Advertisement

Improving dark fermentative hydrogen production through zero-valent iron/copper (Fe/Cu) micro-electrolysis

Abstract

Objectives

To investigate the effect of zero-valent iron and copper (Fe/Cu) micro-electrolysis on dark fermentative hydrogen production from glucose by a mixed bacterial consortium and the possible mechanisms of increasing hydrogen yield.

Results

Compared to zero-valent iron and activated carbon (Fe/C) micro-electrolysis, Fe/Cu micro-electrolysis could increase hydrogen yield by 32.2%, hydrogen production potential by 27.1%, and the maximum hydrogen production rate by 62.0%. Meanwhile, the number of ferrous ions released into the liquid phase with Fe/Cu micro-electrolysis was about 27.0% greater than that released by Fe/C micro-electrolysis, because the dispersion of copper on the surface of iron could markedly improve electrochemical corrosion activity. Metabolic analysis revealed that Fe/C micro-electrolysis promoted acetate formation, which may have been responsible for the observed improvement in fermentative hydrogen production. Further investigation indicated that Fe/Cu micro-electrolysis increased the activity of hydrogenases and stimulated the expression of the [FeFe]-hydrogenase gene.

Conclusion

Fe/Cu micro-electrolysis is better than Fe/C micro-electrolysis or Fe corrosion alone for dark fermentative hydrogen production.

This is a preview of subscription content, log in to check access.

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Fig. 1
Fig. 2
Fig. 3

References

  1. Andersch W, Bahl H, Gottschalk G (1983) Level of enzymes involved in acetate, butyrate, acetone and butanol formation by Clostridium acetobutylicum. Eur J Appl Microbiol Biot 18:327–332. https://doi.org/10.1007/bf00504740

  2. Chen K-F, Li S, Zhang W-x (2011) Renewable hydrogen generation by bimetallic zero valent iron nanoparticles. Chem Eng J 170:562–567. https://doi.org/10.1016/j.cej.2010.12.019

  3. Guan X, Sun Y, Qin H, Li J, Lo IMC, He D, Dong H (2015) The limitations of applying zero-valent iron technology in contaminants sequestration and the corresponding countermeasures: the development in zero-valent iron technology in the last two decades (1994–2014). Water Res 75:224–248. https://doi.org/10.1016/j.watres.2015.02.034

  4. Huang Y, Zong W, Yan X, Wang R, Hemme CL, Zhou J, Zhou Z (2010) Succession of the bacterial community and dynamics of hydrogen producers in a hydrogen-producing bioreactor. Appl Environ Microb 76:3387. https://doi.org/10.1128/AEM.02444-09

  5. Huang Z, Yu X, Miao H, Ren H, Zhao M, Ruan W (2012) Enzymatic dynamics of microbial acid tolerance response (ATR) during the enhanced biohydrogen production process via anaerobic digestion. Int J Hydrogen Energy 37:10655–10662. https://doi.org/10.1016/j.ijhydene.2012.04.116

  6. Laurinavichene T, Laurinavichius K, Shastik E, Tsygankov A (2018) Long-term H2 photoproduction from starch by co-culture of Clostridium butyricum and Rhodobacter sphaeroides in a repeated batch process. Biotechnol Lett 40:309–314. https://doi.org/10.1007/s10529-017-2486-z

  7. Lenhard G (1968) A standardized procedure for the determination of dehydrogenase activity in samples from anaerobic treatment systems. Water Res 2:161–167. https://doi.org/10.1016/0043-1354(68)90033-X

  8. Ma LM, Ding ZG, Gao TY, Zhou RF, Xu WY, Liu J (2004) Discoloration of methylene blue and wastewater from a plant by a Fe/Cu bimetallic system. Chemosphere 55:1207–1212. https://doi.org/10.1016/j.chemosphere.2003.12.021

  9. Mu Y, Wang G, Yu H-Q (2006) Kinetic modeling of batch hydrogen production process by mixed anaerobic cultures. Bioresource Technol 97:1302–1307. https://doi.org/10.1016/j.biortech.2005.05.014

  10. Nicolaou SA, Gaida SM, Papoutsakis ET (2010) A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: from biofuels and chemicals, to biocatalysis and bioremediation. Metab Eng 12:307–331. https://doi.org/10.1016/j.ymben.2010.03.004

  11. Ntaikou I, Antonopoulou G, Lyberatos G (2010) Biohydrogen production from biomass and wastes via dark fermentation: a review. Waste Biomass Valoriz 1:21–39. https://doi.org/10.1007/s12649-009-9001-2

  12. Tang P, Deng C, Tang X, Si S, Xiao K (2012) Degradation of p-nitrophenol by interior microelectrolysis of zero-valent iron/copper-coated magnetic carbon galvanic couples in the intermittent magnetic field. Chem Eng J 210:203–211. https://doi.org/10.1016/j.cej.2012.08.089

  13. Wittkamp F, Senger M, Stripp ST, Apfel UP (2018) [FeFe]-Hydrogenases: recent developments and future perspectives. Chem Commun 54:5934–5942. https://doi.org/10.1039/C8CC01275J

  14. Wong YM, Wu TY, Juan JC (2014) A review of sustainable hydrogen production using seed sludge via dark fermentation. Renew Sustain Energy Rev 34:471–482. https://doi.org/10.1016/j.rser.2014.03.008

  15. Yang H, Shen J (2006) Effect of ferrous iron concentration on anaerobic bio-hydrogen production from soluble starch. Int J Hydrogen Energy 31:2137–2146. https://doi.org/10.1016/j.ijhydene.2006.02.009

  16. Zhang L, Zhang L, Li D (2015) Enhanced dark fermentative hydrogen production by zero-valent iron activated carbon micro-electrolysis. Int J Hydrogen Energy 40:12201–12208. https://doi.org/10.1016/j.ijhydene.2015.07.106

  17. Zhao X, Xing D, Liu B, Lu L, Zhao J, Ren N (2012) The effects of metal ions and l-cysteine on hydA gene expression and hydrogen production by Clostridium beijerinckii RZF-1108. Int J Hydrogen Energy 37:13711–13717. https://doi.org/10.1016/j.ijhydene.2012.02.144

  18. Zhu H, Seto P, Parker WJ (2014) Enhanced dark fermentative hydrogen production under the effect of zero-valent iron shavings. Int J Hydrogen Energy 39:19331–19336. https://doi.org/10.1016/j.ijhydene.2014.06.055

Download references

Author information

Correspondence to Lei Zhang.

Ethics declarations

Conflict of interest

There is no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, L., Xu, D., Kong, D. et al. Improving dark fermentative hydrogen production through zero-valent iron/copper (Fe/Cu) micro-electrolysis. Biotechnol Lett 42, 445–451 (2020). https://doi.org/10.1007/s10529-020-02793-5

Download citation

Keywords

  • Dark fermentation
  • Fe/Cu micro-electrolysis
  • Hydrogen production
  • Zero-valent iron