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Biotechnology Letters

, Volume 41, Issue 12, pp 1415–1421 | Cite as

A food-grade engineered Lactococcus lactis strain delivering Helicobacter pylori Lpp20 alleviates bacterial infection in H. pylori-challenged mice

  • Nan Sun
  • Rongguang ZhangEmail author
  • Guangcai Duan
  • Xiaoyan Peng
  • Chen Wang
  • Shuaiyin Chen
  • Qingtang Fan
Original Research Paper
  • 89 Downloads

Abstract

Objective

To construct a food-grade bacterium producing and delivering H. pylori Lpp20 antigen and evaluate its immune efficacy against H. pylori challenges with aim to develop anti-H. pylori oral vaccines and functional foods.

Results

Lpp20 was expressed as a 22 kDa protein in Lactococcus lactis, constituting 11.2% of the cell lysate proteins, and recognized by mouse antisera. Mice orally gavaged with the engineered bacterium had elevated serum IgG levels and lowered urease activity of stomach following H. pylori challenges.

Conclusions

This study firstly reports a food-grade L. lactis strain delivering Lpp20 to mucosal immunization sites, demonstrating a novel efficient production and safe utilization mode of Lpp20, offering a promising vaccine candidate and health food sources.

Keywords

Food-grade bacterium Functional food Genetically engineering Helicobacter pylori Lactococcus lactis Oral vaccination 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81773495) and National Science and Technology Major Program of China (2018ZX10301407).

Supporting information

Supplementary Table 1 Bacteria and plasmid vectors used herein.

Author contributions

NS, RZ and GD designed the project and wrote the manuscript. NS, XP and CW engineered the L. lactis strain and carried out the westernblot analysis and animal experimentation. SC and QF performed data analysis and conducted animal experiments.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

Supplementary material

10529_2019_2740_MOESM1_ESM.pdf (61 kb)
Supplementary material 1 (PDF 61 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Nan Sun
    • 1
  • Rongguang Zhang
    • 1
    Email author
  • Guangcai Duan
    • 1
  • Xiaoyan Peng
    • 1
  • Chen Wang
    • 1
  • Shuaiyin Chen
    • 1
  • Qingtang Fan
    • 1
  1. 1.Department of Epidemiology and Statistics, College of Public HealthZhengzhou UniversityZhengzhouChina

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