A food-grade engineered Lactococcus lactis strain delivering Helicobacter pylori Lpp20 alleviates bacterial infection in H. pylori-challenged mice
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To construct a food-grade bacterium producing and delivering H. pylori Lpp20 antigen and evaluate its immune efficacy against H. pylori challenges with aim to develop anti-H. pylori oral vaccines and functional foods.
Lpp20 was expressed as a 22 kDa protein in Lactococcus lactis, constituting 11.2% of the cell lysate proteins, and recognized by mouse antisera. Mice orally gavaged with the engineered bacterium had elevated serum IgG levels and lowered urease activity of stomach following H. pylori challenges.
This study firstly reports a food-grade L. lactis strain delivering Lpp20 to mucosal immunization sites, demonstrating a novel efficient production and safe utilization mode of Lpp20, offering a promising vaccine candidate and health food sources.
KeywordsFood-grade bacterium Functional food Genetically engineering Helicobacter pylori Lactococcus lactis Oral vaccination
This work was supported by the National Natural Science Foundation of China (81773495) and National Science and Technology Major Program of China (2018ZX10301407).
Supplementary Table 1 Bacteria and plasmid vectors used herein.
NS, RZ and GD designed the project and wrote the manuscript. NS, XP and CW engineered the L. lactis strain and carried out the westernblot analysis and animal experimentation. SC and QF performed data analysis and conducted animal experiments.
Compliance with ethical standards
Conflict of interest
The authors declare no competing interests.