A kinetic model to optimize and direct the dose ratio of Dsz enzymes in the 4S desulfurization pathway in vitro and in vivo
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To enhance the biodesulfurization rate using a kinetic model that directs the ratio of Dsz enzymes.
This study established a kinetic model that predicted the optimal ratio of Dsz enzymes in the 4S biodesulfurization system to be A:B:C = 1:2:4 and 1:4:2. When BCAD+1A+4B+2C, the conversion rate of dibenzothiophene (DBT) to 2-hydroxybiphenyl (HBP) was close to 100% in vitro. When the gene dose of dszC was increased, the HBP yield of the recombinant strain BL21(DE3)/BCAD + C reached approximately 0.012 mM in vivo, which was approximately 6-fold higher than that of the BCAD strain.
According to the results predicted by the enzyme kinetic model, maintaining higher concentrations of DszC and DszB in the desulfurization system can effectively improve the desulfurization efficiency.
KeywordsBiodesulfurization Kinetic model Dsz enzyme ratio Gene dosagen
2-(2-Hydroxybiphenyl) 2 sulfinic acid salt
High-performance liquid chromatography
Supplementary Figure 1. The rate of conversion of DBT to HBP by BL21(DE3)/BADC+C.
This research was financially supported by the National Natural Science Foundation of China (Grant No. 31470159), the National Science Foundation for Young Scientists of China (Grant No. 31400062).
Compliance with ethical standards
Conflict of interest
The authors declare no competing financial interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
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