yang DL, Ding P (2008) Preparation and characterization of Saccharomyces cerevisiae alcohol dehydrogenase immobilized on magnetic nanoparticles. Int J Biol Macromol 42:405–412CrossRefGoogle Scholar
  13. Liao M-H, Chen D-H (2001) Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability. Biotechnol Lett 23:1723–1727CrossRefGoogle Scholar
  14. Mandwal A, Tripathi C, Trivedi P, Joshi A, Agarwal S, Bihari V (2004) Production of L-phenylacetyl carbinol by immobilized cells of Saccharomyces cerevisiae. Biotechnol Lett 26:217–221CrossRefGoogle Scholar
  15. Mukherjee AK, Kumar TS, Rai SK, Roy JK (2010) Statistical optimization of Bacillus alcalophilus α-amylase immobilization on iron-oxide magnetic nanoparticles. Biotechnol Bioprocess Eng 15:984–992CrossRefGoogle Scholar
  16. Park JK, Lee KD (2001) Production of L-phenylacetylcarbinol (l-PAC) by encapsulated Saccharomyces cerevisiae cells. Korean J Chem Eng 18:363–370CrossRefGoogle Scholar
  17. Petcharoen K, Sirivat A (2012) Synthesis and characterization of magnetite nanoparticles via the chemical co-precipitation method. Mater Sci Eng B 177:421–427CrossRefGoogle Scholar
  18. Ranmadugala D, Ebrahiminezhad A, Manley-Harris M, Ghasemi Y, Berenjian A (2018) Magnetic immobilization of bacteria using iron oxide nanoparticles. Biotechnol Lett 40:237–248CrossRefGoogle Scholar
  19. Rogers P, Shin H, Wang B (1997) Biotransformation for L-ephedrine production. In: Scheper T (ed) Biotreatment downstream processing and modelling. Springer, Heidelberg, pp 33–59CrossRefGoogle Scholar
  20. Rosche B, Leksawasdi N, Sandford V, Breuer M, Hauer B, Rogers P (2002) Enzymatic (R)-phenylacetylcarbinol production in benzaldehyde emulsions. Appl Microbiol Biotechnol 60:94–100CrossRefGoogle Scholar
  21. Shan G, Xing J, Zhang H, Liu H (2005) Biodesulfurization of dibenzothiophene by microbial cells coated with magnetite nanoparticles. Appl Environ Microbiol 71:4497–4502CrossRefGoogle Scholar
  22. Shaw S-Y, Chen Y-J, Ou J-J, Ho L (2006) Preparation and characterization of Pseudomonas putida esterase immobilized on magnetic nanoparticles. Enzyme Microb Technol 39:1089–1095CrossRefGoogle Scholar
  23. Vaghari H, Jafarizadeh-Malmiri H, Mohammadlou M, Berenjian A, Anarjan N, Jafari N, Nasiri S (2016) Application of magnetic nanoparticles in smart enzyme immobilization. Biotechnol Lett 38:223–233CrossRefGoogle Scholar
  24. Wu W, He Q, Jiang C (2008) Magnetic iron oxide nanoparticles: synthesis and surface functionalization strategies. Nanoscale Res Lett 3:397–415CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2020

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of Biological Science and TechnologyUniversity of IsfahanIsfahanIran
  2. 2.Department of Industrial and Environmental BiotechnologyNational Institute of Genetic Engineering and BiotechnologyTehranIran

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