Biotechnology Letters

, Volume 41, Issue 12, pp 1433–1437 | Cite as

Violacein@Biogenic Ag system: synergistic antibacterial activity against Staphylococcus aureus

  • Gerson NakazatoEmail author
  • Marcelly Chue Gonçalves
  • Meiriele da Silva das Neves
  • Renata Katsuko Takayama Kobayashi
  • Marcelo Brocchi
  • Nelson Durán
Original Research Paper



To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis.


Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were evaluated in antimicrobial tests.


E. coli isolates were not inhibited by violacein at concentrations up to 400 μM and they showed sensitivity for AgNPs between 62.5 and 250 μM. Staphylococcus aureus showed sensitivity to violacein with MIC of 200 μM, and the MIC with AgNPs between 250 μM and 125 μM. It was also tested the association between the two compounds through a concentration gradient and was observed the reduction of the MIC in the combination for both strains.


The bactericidal effect of violacein against S. aureus was better when combined with AgNPs (synergistic).


Escherichia coli Staphylococcus aureus Antimicrobials Silver nanoparticles Violacein 



This study was supported by the National Council for Scientific and Technological Development (CNPq), by the Coordination for the Improvement of higher education personnel (CAPES) of the Brazilian federal government, by the Londrina State University (UEL) and by Campinas State University (UNICAMP)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Biology Sciences CenterLondrina State University (UEL)LondrinaBrazil
  2. 2.Laboratory of Tropical Diseases, Department of Genetic, Evolution and Bioagents, Biology InstituteUniversity of Campinas (UNICAMP)CampinasBrazil
  3. 3.Laboratory of Urogenital Carcinogenesis and Immunotherapy, Biological Institute, Department of Structural and Functional BiologyUniversity of CampinasCampinasBrazil
  4. 4.NanoBioss, Institute of ChemistryUniversity of CampinasCampinasBrazil
  5. 5.Nanomed CenterFederal University of ABCSanto AndréBrazil

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