Discovery and characterization of a stable lipase with preference toward long-chain fatty acids
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To identify novel lipases with stability and long-chain fatty acids preference by phylogenetic evolution analysis methods from database.
Thermo-stable Candida antarctica Lipase-A (CALA) was set as a template for gene mining by PSI-BLAST. Based on phylogenetic analysis, three candidate lipases exhibiting 97%, 55%, and 35% identities with CALA, respectively, were selected for overexpression and characterization. Lipase, PhLip from Pseudozyma hubeiensis SY62 showed highest activity towards long-chain fatty acids, and showed maximum activity at pH 9.0 and 60 °C, and stability between 40 and 50 °C for 4 h and at pH 7–10 for 12 h. Enzymatic hydrolysis of Mucor circinelloides WJ11 oils by PhLip was about twofold higher than that by CALA, with respect to hydrolysis of long-chain fatty acids. Besides, fatty acids with 18 carbons, including oleic acid, linoleic acid, and linolenic acid, were preferred as substrates.
The current investigation discovered a stable lipase PhLip with long-chain fatty acids preference. PhLip may be a potential candidate for producing polyunsaturated fatty acids from natural oils.
KeywordsCALA Enrichment Lipase Mucor circinelloides Phylogeny analysis Unsaturated fatty acid
We would like to thank Editage (www.editage.com) for English language editing.
This work was supported by the National Natural Science Foundation of China (Grant Nos. 31670064, 31972851 and 21402109), Taishan Industry Leading Talent Project (Grant No. LJNY201606).
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Conflict of interest
All authors declare that they have no conflict of interest.