Antibacterial mode of fibrauretine and synergistic effect with kanamycin against multi-drug resistant Escherichia coli
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The present study evaluated the antibacterial activity and mode of action of fibrauretine on Escherichia coli (E. coli) and Staphylococcus aureus, and synergistic effect with kanamycin against multi-drug resistant E. coli.
The fibrauretine exhibited inhibitory effect on the growth of the tested bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration of 2.5–5 and 5–10 mg/ml, respectively. Morphological changes of cell microstructure were observed after adding fibrauretine at MIC. The mode of action was further confirmed by measuring release of 260-nm absorbing materials and extracellular potassium ions. Checkerboard dilution test suggested that fibrauretine exhibited synergistic activity when combined with kanamycin (FICI ranging from 0.5625 to 0.625).
Our results indicated that fibrauretine exerted synergistic effect with kanamycin and its antibacterial mode of action mainly attributed to disruption of cell membrane integrity.
KeywordsFibrauretine Antibacterial activity Membrane integrity Synergistic effect
This work was supported by the National Key Research and Development Plan (Grant No. 2016YFD0501301); the National Natural Science Foundation of China (Grant No. 31702293); the Science and Technology Development Plan of Jilin Province (Grant No. 20170520074JH) and the Natural Science Foundation of Jilin Province (Grant No. 20180101003JC).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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